Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,935,324 T→G 11.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,935,3240TG11.2% 327.0 / 8.1 183N705T (AAC→ACC) lacZbeta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (46/111);  new base G (20/6);  total (66/117)
Fisher's exact test for biased strand distribution p-value = 8.45e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

CTCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935185‑3935463
                                                                                                                                            |                                                                                                                                           
cTCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGTACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCAAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Gt                                                                                                                                             >  8:874955/1‑140 (MQ=255)
 gcGATGCACACATCCGTTCCGATGGTGGGCAGTTGAGTGATGGCGTGGGACGCGGAGTGGAGGTTCACTCTGAGGGTTTCTGACCTACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Gtt                                                                                                                                            <  6:54270/139‑1 (MQ=255)
   gATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Gtttt                                                                                                                                          >  7:866179/1‑139 (MQ=255)
   gATGC‑AAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGCCGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  2:1081588/140‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Gttt                                                                                                                                           <  3:104940/138‑1 (MQ=255)
    aTGCAAAAATCCGTTCCGCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         <  8:713236/140‑1 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGCCGCCATTGCTGCCAGGCTCTGATGTGTCCGGCTTCTGACCAGGCGGTCGCGGTTTgg                                                                                                                                         >  8:671845/1‑140 (MQ=255)
     tGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGt                                                                                                                                        >  2:735220/1‑140 (MQ=255)
     ggCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGt                                                                                                                                        <  8:409366/139‑1 (MQ=255)
          aattCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGGCACGCGGAGGTTTTCCGCCAGACGCGATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  7:74825/137‑1 (MQ=255)
          aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑Ggttggttggcc                                                                                                                                   >  1:1081012/1‑137 (MQ=255)
          aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  5:13271/140‑1 (MQ=255)
          aaaTCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAc                                                                                                                                   <  1:191469/140‑1 (MQ=255)
             tCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCAATaa                                                                                                                                >  2:632978/1‑139 (MQ=255)
                gTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  7:34992/140‑1 (MQ=255)
                gTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcgcg                                                                                                                             <  5:478609/140‑1 (MQ=255)
                gTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTAcg                                                                                                                               <  4:824729/138‑1 (MQ=255)
                     gcgggtggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  2:956308/137‑1 (MQ=255)
                     gCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  2:412369/140‑1 (MQ=255)
                     gCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGGCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  7:940964/140‑1 (MQ=255)
                     gCTGGTGGGCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGGCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  5:835819/140‑1 (MQ=255)
                     gCTGGTGGGCAGTTGAGGGATGGCGGGGGACGCGGAGGGGAGTGTCACGCTGAGGTGTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCg                                                                                                                        <  2:592115/140‑1 (MQ=255)
                     gCTGGGGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTAc                                                                                                                          <  4:317004/138‑1 (MQ=255)
                       tggtgcTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGtt                                                                                                                      <  5:81124/140‑1 (MQ=255)
                          tggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTa                                                                                                                     <  3:500823/138‑1 (MQ=255)
                          tggtCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGc                                                                                                                   <  1:338127/140‑1 (MQ=255)
                              cAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGGACTACGCGTAACCTTATCCaga                                                                                                               >  5:374589/1‑140 (MQ=255)
                               aGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCagag                                                                                                              <  7:928124/140‑1 (MQ=255)
                                 ttGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGACCGGCTTCTGACCATGCGGTCTC‑GTGTGGTTTAACAACGCGTACCGTTAGCCAGAGtt                                                                                                            >  5:912699/1‑140 (MQ=255)
                                        aTGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTACg                                                                                                       >  4:239089/1‑138 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                    <  1:740607/140‑1 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGgcg                                                                                                    <  8:656089/140‑1 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGAGTACCCATAGCCAGAGTTTTACGgcg                                                                                                    >  2:467834/1‑140 (MQ=255)
                                         tGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTGCTGCCCATGCGGTCGC‑GTTTGGTTGGACTACGCGTACCGTTTTCCAGAGTTGTgcggcg                                                                                                    >  7:684293/1‑140 (MQ=255)
                                           gagTGGGACGCGGTGGGGAGTCTCACTCTGAGGTTTTCAGCCAGACGCCATTGCTGCCAGGCGCTGATGTCTACGTCATGTGACCATTCGGTAGC‑GTGTTGTTGAACTTAGCGTACCGTTAGCCACCGTTGTCCGGAGct                                                                                                  >  8:884142/3‑140 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTgg                                                                                                                                         >  8:535483/1‑101 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGGACTACGCGGACCGTTAGCCAGAGTTGTCCGgcgcg                                                                                                  >  6:123682/1‑139 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGGACCGTGAGCCAGAGTTTGCCGgcgcg                                                                                                  >  8:1015479/1‑139 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGACAGACGACATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTTCACTACGCGTACCGTTAGCCAGAGTTGTCCGGAGct                                                                                                  >  2:1039893/1‑140 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTATTCCGCCAGACGCCATTGCTGCCAGGCGCTGAGGTGTCCGGCTTCTGACCATGCGGTAGC‑GGGTGGTTGCGCGACGCGGACCCTGTGCCAGAGTTGGACGgcgag                                                                                                  >  2:377301/1‑138 (MQ=255)
                                           gCGTGGGACGCGGAGGGGAGTGTCAAGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCCTTAGCCAGAGTTGTCCCgcgca                                                                                                  >  8:189104/1‑139 (MQ=255)
                                             gggggACGCGGAGGGGAGGGTCACGCTGAGGCTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGGCGC‑GTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGctct                                                                                                <  7:545743/138‑1 (MQ=255)
                                             gTGGGCCGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCAGgcgcg                                                                                                  >  4:532784/1‑137 (MQ=255)
                                             gTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGGCCTACGCCTACCGTTAGCCAGAGTTGTCCGgggcg                                                                                                  >  4:1140046/1‑137 (MQ=255)
                                                ggACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACTACGCGGACCGTTAGCCCGAGTTGTCCCgcgccgtccc                                                                                             >  1:1000963/1‑134 (MQ=255)
                                                  aCGCGGAGGGGAGTGTCACGCTGAGGTTTTCCACCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTGCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGc                                                                                           <  5:10826/140‑1 (MQ=255)
                                                     cgGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCggcggc                                                                                        >  6:523708/1‑140 (MQ=255)
                                                      ggagtggagtGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTTCCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                       <  1:403949/140‑1 (MQ=255)
                                                      ggaggggaggGTCACGCGGAGGGTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTGCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                       <  8:512213/140‑1 (MQ=255)
                                                      ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                       <  7:838724/140‑1 (MQ=255)
                                                      ggAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCg                                                                                       <  2:1161312/140‑1 (MQ=255)
                                                       gAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGc                                                                                        >  4:348907/1‑138 (MQ=255)
                                                        aGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTTGTTGAACTAAGCGTACAGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggc                                                                                     >  6:470610/1‑140 (MQ=255)
                                                           ggAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGAGGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagt                                                                                  <  2:664763/140‑1 (MQ=255)
                                                               tgtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGCGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACAACGGGTACCGTTAGCCAGAGTTGTACGGCGCTCTCCCGCGGCggcagttcag                                                                              >  8:449169/1‑140 (MQ=255)
                                                                gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttca                                                                               <  3:348907/138‑1 (MQ=255)
                                                                gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttca                                                                               <  4:615316/138‑1 (MQ=255)
                                                                gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttca                                                                               <  3:334832/138‑1 (MQ=255)
                                                                gtCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCGTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                             <  1:1177383/140‑1 (MQ=255)
                                                                ggcACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTAGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcagg                                                                             <  7:479711/138‑1 (MQ=255)
                                                                 tCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCCGCGCTCTCCGGCTGCggccgttcag                                                                              >  4:924892/1‑138 (MQ=255)
                                                                    cGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTTGTTGCACTACGCCTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcaggtcatgcagt                                                                         >  5:692316/1‑140 (MQ=255)
                                                                      cTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGGACTACGCGTACCGTTATCCAGAGTTTTACGGCGCGGTCCGGGTGGggcaggtcaggccgttg                                                                       >  7:620106/1‑139 (MQ=255)
                                                                       tGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCCGCGCGCTCCGGCGGCggcagttaaggcagttca                                                                      >  6:148005/1‑140 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTTCTGCCAGGCGCTGATGGGGCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaat                                                                    <  7:147236/140‑1 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTTGGTGCACTAAGCGTACCGTTAGCCAGAGTTGGCCGGCGCTCTCCGGCGGGggcaggtgaggcagtggcaa                                                                    >  8:955577/1‑135 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCggcagttcaggcagttca                                                                      <  4:428340/138‑1 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCGGGGGCCGTTAAGGCAGTtcaaa                                                                    >  8:271482/1‑139 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTACAGACCATGCGGTCGC‑GGTTTGTTGCCCTACGCGTAACGGTAGCCAAAGTTTGACAGCCAAGACCAGCTGAggcagttcaggcaggagaat                                                                    >  8:582958/1‑134 (MQ=255)
                                                                         aGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGCGTACGGCTTCTGCCAATGCTGTCGC‑GGTTGGTTGCCCTACGCGTACCATTAGCCAGAGTTGTACGGCGATCGGCGGCGGCgggaggtctgggaggtaagt                                                                    >  2:1062285/1‑134 (MQ=255)
                                                                          ggTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                   <  6:432389/140‑1 (MQ=255)
                                                                           gTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTTCGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCCGCGGCGGCCGGTCAGGCCGGtcaat                                                                    >  3:89331/1‑138 (MQ=255)
                                                                           gTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatca                                                                  <  7:989382/140‑1 (MQ=255)
                                                                           gTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCGCTCCGGCTGCGGCAGTTCAGGCAGTtcaatca                                                                  <  6:702149/140‑1 (MQ=255)
                                                                           gTTTTCCGCCAGACGCCATAGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTTGTTTGACTAAGCGGACCGTTAGACACAGTTGTCCGGCGCGATCCGGCGTCGGCAGTTAAGGGAGTtgaaa                                                                    >  4:160930/1‑137 (MQ=255)
                                                                            ttttCCGCCAGACGCCAATGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTtcaatc                                                                   <  3:844716/138‑1 (MQ=255)
                                                                              ttCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTTGTTGGACTAAGCGTACCGGTTTCCAGAGTTGTCGGGCGCTCGCCGGGTGCGGGAGTTATCGGAGTTGAATCAAcg                                                               >  7:1071457/1‑139 (MQ=255)
                                                                                              tgctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCggg                                                 <  3:535338/138‑1 (MQ=255)
                                                                                               gctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCa                                              <  6:777219/140‑1 (MQ=255)
                                                                                               gctgcCAGGCGCTGATGGGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGGTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCa                                              <  8:116221/140‑1 (MQ=255)
                                                                                               gctgaCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCa                                              <  6:864176/140‑1 (MQ=255)
                                                                                                ctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCaa                                             <  5:16104/140‑1 (MQ=255)
                                                                                                ctgcCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCaa                                             <  8:1001298/140‑1 (MQ=255)
                                                                                                       gcgcTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCag                                      <  2:871357/140‑1 (MQ=255)
                                                                                                        cgcTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCaga                                     <  2:770097/140‑1 (MQ=255)
                                                                                                          cTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCCCTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  6:581724/140‑1 (MQ=255)
                                                                                                          cTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGAGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAgg                                   <  2:1110001/140‑1 (MQ=255)
                                                                                                                  tCCGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGGACTAAGCGTACCGGTTGCCACAGTTGTGCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGGTCAAAAAACTGCTTACCGTTGGGGCCAACATCCAGAGGCCCATCAc                           >  2:1007859/1‑140 (MQ=255)
                                                                                                                    cGGCTTCTGACCATGCGGTCGC‑GGTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCGCGGCGGCTGCGGCAGTTAAGGCAGTTAAAACAACTGCGTACCGTGCGGGCCAACATCCAGCGGCACTTCAc                           >  4:767952/1‑138 (MQ=255)
                                                                                                                              ccATGCGGTCGC‑GTTTGGGTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGGTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCg               <  2:198542/140‑1 (MQ=255)
                                                                                                                                aggCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCACCTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGc             <  6:367845/138‑1 (MQ=255)
                                                                                                                                 tGCGGTCGC‑GTTTGGTTTCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCGATCCCGCTGCGGCAGTTCAGGCAGTTCAATAAACTGCTTAACTTGCGGGGCAACATCCAGACGCACGTCACCGCGTGccccaggcg            >  6:972584/1‑133 (MQ=255)
                                                                                                                                   cGGGCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTg          <  8:318939/140‑1 (MQ=255)
                                                                                                                                      tCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  5:192855/140‑1 (MQ=255)
                                                                                                                                      tCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGATTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  6:7141/140‑1 (MQ=255)
                                                                                                                                        gc‑gTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCa       <  4:740773/138‑1 (MQ=255)
                                                                                                                                        gc‑gTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATc     <  5:257601/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGATGTGTCCGGCGCTCTCCTGCTGCTGCAGTTCAGGCAGTTAAATCAACTGCTTACCTTGCGGGACAACATACAGAGGCACTTCACCGCTAGCCAGCGGCTTGCCATCCAg  <  2:693796/140‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATcc    <  3:1125208/138‑1 (MQ=255)
                                                                                                                                            tttGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAg  <  6:1086840/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTCGATGCAAAAATCCGTTCCGCTGGTGGTCAGTTGAGGGATGGCGTGGGACGCGGAGGGGAGTGTCACGCTGAGGTTTTCCGCCAGACGCCATTGCTGCCAGGCGCTGATGTGTCCGGCTTCTGACCATGCGGTCGC‑GTTTGGTTGCACTACGCGTACCGTTAGCCAGAGTTGTCCGGCGCTCTCCGGCTGCGGCAGTTCAGGCAGTTCAATCAACTGCTTACCTTGCGGGCCAACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTGCCATCCAG  >  NZ_CP022686/3935185‑3935463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A