Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F124 I1 R1
|
28 |
40.1 |
1601150 |
89.4% |
1431428 |
142.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 97.0%
| 82.9
/ NA
| 34 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (13/19); minor base C (1/0); total (14/20) |
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621
|
cAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCg > 1:544638/1‑143 (MQ=255)
tggaGCGCCGGCAGTAGCGGGCGAAACGCATCAGCACGGTGCCGGCAGCAGTTGAAAGGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAc < 2:307432/140‑1 (MQ=255)
tGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAGTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTACGGAAAAc > 2:455360/1‑143 (MQ=255)
aGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCGCGCGTTGCCAAGCTCTATGGACATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 2:387978/143‑1 (MQ=255)
ggAGCTGGCGCAACGCATCAGCACGGTGGGGGAAGCAGTTGACATGCAGTCTCGCGTTGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCa < 2:669241/143‑1 (MQ=255)
ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCACCCCTCGCTTACCCCa > 1:344292/1‑143 (MQ=255)
ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCa > 1:631906/1‑143 (MQ=255)
cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGGTCGACTGGAGCGGTGCGGAAAACCTCGCTTAcaccacacc > 2:94116/1‑140 (MQ=255)
gCGAAACGCATCCGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑Gt < 1:75935/143‑1 (MQ=255)
cGAAGCGTATCAGCACGGTGCCGGAAGCAGTTGGAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGAGGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑Gtt < 2:97257/143‑1 (MQ=255)
cgggggcGGAAGCAGTTGAGATGCAGGCGCGCGTTGCCAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCg < 1:24506/137‑1 (MQ=255)
gcgggAAGCGGTTGAAATGCGGTCGAGCGTTGCTAAGGTTTATGGCGATCGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTc < 2:36345/140‑1 (MQ=255)
gTTGAAATTCAGTCTCGCGTGTCCACAATTTATGGCCATCGCCAGGCGATCGCTGCGGCTGAGAAACTCTTCGACTGTAGCGTTGCTGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTTTCggg < 2:549997/143‑1 (MQ=255)
gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATCAAGGCATTCCGGTTGGCCTGTCggg > 2:783423/1‑143 (MQ=255)
tGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTg < 1:616690/143‑1 (MQ=255)
gcgtGCGGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:160738/140‑1 (MQ=255)
tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGTGAAACTCTTCGACTGGAGCGGTGCGGAACACCTAGCTTGTGGTA‑GCGGCGATGATGCCGGCATTCCGCGTCGCCTGTCGGGTGGAGAc > 2:315149/1‑143 (MQ=255)
gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCg < 1:562650/143‑1 (MQ=255)
gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCg < 2:448177/143‑1 (MQ=255)
tcGCGTTGCCAAGATTTATGGCGATCGCCACGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCCGAACACCTCCATTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCAGTCGGGTGAAGACTCCggac > 2:16341/1‑141 (MQ=255)
cgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg < 1:641379/143‑1 (MQ=255)
cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACAACCTCGCTTACGCCACGCTC‑GTTGATGAAGGCACTCCGGTTCGCCTGCCGGGTGAAGACTCAGGTCGCGGTACct > 2:604225/1‑143 (MQ=255)
aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGCTACctt > 2:144110/1‑143 (MQ=255)
aTGGCGATCGCCAGTCGATGGCTGCCGCTGAGTCAGTGTTCGACTGGAGCGGTGCGGAAAACCGCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:752824/143‑1 (MQ=255)
gCGATGGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGc < 2:506968/143‑1 (MQ=255)
ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 2:227090/143‑1 (MQ=255)
aTGGGTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCca < 2:648030/143‑1 (MQ=255)
tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGCTCGCCTGTCGGGTGAAGAGTCCGGTGGCGGTCCCTTCTTCCACCCCCACGCGGTGAGCCACAGcc > 2:180573/1‑143 (MQ=255)
gCCGTTGAGGAACTGGTCGGCTGGAGCGGTGCGGAAAGCCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 2:291353/143‑1 (MQ=255)
ccGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt > 2:80931/1‑133 (MQ=255)
ccGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt < 1:80931/133‑1 (MQ=255)
aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GGTGATGAAGGCATTCCGGGTCGCCTGTCGGGTGAAGACTCCGGGCGCGGTACCTTGTTCCACCGCCCCGCGGCGCTCCACAAGCAGGCGAACgg > 2:60098/1‑143 (MQ=255)
aCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCGACCGCCACGCCGTGATCCCCGACCAGGCTGACgggt > 2:739984/1‑141 (MQ=255)
gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCTCGTTG‑GTTGATGAAGTCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 1:156617/143‑1 (MQ=255)
|
CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTG‑GTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760346‑760621
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
