breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 214 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,4190CG73.2% 32.4 / 43.3 56I121M (ATC→ATGnohBbacteriophage DNA packaging protein
*NC_0009134,603,7761.G72.6% 180.4 / 78.4 84noncoding (80/87 nt)leuPtRNA‑Leu
*NC_0009131,949,4360AG71.9% 552.9 / 306.7 405L6L (CTA→CTGyecEhypothetical protein
*NC_0009131,634,0230AT71.0% 17.0 / 22.4 31G123G (GGT→GGAnohANohA
*NC_0009132,460,9001.C66.7% 209.2 / 117.1 99intergenic (+234/‑132)fadL/b2345FadL/hypothetical protein
*NC_0009131,949,4100CT66.1% 602.0 / inf 451L196L (CTC→CTTyecDhypothetical protein
*NC_0009131,949,4110AC66.1% 813.2 / inf 451N197H (AAC→CAC) yecDhypothetical protein
*NC_0009133,941,0900TC64.5% 48.9 / 109.4 110noncoding (34/76 nt)gltUtRNA‑Val
*NC_000913580,7830CA63.7% 264.5 / inf 477I69I (ATC→ATAybcXhypothetical protein
*NC_000913580,5090GC63.6% 41.0 / 75.4 66L151L (CTG→CTCnohBbacteriophage DNA packaging protein
*NC_0009131,634,0260TG62.9% 32.9 / 30.0 35A122A (GCA→GCCnohANohA
*NC_0009131,949,3560TC62.8% 314.6 / inf 411S178S (AGT→AGCyecDhypothetical protein
*NC_000913580,7770CT62.5% 504.5 / inf 466D67D (GAC→GATybcXhypothetical protein
*NC_0009131,949,3440GA62.4% 372.7 / inf 371Q174Q (CAG→CAAyecDhypothetical protein
*NC_000913580,7920GC62.4% 449.1 / inf 444V72V (GTG→GTCybcXhypothetical protein
*NC_0009131,949,4720GT62.4% 99.7 / inf 234G18G (GGG→GGTyecEhypothetical protein
*NC_000913580,7710CT62.3% 451.5 / inf 479G65G (GGC→GGTybcXhypothetical protein
*NC_000913580,7730GA62.2% 492.4 / inf 479S66N (AGC→AAC) ybcXhypothetical protein
*NC_000913580,7950TC62.1% 285.2 / inf 438N73N (AAT→AACybcXhypothetical protein
*NC_000913580,8010GT62.1% 201.7 / inf 430V75V (GTG→GTTybcXhypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 400839843 (0.240)36 (0.210)
+AAGA
17/172 8.7 46.4% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 43 (0.240)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 395825741 (0.230)19 (0.110)
+ACC
17/178 9.0 32.4% intergenic (‑826/‑35) ppiC/rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase
?NC_000913 3958258 = 41 (0.230)intergenic (‑827/‑34) ppiC/rep peptidyl‑prolyl cis‑trans isomerase C/rep helicase
* ? NC_000913 = 274373NA (NA)70 (0.390) 11/184 11.1 24.1% intergenic (‑32/‑176) trs51/ykfD IS5 transposase/putative amino acid/amine transport protein
?NC_000913 294583 = 221 (1.220)coding (221/441 nt) yagN hypothetical protein
* ? NC_000913 273179 =NA (NA)93 (0.510) 9/184 11.9 29.2% coding (1094/1131 nt) ykfC hypothetical protein
?NC_000913 = 294586 225 (1.240)coding (218/441 nt) yagN hypothetical protein
* ? NC_000913 294583 =221 (1.220)6 (0.030) 6/184 13.4 2.7% coding (221/441 nt) yagN hypothetical protein
?NC_000913 = 2065375 217 (1.200)intergenic (‑32/‑1255) trs56/b1995 IS5 transposase/hypothetical protein
* ? NC_000913 3088416 =207 (1.140)3 (0.020) 3/174 14.8 1.6% coding (51/708 nt) endA DNA‑specific endonuclease I
?NC_000913 3088433 = 184 (1.070)coding (68/708 nt) endA DNA‑specific endonuclease I
* ? NC_000913 3790742 =311 (1.710)3 (0.020) 3/176 14.9 1.0% coding (584/873 nt) htrL HtrL
?NC_000913 3790760 = 294 (1.700)coding (566/873 nt) htrL HtrL