breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 124,241 C→T Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 459,834 C→A A316E (GCG→GAG)  lon → Lon protease
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,440,791 C→T intergenic (‑7/‑267) ydbK ← / → ydbJ putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
RA 1,934,874 T→A T481S (ACC→TCC)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,634 A→C T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,440,147 G→A L295F (CTT→TTT)  rpoA ← RNA polymerase subunit alpha
RA 3,473,615 C→T W156* (TGG→TAG)  rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,711,031 G→A P326L (CCG→CTG)  yhjX ← putative pyruvate transporter
MC JC 3,815,859 Δ82 bp [rph] [rph]
JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,033,595 C→A P151T (CCT→ACT)  trkH → K(+) transporter TrkH
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 365810 366083 274 8 [7] [7] 8 lacZ beta‑galactosidase
* * ÷ NC_000913 366213 366416 204 8 [7] [7] 8 [lacZ] [lacZ]
* * ÷ NC_000913 1196270 1211430 15161 8 [7] [6] 8 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2818140 2818223 84 8 [6] [7] 8 [argY] [argY]
* * ÷ NC_000913 3423741–3424234 3424543–3424238 5–803 8 [7] [7] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 566777 =NA (NA)1114 (34.570) 229/252 0.0 97.6% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 28 (0.860)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein