Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A29 F18 I0 R1
|
22 |
15.5 |
612066 |
86.8% |
531273 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
100% |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 100.0%
| 19.3
/ ‑6.7
| 12 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/4); minor base C (1/0); total (7/5) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760360‑760611
|
atcgtcTGTAGGAGCTGGATAAACGAATCAGAACGGAGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 1:216657/138‑1 (MQ=255)
ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCGGTCTCGCGTTGCCAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAATACCTCCCttcggcct > 1:92727/1‑137 (MQ=255)
ctcgCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAACTGCAGTCTCGCGTTGCCAAAATATATGGCGATCGCCCGGCGATGACTGCCGGTGAGAAACTGTTAGACGAGCACGCGGCGGACAACCTCGCTGACGCAACgac > 2:224975/4‑141 (MQ=255)
cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACTTCGCTTACGCCGTGCTGGTTGATGAAg < 2:64607/143‑1 (MQ=255)
ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCggcccg > 2:277662/1‑139 (MQ=255)
gCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACCCCGGTCGCgggag > 1:304734/1‑140 (MQ=255)
ttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTACGGTCACGGTACCTTCTTcc > 1:187021/1‑143 (MQ=255)
aTGCCGATCGCCAGGCGATGTCTGTCGGTGATAATCTGTTCTATTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:75206/143‑1 (MQ=255)
cGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGTGTGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgc < 1:237406/143‑1 (MQ=255)
cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGTCCTTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:221739/1‑143 (MQ=255)
gCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt < 1:234556/143‑1 (MQ=255)
gaAAATGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGGCGCGGTACCGTCTTCCACCGCCACGCGGTGATCGACCACCAGTcaaccg > 2:242453/1‑138 (MQ=255)
|
AGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760360‑760611
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
