breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 38 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.34.4% 226.8 / 126.9 98intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2930T.33.9% 259.8 / 149.3 116intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1613,7700CA33.8% 191.7 / 40.6 182intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,906,8320CA31.3% 137.2 / 11.9 98intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1936,2781.T30.6% 166.6 / 117.0 72intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,448,5260AC30.2% 588.0 / 100.3 362intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,169,5770TG29.9% 224.8 / 73.2 217R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,447,4030AC29.6% 538.5 / 90.5 325G346G (GGT→GGGmalZalpha‑glycosidase
*DH12,169,5480GT29.3% 375.5 / 72.7 227P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH12,326,2870GT27.3% 853.7 / 107.8 437intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH1936,2970AG27.2% 351.5 / 127.0 174intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,169,7200CG27.2% 196.5 / 45.0 160E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH1613,6130TG27.1% 184.0 / 34.5 201intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,591,5130TG26.5% 202.4 / 46.8 250D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH1102,7310AC25.4% 507.8 / 53.2 268intergenic (‑183/+15)trmL/lldDtRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/alpha‑hydroxy‑acid oxidizing enzyme
*DH1613,5970GC25.1% 281.1 / 42.1 234intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,448,5200AC25.1% 783.5 / 59.9 362intergenic (‑80/+76)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,965,9060TG24.5% 245.5 / 28.2 211intergenic (+139/‑356)aqpZ/ybjEaquaporin/transporter
*DH13,906,8530AT24.3% 197.3 / 33.7 111intergenic (+24/‑11)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1102,6180GC23.7% 615.3 / 55.9 236intergenic (‑70/+128)trmL/lldDtRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/alpha‑hydroxy‑acid oxidizing enzyme

Marginal new junction evidence (lowest skew 10 of 37 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 1355966 =187 (0.440)101 (0.250)
+CTTC
55/292 25.9 23.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 500 (1.190)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =187 (0.440)133 (0.320)
+CTTC
55/292 25.9 37.9% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 261 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =187 (0.440)207 (0.500)
+CTTC
55/292 25.9 53.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 = 1355505385 (0.910)58 (0.160) 32/260 33.0 18.3% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 184 (0.500)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355844 =126 (0.300)101 (0.240)
+C
38/298 34.2 34.4% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 261 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 3183985 =453 (1.070)72 (0.190)
+GATTGTTTCTTC
32/276 34.9 20.8% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 143 (0.340)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 3970042510 (1.210)34 (0.090) 31/282 36.2 6.6% intergenic (‑128/‑86) fimA/ECDH1_RS19620 type‑1 fimbrial protein, A chain/hypothetical protein
?DH1 = 3970338 476 (1.200)intergenic (+22/+58) ECDH1_RS19620/fimE hypothetical protein/tyrosine recombinase
* ? DH1 2326110 =NA (NA)44 (0.130)
+29 bp
20/242 38.2 NA intergenic (+30/+377) yddM/fdnI transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
?DH1 = 2326436 NA (NA)noncoding (148/176 nt) repeat_region repeat region
* ? DH1 = 2590941349 (0.830)69 (0.190) 23/260 38.5 24.0% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 135 (0.370)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 = 1519281302 (0.720)38 (0.100)
+AACCTTCTAATT
25/276 39.1 12.0% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 302 (0.720)coding (787/900 nt) yfaD transposase