Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A29 F119 I1 R1
|
30 |
31.1 |
1326154 |
90.1% |
1194864 |
128.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 95.5%
| 44.0
/ ‑5.4
| 22 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (11/10); total (11/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 |
AGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAC > NC_000913/760369‑760601
|
aGGAGCTGGCGAAACGCTTCAGCACGGTAACAGAAAAAGAAGAAATGAAGTCTCGAGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa > 1:368210/1‑129 (MQ=255)
ctgcaggCGACACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGACTAGCGATACCAAGAATTATGGCAATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAacac > 1:212868/6‑129 (MQ=255)
gAAACGCATCAGCACGGTGCCGGAACCAGTTAAAATGCAATCAAGCGATGCAAAGAAAAATAGCGATCGCCAGGCGATGACTGCCGGTGAGAAACTGTTCGACTGGAGCAGTGCGGAAAACATCGCTTa > 1:566548/1‑129 (MQ=255)
ccGGACGCAGTTGACATGCAGTCTCGCTTTACCAAAAAATATGACAAAAAACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa > 1:69179/1‑129 (MQ=255)
cGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg < 1:52143/129‑1 (MQ=255)
aGTTGAAACGCAGCCTCGCGTTGCTAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTGGAGCCGTGCGGCAAACCTCGCTTACGCCACGCTGGTTGATTAACGCATTcc < 2:45339/129‑1 (MQ=255)
aGTCGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTTTATTTGTTTGTAAATTCTCGCTTACGCCACGCTGGTGGATGAAGGCATTcc < 1:493608/129‑1 (MQ=255)
gtacaTGCAGTCTCGCGTTGTCAAGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt < 2:555/125‑1 (MQ=255)
aaaTGCTGTCTCGCGTTGCCGAGTTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGATACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTc < 2:337456/129‑1 (MQ=255)
gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCAGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTc > 1:661207/1‑129 (MQ=255)
tctcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGACGGTGAGAAACTGTTCGACTGGAGCGGAGCGGAAAACCTCGCTTACGCAAAGCGGGTGGGTGAAGGCATTCCGGTACGccgggcg > 2:160645/1‑124 (MQ=255)
cgcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTAAGAAACTGATCGACAAGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGt > 1:330984/1‑129 (MQ=255)
aaGATTTATGGCCATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCTACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc < 2:512735/129‑1 (MQ=255)
aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCTACGCTGGGTGATGAAGGCATTGCGGTTCGCCCGTCGGGTGAAGACTCCGGTCGCGGTaacc > 2:57351/1‑126 (MQ=255)
aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCAACAAAAAAGAAACGGAAAAACTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTGGCGGTACct > 1:271284/1‑129 (MQ=255)
tCGCCAGGCGATGGCTGCCGGTGAGAAACTATTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACctt > 2:294377/1‑129 (MQ=255)
aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttcg > 1:268367/1‑128 (MQ=255)
gCGATGGCTGCCGGTGAGAAACTCTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTTCGGTTTGTTTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:331403/129‑1 (MQ=255)
cTGCCGGTGAGAAACTATTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGcgtgg < 1:294377/129‑5 (MQ=255)
gaAACTGTTCGACTGTAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCAACGCGGTGATCca < 2:661207/129‑1 (MQ=255)
cTGTTCGACTGGGGCGGTGCGGAAACCCTCGCTTACGCCTCGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCGTCCACCGCCACGCGGTGATCCACAAc < 2:222369/129‑1 (MQ=255)
cTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGTTTTATGATTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc < 1:194817/129‑1 (MQ=255)
|
AGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAC > NC_000913/760369‑760601
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
