Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F13 I0 R1 150 9496.0 7621532 98.4% 7499587 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,935,324 T→G 14.9% N705T (AAC→ACC)  lacZ ← beta‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,935,3240TG14.9% 245.8 / 12.3 140N705T (AAC→ACC) lacZbeta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (22/94);  new base G (21/3);  total (43/97)
Fisher's exact test for biased strand distribution p-value = 2.50e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CTCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935185‑3935463
                                                                                                                                            |                                                                                                                                           
cgcGATGCAAAAATCCGTTCCGCTGGTGGGCAGTTGAGGGATGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGGCGC‑Gt                                                                                                                                             <  5:240775/138‑1 (MQ=255)
 tCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGCTGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Gtt                                                                                                                                            <  8:458270/140‑1 (MQ=255)
    ctGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCGGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  8:8395/139‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGTGAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGGTTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  6:493419/140‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  1:596555/140‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  5:311730/140‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGGCACGCTGAGGTTTTCCGCCAGACGCCATTGCGGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTgg                                                                                                                                         <  6:379774/140‑1 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGt                                                                                                                                        >  1:522392/1‑140 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGt                                                                                                                                        >  2:438149/1‑140 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGt                                                                                                                                        >  1:888046/1‑140 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGGGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACACCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTTGt                                                                                                                                        >  5:122372/1‑140 (MQ=255)
        ataaaTCCTTTCGTCTGGTGTTCAGTTGGGGTATTGAGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCGAGACGCCATTGCTGGCAGGCGCTGATGTGTCCGTGTTCTGAGCTTGCTGTCGC‑GTTTTGTTGc                                                                                                                                     <  2:370323/138‑1 (MQ=255)
        aaaaaTCCGTTCCGCTGGGGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGc                                                                                                                                     <  5:24147/140‑1 (MQ=255)
          aaaTCCGTTCCGCTGTTTGGCAGTTGAGGGATGTAGTGGGACGCGGAGGGGAGCGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  3:79776/140‑1 (MQ=255)
          aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   >  3:508984/1‑140 (MQ=255)
          aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  5:1951/140‑1 (MQ=255)
          aaaTCCGTTCCGCTGGGGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  5:644849/140‑1 (MQ=255)
          aaaTCCGTACCGCTGGTGGTCAGTTGACGGATGTCGAGGGACGCGGAGGGGAGTTTCACGCTGAGGTTTTCCGCCAGACGCCTTTGGTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATTCGGTCGC‑GTGTGGTTGCAc                                                                                                                                   <  6:935321/140‑1 (MQ=255)
          aaaTCCGGTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  3:323967/140‑1 (MQ=255)
                ggtCCGCGGGTGGGCAGTGGAGGGATGGCGTGGGACGCGGAGGGGAGGGGCACGCTGAGGTTTTCCGCCAGACGCCATTGCGGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  3:347902/138‑1 (MQ=255)
                gTTCCGCTGGTGGTGCGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  1:705675/140‑1 (MQ=255)
                gTTCCGCTGGTGGTCAGTTGAGGGATGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  5:184571/140‑1 (MQ=255)
                gTTCCGCTGGTGGGCAGTTGCGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACTAcgcg                                                                                                                             <  5:66838/140‑1 (MQ=255)
                gTTCCGCTGGTGGGCAGTTGAGGGAGGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTGTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  1:928667/140‑1 (MQ=255)
                 ttCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGGAATACGCGt                                                                                                                            >  2:162654/1‑140 (MQ=255)
                  tCCGCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGGGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTa                                                                                                                           <  4:259966/140‑1 (MQ=255)
                     gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  4:421149/140‑1 (MQ=255)
                     gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGGGTCACGCTGAGGTGTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGGCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  6:464866/140‑1 (MQ=255)
                     gCTGGTGGGCCGTTGAGGGAGGGCGTGGGACGCGGAGGGGAGTGTCACGCGGAGGTTTTCCGCCAGACGCCATTGCTTCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  2:614118/140‑1 (MQ=255)
                     gCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCGGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTGGCACTACGCGTACCg                                                                                                                        <  1:74902/140‑1 (MQ=255)
                      cTGGTGGTCAGTTGAGGGATGGCGTGGGCCGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCATACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGGCCGACGCGTAcccg                                                                                                                       >  8:210506/1‑138 (MQ=255)
                          tggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGc                                                                                                                   <  3:105739/140‑1 (MQ=255)
                               aGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCagag                                                                                                              <  7:277613/140‑1 (MQ=255)
                                   gAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGGTGCACTAAGCGGACCGTTAGCCAGAGTTGt                                                                                                          >  8:239120/1‑140 (MQ=255)
                                    aGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTc                                                                                                         <  8:89973/140‑1 (MQ=255)
                                        aTGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTTCACTACGCGGACCCGTTTCCAGAGttttgccgg                                                                                                     >  3:281569/1‑133 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACGACGCGTACCGGTAGCCAGAGTTGTCCGgcg                                                                                                    >  8:177214/1‑140 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTTGTTGCCAAACGCGTAACGTTAGCCAGAGTTGTACggag                                                                                                    >  7:719869/1‑138 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTAACGGTAGCCCGAGTTGTACGgcg                                                                                                    >  5:852354/1‑140 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGGTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTgcggcg                                                                                                    >  4:602042/1‑140 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTGCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                    <  6:697365/140‑1 (MQ=255)
                                         tGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                    <  1:58735/140‑1 (MQ=255)
                                         gggCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCGATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                    <  1:555791/139‑1 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGct                                                                                                  >  4:436700/1‑140 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTTCACTACGCCTACCGTTAGCCAGAGGTTTCCGgcgcg                                                                                                  >  4:577577/1‑139 (MQ=255)
                                              tGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCAGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGGTAGCCAGACGTGTCCGGCGctcgc                                                                                               >  3:893368/1‑138 (MQ=255)
                                                   cgcgGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTTGACTAAGCGTCACCATATCCAGACGTGTCCGGGGCTCTCCCGcg                                                                                          >  3:913712/1‑139 (MQ=255)
                                                      ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGCCCATGCGGTCGC‑GTTTGGTTGCAATACGCGTACCGGTAGCCAGAGTTTTCCGGCGGTCTCCCgcggcg                                                                                       >  8:29224/1‑140 (MQ=255)
                                                      ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                       <  1:685450/140‑1 (MQ=255)
                                                      ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTTGTTGCACTACGCGGGCCGTTTGCCCGCGGTGTCCGGCGCTCACCgggggag                                                                                       >  2:305422/1‑135 (MQ=255)
                                                       gAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCgg                                                                                      <  8:499887/140‑1 (MQ=255)
                                                       gAGGGGAGTGGCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCgg                                                                                      <  2:739896/140‑1 (MQ=255)
                                                        aGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggc                                                                                     <  2:71283/140‑1 (MQ=255)
                                                           ggAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagt                                                                                  <  4:756902/140‑1 (MQ=255)
                                                                gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                             <  2:453134/140‑1 (MQ=255)
                                                                gtCACGCTGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                             <  8:441068/140‑1 (MQ=255)
                                                                ggcACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGAGGTGTACGGCTTCTGCCCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGGCCGGCGCGCTCCGGCTGCggcagatcagg                                                                             <  5:842490/138‑1 (MQ=255)
                                                                 tCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCCTCCCCGTAGCCAGAGTTGTCCGGCCCTCTACGGCTGCggcaggtaaatc                                                                            >  7:246879/1‑133 (MQ=255)
                                                                  cACGCTGAGGTTGTCCGCCAGAAGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTAACGGTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggca                                                                           <  8:356803/140‑1 (MQ=255)
                                                                     gCTGAGGTTTTCCTCCAGACGCCTTTGCTGGCAGGCGCCGATGTCTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTTCGCGTACCGTTAGCCAGAGGTGTCCGGCGCTCTCCTGCTGCggcagttcaggcagtt                                                                        <  6:702643/140‑1 (MQ=255)
                                                                     gCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggcagtt                                                                        <  4:612742/140‑1 (MQ=255)
                                                                     gCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggcagtt                                                                        <  8:336410/140‑1 (MQ=255)
                                                                     gCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggcagtt                                                                        <  5:543422/140‑1 (MQ=255)
                                                                     gCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggcagtt                                                                        <  4:261135/140‑1 (MQ=255)
                                                                      cTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTTGTTTCACTACGCGTACCGGTAGCCACAGTTTTCCGGCGCTCTCCGGGTGCgggagttaaggcagtta                                                                       >  4:717830/1‑139 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCAAGGCGCTGATGTGTCCGGCTTCTGCCCATGCGGTCGCGGTTTGGTT‑CACAACGCGTTCCGGTTGGCAGAGTTTTTCGGCACGAGACGGCAGCGGCAGGTGAGTCAGTttaat                                                                    >  5:117440/1‑140 (MQ=255)
                                                                          ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                   <  3:120332/140‑1 (MQ=255)
                                                                          ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                   <  5:369670/140‑1 (MQ=255)
                                                                           gTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatca                                                                  <  1:218560/140‑1 (MQ=255)
                                                                             tttCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAc                                                                <  5:934018/140‑1 (MQ=255)
                                                                                 cGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTTCt                                                            <  5:698814/140‑1 (MQ=255)
                                                                                 cGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCt                                                            <  2:420392/140‑1 (MQ=255)
                                                                                          ccATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCg                                                   <  2:403858/140‑1 (MQ=255)
                                                                                             ttGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTGCGGCGCTCTCCGGCTGCGGCCGTTAAAGCAGTTCAATAAACTGCTTACCATGCGGGc                                                >  2:156236/1‑140 (MQ=255)
                                                                                               gctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCa                                              <  1:423158/140‑1 (MQ=255)
                                                                                               gctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCa                                              <  3:780759/140‑1 (MQ=255)
                                                                                                ctgcCAGGCGCTGATGTGTCCGGGGTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCTCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCaa                                             <  7:581307/140‑1 (MQ=255)
                                                                                                   ccAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTCCGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCGGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCATGCGGGCCCACAt                                          >  2:567160/1‑140 (MQ=255)
                                                                                                       gcgcTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTGTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCag                                      <  1:351190/140‑1 (MQ=255)
                                                                                                          cTGGTGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACGACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCGGCGGCAGTTCAGGCTGTTCAATCACCAGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  3:555407/140‑1 (MQ=255)
                                                                                                          cTGATGTGTCCGGCTTCTGACCATGCTGTCGC‑GTGTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  8:144958/140‑1 (MQ=255)
                                                                                                          cTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  1:6949/140‑1 (MQ=255)
                                                                                                          cTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  3:964743/140‑1 (MQ=255)
                                                                                                                  tCCGGCTACTGACCATGCGGTCGC‑GGTTGTTTGCACTACGCGTACCGTTAGCCAGAGTTTTACGGCGCTCGACGGCTGCGGCAGTTACGGCACGTCAATCAACTGCGTACCGTTCGGGCCAACAACCCGCGGCACGTCaa                           >  4:763052/1‑139 (MQ=255)
                                                                                                                  gccGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCAc                           <  8:540356/139‑1 (MQ=255)
                                                                                                                              ccATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCg               <  4:117261/140‑1 (MQ=255)
                                                                                                                                 tGCGGTCGC‑GGTTGGTTTCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCGGCGGCAGTTAAGGGAGTTGAATCAACTGCTTAACGTGGGGGCCAACAACACGAGGCACTTCACCCCATGCGAGCGGcg            >  6:408800/1‑139 (MQ=255)
                                                                                                                                   cGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGGTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTg          <  5:390847/140‑1 (MQ=255)
                                                                                                                                     gTCGC‑GGTTGGTTGGACTACGCGGACCGTTAGCCAGAGTTGTCCGGCGCGGGCCGGCGGGGGCAGTTAAGGCAGGTAAATACACAGCGTAACGTGCGGGCCAACAACCCGAGGCCCTTCACCCCATGCCAGCCGCGTGcc        >  2:35279/1‑140 (MQ=255)
                                                                                                                                      tCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  1:266832/140‑1 (MQ=255)
                                                                                                                                      tCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCGCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  2:26180/140‑1 (MQ=255)
                                                                                                                                       cgc‑gGTTGGTTGCACTACGCGTACCGGTAGCCAGAGTTGTGCGGCGCTCGCCGGGTGCCGCAGTTAAGGCAGTTCAATCAACTGCGTACCATTCGGGCCCACATCCAGAGGCACTTCAACACTTGCCAGCGGCGTGccca      >  8:393433/1‑138 (MQ=255)
                                                                                                                                        gc‑gTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATc     <  7:142064/140‑1 (MQ=255)
                                                                                                                                         gggTTTGGTTGCACTACTCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTGCGGCTGAGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCGAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATc     <  6:212366/138‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTAGCGTTAGCGAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTAACTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGATTGCCATCCAg  <  7:665236/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  6:767873/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  3:481196/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  6:129906/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  6:118476/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  5:420239/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935185‑3935463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A