Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F74 I1 R1	23	45.7	1836232	90.6%	1663626	141.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	760,485	G→A	G594S (GGC→AGC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	760,485	0	G	A	89.2%	83.9 / NA	38	G594S (GGC→AGC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (16/17); total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760348‑760624

aaGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGt                                                                                                                                        >  1:438952/1‑143 (MQ=255)

   ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGGTGGCTGCCGGTGTGAAACTGTTCGACTGGAGCGGTGcc                                                                                                                                     >  1:487256/1‑142 (MQ=255)

   ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCg                                                                                                                                     <  1:715400/143‑1 (MQ=255)

    tGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCGCGGGGCCGGAAGCAGTTGAAATGCAGTCTCGCGTGGCCAGGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCgg                                                                                                                                    <  2:416045/143‑1 (MQ=255)

        gtggcgcgcGTGCAGGAGCTGGGGAAACGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGTGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaaa                                                                                                                                <  2:805180/138‑1 (MQ=255)

        aTGAAGCGCCGGCAGGGGCGGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGGGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaaa                                                                                                                                <  2:376261/143‑1 (MQ=255)

               gcCGGCAGGAGCTGGCGAAACGCATCGGCGCGGTGCTGGGAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCt                                                                                                                         <  2:65692/143‑1 (MQ=255)

                    cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTGCGGAAACCCTCGCTTACGc                                                                                                                    >  1:814234/1‑143 (MQ=255)

                            ggCGGAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTgg                                                                                                            <  2:663200/143‑1 (MQ=255)

                                aaaCGCATCTGCACGGTCCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga                                                                                                        <  2:804538/143‑1 (MQ=255)

                                     cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg                                                                                                   <  1:79789/143‑1 (MQ=255)

                                     cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCCCGCTTACGCCACGCTGGTTGATGAAg                                                                                                   >  1:88880/1‑143 (MQ=255)

                                           cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGGGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGGTGAAGGCAttg                                                                                             >  1:374252/1‑142 (MQ=255)

                                             cGGTGCCGGAAGCAGTCGAAATGCAGGCTCGCGTTGCCAAGATTTCTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCg                                                                                           <  2:796416/143‑1 (MQ=255)

                                              ggTGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCACGCGATTGCTGCCGGGGAGAAACTGTGCGACTGGAGCGGTGCGGAACACCTCGCTTACGCCACCCTGGTTGCTGAAGGCATTCCgg                                                                                          >  1:408451/1‑143 (MQ=255)

                                              ggTGCCGGAAGCAGGTGAAATGCAGGCGCGCGTTGACTGTAGTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCgg                                                                                          <  2:643807/143‑1 (MQ=255)

                                                  ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGGTGAGAGGATACCAGTTCg                                                                                      >  2:553581/1‑143 (MQ=255)

                                                   gggagtcAGTTGAAATGGCGTCTGGCGCTGCGAAGAATTACGGCGCGCGCCGGGCGATGGCTGCCGGTGAGAACCTGTTCGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                     <  2:324496/137‑1 (MQ=255)

                                                    ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCAAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCACGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCgac                                                                                    >  2:347258/1‑141 (MQ=255)

                                                           gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTGGAGCGGTGCGGAAAACCTCGCTTACGCGACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg                                                                             >  1:638188/1‑143 (MQ=255)

                                                                  tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGAAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc                                                                      >  1:438576/1‑143 (MQ=255)

                                                                            cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGGGGTGGGGACAACCTCGCTCAAGCCACGCTGGTTGATGAAGGCATTCTGGTTCGGCTGTAGGGTGACGACGCGGGTcgcg                                                            >  2:364466/1‑143 (MQ=255)

                                                                                gCCAAGATTTATGGCGATCGCCAGGCGATGGATGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGGTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc                                                        >  1:747244/1‑143 (MQ=255)

                                                                                   aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTTCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTGCctt                                                     >  1:771220/1‑143 (MQ=255)

                                                                                        gtATGGCGCCCGCCGGGCGATGGCTGCCAGGACGAAACGGTCTGCCGGGAGCGGTGCGGAAAACCTCCCTCCGGTTATGAACGTTCTTGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGATTCCGGTCGCGGTACCTTCTTcc                                                <  2:649630/142‑1 (MQ=255)

                                                                                               gATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCCACTGCCGCGGTGCGGCAACCCTCGCCTACGCCACGCTGGCTGGACAAGGCACGCGGAGCCACCCGTCGGGTGAACACCCCGGGCGCGGTCCCCCCGTCCACCGCCa                                         >  2:173510/1‑143 (MQ=255)

                                                                                                    cgaGGCGGTGGCAGCCGGTGTGGTGCGGTGCGTCTGGAGCGGGGCGGACTACGGTGCTTGCGCGAGGCTGGTTGATTATGGCATTCCGGTTCGCCTGGCGGGTGAAGACTCCGGTCGCCGTACTTTCTTCCACCGCCACGCgg                                    <  2:226513/141‑1 (MQ=255)

                                                                                                    ccgggCGCTGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCgg                                    <  2:286091/140‑1 (MQ=255)

                                                                                                    ccAGGCGGTGGCTGCCGGGGGGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCgg                                    <  2:685273/143‑1 (MQ=255)

                                                                                                           aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc                                                        <  1:388354/116‑1 (MQ=255)

                                                                                                           aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc                                                        >  2:388354/1‑116 (MQ=255)

                                                                                                                tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCCCCGCCACGCGCTGATCCCCCAcc                        >  1:748981/1‑143 (MQ=255)

                                                                                                                 gCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTGGCGGTCCCTTGTTCCACCGCCACGGGGTGCTCCGCACCCa                       >  2:626218/1‑143 (MQ=255)

                                                                                                                        agaACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCTGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc                <  2:420962/143‑1 (MQ=255)

                                                                                                                         gaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACCACCAGTCTCACg               >  1:218092/1‑143 (MQ=255)

                                                                                                                                 tCGGCTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACt       <  2:88880/143‑1 (MQ=255)

                                                                                                                                      tGGGGCGGTGCGGAAAACCTCGCTTACGCCTTCCGGGTTGATGAAGGCATTCCCGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac  <  2:438576/143‑1 (MQ=255)

                                                                                                                                      tGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac  >  1:492390/1‑143 (MQ=255)

AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC  >  NC_000913/760348‑760624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A