Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F35 I0 R1	195	10216.4	8585726	98.3%	8439768	135.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	3,935,324	T→G	9.7%	N705T (AAC→ACC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	3,935,324	0	T	G	9.7%	258.4 / 2.9	141	N705T (AAC→ACC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base T (30/92); new base G (11/6); total (42/99)
Fisher's exact test for biased strand distribution p-value = 1.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

ATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935189‑3935463

aTGCAAAAATCCGTTCCGCTGGTTGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCCGACCATGCGGTCGCGTTTgg                                                                                                                                            <  3:694344/140‑1 (MQ=255)

   cAAAACTCCGTTCCGCTGGTGGTAAGTTGAGGGATGGCGTGGGACGCGGATGGGAGTGTCACGCGGAGGTTTTACGCCAGACGCCATTGCTGCCAGGCGCTTATGTGTCAGGCTTCTGACCATGCGGTAGCGTTTTGt                                                                                                                                           >  6:712469/1‑138 (MQ=255)

     aaaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGTAGTGTCACTCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCAGGCTTCTGACCATGCGGTCGCGTTTGGTTg                                                                                                                                         <  6:88556/138‑1 (MQ=255)

     aaaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTg                                                                                                                                         <  5:278376/138‑1 (MQ=255)

     aaaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCa                                                                                                                                       <  2:1069554/140‑1 (MQ=255)

     aaaaTCCGTTCCGCTGGTGGGCAGTTGAGGGAGGGCGTGGGACGCGGAGGGGAGGGGCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCa                                                                                                                                       <  4:1031906/140‑1 (MQ=255)

     aaaaTCAGTTCCGCTGGTGGTCAGTTGCGGGATGGCGTGGGACTCGGAGGGGAGGTGCACGCTGAGGGTTTCAGCCAGACGCCATTGCTGCCAGTCGCTGATGTGTCAGGCTTCTGACCATGCGGTCGCGTTTGGTTGCa                                                                                                                                       <  2:1001136/140‑1 (MQ=255)

      aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCAc                                                                                                                                      <  3:775865/140‑1 (MQ=255)

      aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCAc                                                                                                                                      <  8:740971/140‑1 (MQ=255)

      aaaGCCGTTCCGCTGGTTGTCAGTGGTGGGATGGCGTGGGACGCGGAGGGGCGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTTCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGc                                                                                                                                        <  6:764578/138‑1 (MQ=255)

            gatCCGCTGGTGGTCAGTGGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTAcgcg                                                                                                                                <  7:950170/138‑1 (MQ=255)

            gTTCCGCGGGTGGTCAGTTGAGGGTTGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTAcg                                                                                                                                  <  6:723995/138‑1 (MQ=255)

                cGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTAcc                                                                                                                            <  3:701993/140‑1 (MQ=255)

                 gCTGGTGGTCAGTTGCGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTAc                                                                                                                             <  6:909486/138‑1 (MQ=255)

                 gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTAc                                                                                                                             <  5:75379/138‑1 (MQ=255)

                 gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCg                                                                                                                           <  4:1009377/140‑1 (MQ=255)

                 gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCg                                                                                                                           <  2:568290/140‑1 (MQ=255)

                 gCTGGTGGTCAGTTGAGGGAGGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCGTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTAc                                                                                                                             <  6:30363/138‑1 (MQ=255)

                 gCTGGGGGTCCGGTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCAGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCg                                                                                                                           <  8:584966/140‑1 (MQ=255)

                   tggtggTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGtt                                                                                                                         <  1:839539/140‑1 (MQ=255)

                      tggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGc                                                                                                                      <  3:488051/140‑1 (MQ=255)

                      tggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGc                                                                                                                      <  7:51902/140‑1 (MQ=255)

                      gggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTa                                                                                                                        <  5:936298/137‑1 (MQ=255)

                       ggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATAGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTTAACGACGCGGACCGATAg                                                                                                                       >  5:264920/1‑138 (MQ=255)

                        gtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCTCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTTGTTTTCCTACGGGGACCggtttcca                                                                                                                    >  7:40578/1‑133 (MQ=255)

                        gtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCCCTACGCGTTCCGTTAGCCa                                                                                                                    >  2:200077/1‑140 (MQ=255)

                          cAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTTCACTACGCGTAACGTTTGCCa                                                                                                                    >  6:143701/1‑138 (MQ=255)

                           tggtGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCagag                                                                                                                 <  4:392779/137‑1 (MQ=255)

                                agggagggCGGGGGACGCGGAGGGGAGGGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTg                                                                                                              <  5:523274/138‑1 (MQ=255)

                                   gATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCgg                                                                                                         <  4:579696/140‑1 (MQ=255)

                                     tGGCGTGGGACGCGGATGTGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTTTCCgg                                                                                                         <  6:848938/138‑1 (MQ=255)

                                     tGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGGCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                       <  3:290531/140‑1 (MQ=255)

                                     gggCGTTGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                       <  1:751889/139‑1 (MQ=255)

                                       gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGCCGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACAACGCGTACCGGTTGGCAGCGTTGTCCGgcgcg                                                                                                     >  3:922468/1‑139 (MQ=255)

                                         gTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcgcg                                                                                                     >  6:516952/1‑137 (MQ=255)

                                                 cgGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCCCTACGGCGACAGGTAGCCAGAGGTGTGCGGCGCGCTCCGGcggg                                                                                           >  2:44972/1‑137 (MQ=255)

                                                  ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGCCGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGCCCATGCGGTCGCGGTTGGTTGCACTACGCGTACCGGTTGAGAGACGTGTCAGGCGCGCTCCGGCTGCg                                                                                          >  8:599095/1‑140 (MQ=255)

                                                  ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                          <  7:82297/140‑1 (MQ=255)

                                                  ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGGACCGTTCGCCAGACTTGTCCGGCGCTCTCCCGCGGcc                                                                                          >  2:890265/1‑139 (MQ=255)

                                                  ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGGTGGACTACGCGTACCGTTAGCCAGAGTTGTCGCGCGCGCGCCCgcggcg                                                                                          >  8:491247/1‑140 (MQ=255)

                                                  ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCAGGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                          <  8:406000/140‑1 (MQ=255)

                                                       ggAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTTGTTGGACTACGGCTACCGTTAGGCCGAGTTTTACGGCGGGCGCCCGCGGCggcagg                                                                                     >  7:288475/1‑139 (MQ=255)

                                                         aGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGATTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttc                                                                                   >  2:291788/1‑140 (MQ=255)

                                                           tgtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCa                                                                                                                                       <  8:759987/86‑1 (MQ=255)

                                                           tgtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTGGTTGGCCTAAGCGGTCCGGTAGCCCGAGGTGTCCGGCGGTGTCCGGCGGGggctggtgat                                                                                 >  7:926239/1‑133 (MQ=255)

                                                            gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGTTTGGACTACGCGTAGCGTTTGGCAGGGTTTTGCGGGGCACTCCGGCGGCggcctttcagg                                                                                >  8:967902/1‑140 (MQ=255)

                                                            gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                                <  1:713125/140‑1 (MQ=255)

                                                            gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttca                                                                                  <  6:288917/138‑1 (MQ=255)

                                                            gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttca                                                                                  <  6:1001088/138‑1 (MQ=255)

                                                            gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTGTGGGTGGACTACGCGGACCGTTTGCCCGAGTTTTCCGGCGCTCGGCCGCTGGggcaggtgacg                                                                                >  3:408606/1‑134 (MQ=255)

                                                            gtCACGCTGAGGTCTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                                <  3:258286/140‑1 (MQ=255)

                                                             tCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTg                                                                                                                                         >  5:1038192/1‑82 (MQ=255)

                                                             tCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCAGATGTGTCCGGCTTCTGACCATGCGGTCTCGTTTTGTTGCACAACGCGTAACGATAGCAATAATTGTACGGCGCAAACCCGCAGCggcaagtaag                                                                                 >  6:546655/1‑132 (MQ=255)

                                                                     aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTTCACTACGCGGACCGTTAGCCAGAGGTGTCCGGCGCTCAACGGCGGCggcagttcaggcaggtcaca                                                                       >  2:851730/1‑138 (MQ=255)

                                                                     aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTGGTTGCACTACGCGTTCCGTTAGCCAGAGTTTTGCGGCGCGCTCCGGCTGCGGCAGTTCAGGGAGTtgaaa                                                                       >  7:754094/1‑139 (MQ=255)

                                                                     aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTAGCGTTTGGTTGCACTAAGCGTTCCGTTAGCCTGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTAAGGCAGTtcaat                                                                       <  7:761655/140‑1 (MQ=255)

                                                                      ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                      <  1:108274/140‑1 (MQ=255)

                                                                      ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                      <  1:978647/140‑1 (MQ=255)

                                                                      ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCAAGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                      <  7:478741/140‑1 (MQ=255)

                                                                          ttCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCAc                                                                                                                                      >  7:538731/1‑72 (MQ=255)

                                                                          ttCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCAc                                                                                                                                      <  8:538731/72‑1 (MQ=255)

                                                                            ccGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGc                                                               <  7:266722/140‑1 (MQ=255)

                                                                            ccGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGGTGCACTACGCGTACCGGTAGCCAGAGTTGTACTGCGCGATCCGGCTGCGGCAGTTAAGGCAGGTAAATAAAca                                                                  >  6:571761/1‑137 (MQ=255)

                                                                             cGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑t                                                             <  7:142394/140‑1 (MQ=255)

                                                                                    cGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTg                                                      <  2:746729/140‑1 (MQ=255)

                                                                                    cGCCATTGCTGCCAGGCGCTGATGTGGCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTg                                                      <  2:154269/140‑1 (MQ=255)

                                                                                       cATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCTTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGc                                                     <  5:49928/138‑1 (MQ=255)

                                                                                        aTTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCg‑gg                                                 <  4:349864/140‑1 (MQ=255)

                                                                                          tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTTCGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCATGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGcc                                               <  4:1017999/140‑1 (MQ=255)

                                                                                          tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGcc                                               <  3:841918/140‑1 (MQ=255)

                                                                                          tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCGCTCCGGCGGCGGCAGTTCAGGCCGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGcc                                               >  4:888004/1‑140 (MQ=255)

                                                                                          tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGGCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGcc                                               <  4:830678/140‑1 (MQ=255)

                                                                                          tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCATTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTGCAATCAAC‑TGC‑TTACCTTGCG‑GGcc                                               <  4:118053/140‑1 (MQ=255)

                                                                                           gctggCAGGCGCTGTTGTGTCGGGCTGGTGACCTTGCGGTCGCTTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGGAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCa                                              <  2:1092275/140‑1 (MQ=255)

                                                                                           gctgcCAGGCGCAGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCTCTCTCCGGCTGCGGAATTTCAGGCAGTTCAATCAAC‑TGA‑TTACCTTTCT‑GGCCa                                              <  8:428792/140‑1 (MQ=255)

                                                                                               ccAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTTGTTTCACTACGCGGACCGTTAGCCAGAGTTGTCCGGCGCGCGCCGGCGGCGGGAGTTGAGGGCGTTGAATCAAC‑TGCGTTCCGTTGGG‑GGccccca                                           >  4:542641/1‑136 (MQ=255)

                                                                                                 aGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGGCCAACGCGGACCGGTAGCCAGCGTTGTACCGGGCTCACCGGGGGCGGCAGTTAAAGCAGTTAAAAAAACATGT‑TTCCAATGCG‑GGCCacaacc                                         >  2:927086/1‑135 (MQ=255)

                                                                                                   gcgcTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTTGTTTCACTAAGCGTAACGTTAGCCATAGTTTTCAGGCGCGATCCGGGTGCGGCAGTTAAGGCCGTTCAATCAAC‑AGC‑TTAAAATGCG‑GGGCAAAAtac                                        >  6:387380/1‑136 (MQ=255)

                                                                                                   gcgcTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCag                                      <  4:822990/140‑1 (MQ=255)

                                                                                                     gcTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCagag                                    <  2:448917/140‑1 (MQ=255)

                                                                                                     gcTGAGGTGTCCGGCTTCTGACCATGCGGTGGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGGTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCTAC‑TTC‑TTACCTTGCG‑GGCCAACATCCagac                                    <  8:961090/140‑2 (MQ=255)

                                                                                                      cTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAgg                                   <  2:278939/140‑1 (MQ=255)

                                                                                                      cTGATGTGTCCGGCTTCTGACCATGCGGTCGCGGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAgg                                   <  2:175695/140‑1 (MQ=255)

                                                                                                      cTGATGTGTCCGGCTTCTGACCATGCGGTCGCGATTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAgg                                   <  1:291788/140‑1 (MQ=255)

                                                                                                           gtgtCCGGCTTCTGACGATGCGGTCGCGTTTGGTTGGACTAGGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCCGGCAGTTCAATCATC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACtt                              <  2:653779/140‑1 (MQ=255)

                                                                                                              tCCGGCTTCTGACCATGCGGTCGCGTTTTGTTTCACTAAGCGTTACGTTAGCCAGAATTGTCAGGCGCGCTCCGGCTGCGGCAGTTGAGGCAGTTAACTGAAA‑GTC‑TTACCATGAG‑GGCCCACAACACGGGGCACATCAc                           >  4:198056/1‑140 (MQ=255)

                                                                                                              tCCGGCTTCTGACCATGCGGTCGCGTTTGGGTGCACTACGCGTACCGGTAGCCAGTGTTTTCCGGCGCTCTCCGGCTGCGGCAGTTAAGGCAGTTAAATCAAC‑TGC‑TTAACATGCG‑GGCCAACATCCCCCGGCACTTCAc                           >  4:1014858/1‑140 (MQ=255)

                                                                                                                cGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCg                         <  2:265635/140‑1 (MQ=255)

                                                                                                                      cTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTg                     <  5:86771/138‑1 (MQ=255)

                                                                                                                          ccATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTGCCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCg               <  8:526021/140‑1 (MQ=255)

                                                                                                                            aTGCGGGCGCGGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGc             <  1:298703/140‑1 (MQ=255)

                                                                                                                             tGCGGTCGCGTTTGGTTTCCCTACGCGTACCGTTAGCCAGCGTTGTCCGGCGCGCGCCGGCTGCGGCCGTTAAGGCAGTTGAATCAAC‑TGC‑TTACCTTTCG‑GGCCCACAGCCCTCGGACCTTCACCCCTTTCCAGCGGcg            >  2:797221/1‑139 (MQ=255)

                                                                                                                             tGCGGTCGCGTTTGGTTGCACTACGCGAACCGTTATCCAAAGTATTACGGCGCTCTGCCGATGCCGCAGGACAGGCAGTTAAAAGAAC‑AGC‑TTACCATGCG‑GGCCAACCTCCTCAGGCACATCAACGCTGGCCAGCgggg            >  8:318885/1‑138 (MQ=255)

                                                                                                                             tGCGGTCGCGGTTTGTTGCACTACGCGTACCGGTAGCCAGAGTTGTCCGGCGCGCGCCGGCTGCGGCACGTGAGGGAGGTCAATCAAC‑ATG‑TTACCGTGCGTGGCCAAAAACC‑GAGGCACGTCCCCCCTTGGCAGCgggg            >  8:238076/1‑138 (MQ=255)

                                                                                                                               cGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCt            <  6:119125/138‑1 (MQ=255)

                                                                                                                               cGGGCGCGTTTGGTTGCACTACGGGTACCGTTAGCCAGAGTTGTCCGGCGCGCTCCGGCTGCGGCAGTACAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTg          <  4:967414/140‑1 (MQ=255)

                                                                                                                                  tCGCGTTTGGTTGCACTACGCGTTCCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTTCCAGCGGCTTGCCa       <  1:848716/140‑1 (MQ=255)

                                                                                                                                  tCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  2:933587/140‑1 (MQ=255)

                                                                                                                                       tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATcc    <  6:504847/138‑1 (MQ=255)

                                                                                                                                       tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  1:935688/140‑1 (MQ=255)

ATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGCGTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAAC‑TGC‑TTACCTTGCG‑GGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935189‑3935463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A