breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 649,391 | T→G | I471L (ATC→CTC) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 743,241 | A→G | K310R (AAG→AGG) | sucB → | dihydrolipoamide acetyltransferase |
RA | 931,164 | T→G | E134A (GAA→GCA) | ybjE ← | predicted transporter |
RA | 1,014,635 | (T)7→8 | intergenic (‑32/‑324) | ssuE ← / → ycbQ | NAD(P)H‑dependent FMN reductase/predicted fimbrial‑like adhesin protein |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,609,130 | A→C | K285Q (AAG→CAG) | insF‑3 → | putative transposase for IS3 |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 1,822,155 | (A)8→7 | intergenic (‑56/+72) | ynjI ← / ← topB | predicted inner membrane protein/DNA topoisomerase III |
RA | 2,131,466 | (A)9→8 | intergenic (‑125/+175) | gatY ← / ← fbaB | D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,002,366 | G→T | A935S (GCT→TCT) | flu → | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
JC JC | 3,014,531 | IS150 (+) +3 bp | coding (1112‑1114/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
RA | 3,136,084 | A→G | intergenic (+121/+42) | cca → / ← uppP | fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase/undecaprenyl pyrophosphate phosphatase |
JC | 3,145,983 | Δ9 bp | coding (120‑128/1842 nt) | rpoD → | RNA polymerase sigma factor |
RA | 3,261,861 | G→A | F103F (TTC→TTT) | hflB ← | protease, ATP‑dependent zinc‑metallo |
RA | 3,339,403 | T→C | M81T (ATG→ACG) | fis → | DNA‑binding protein Fis |
RA | 3,464,820 | C→T | R150H (CGC→CAC) | ompR ← | osmolarity response regulator |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
MC JC | 3,843,787 | Δ54 bp | coding (251‑304/1404 nt) | dnaA ← | chromosomal replication initiation protein |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,991 | (T)5→4 | intergenic (‑192/‑131) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 4,163,752 | T→G | I966S (ATC→AGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,179,127 | C→A | N4K (AAC→AAA) | yjaG → | hypothetical protein |
RA | 4,201,960 | A→C | D200E (GAT→GAG) | iclR ← | DNA‑binding transcriptional repressor |
RA | 4,396,897 | A→T | I295N (ATC→AAC) | yjfR ← | predicted L‑ascorbate 6‑phosphate lactonase |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 761487–761513 | 761757–761646 | 134–271 | 55 [53] | [51] 58 | [lysT]–[lysW] | [lysT],valT,[lysW] |