breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 30 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,947,5910A.42.5% 147.0 / 122.3 73intergenic (+18/‑16)metW/metVtRNA‑Met/tRNA‑Met
*NC_0009131,708,9010AC33.0% 194.8 / 66.4 200R661R (CGA→CGCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_000913422,4290TG29.8% 581.7 / 96.8 344intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009132,947,4670AC28.1% 180.9 / 86.0 89intergenic (+4/‑30)metZ/metWtRNA‑Met/tRNA‑Met
*NC_0009131,552,1910CA28.0% 783.5 / 95.4 417intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
*NC_0009131,708,9100GC26.8% 347.3 / 31.5 193A664A (GCG→GCCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,818,1450TC26.1% 296.7 / 115.0 178intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
*NC_0009134,296,0600CT25.3% 630.5 / inf 487intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009133,269,9940GT25.0% 229.1 / 12.0 163intergenic (+392/+631)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_0009131,708,9300CA24.6% 369.1 / 39.7 198P671Q (CCG→CAG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009134,606,1920GC24.3% 124.4 / 10.1 76intergenic (‑27/+8)leuV/leuPtRNA‑Leu/tRNA‑Leu
*NC_0009131,308,7450TG23.9% 630.6 / 56.4 320intergenic (‑100/‑43)clsA/yciYcardiolipin synthase 1/uncharacterized protein
*NC_0009131,708,7580GC23.7% 143.3 / 31.1 122E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
*NC_0009132,818,1580TA23.2% 302.4 / 83.8 169intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
*NC_0009133,270,1670CG22.4% 296.3 / 32.6 196intergenic (+565/+458)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
*NC_000913422,4320TG22.3% 754.1 / 55.0 344intergenic (+73/‑86)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_000913915,2130AC22.2% 230.4 / 19.2 173intergenic (‑356/+139)ybjE/aqpZputative transporter/aquaporin Z
*NC_000913423,5520TG21.8% 576.9 / 41.3 262G345G (GGT→GGGmalZmaltodextrin glucosidase
*NC_000913422,4350TG21.8% 772.5 / 51.1 344intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
*NC_0009131,287,2170GT21.6% 720.8 / 121.9 351intergenic (‑41/+27)tpr/tyrVprotamine‑like protein/tRNA‑Tyr

Marginal new junction evidence (lowest skew 10 of 78 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =302 (0.810)63 (0.190) 57/266 15.6 18.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 288 (0.870)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805305 (0.820)57 (0.170) 52/266 17.2 17.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 281 (0.840)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 781369 =235 (0.630)147 (0.410)
+GAAG
55/290 18.2 42.9% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 168 (0.450)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781577 =266 (0.710)198 (0.550)
+GAAG
50/290 19.8 48.4% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 168 (0.450)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 780554 =386 (1.040)108 (0.300)
+GAAG
50/290 19.8 28.6% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521006 168 (0.450)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 1287084123 (0.330)77 (0.240) 37/258 21.9 31.7% intergenic (+558/+3) narI/tpr nitrate reductase 1, gamma (cytochrome b(NR)) subunit/protamine‑like protein
?NC_000913 1287557 = 225 (0.700)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 781446234 (0.630)58 (0.180) 35/258 22.7 19.2% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 2521487 = 285 (0.880)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB
* ? NC_000913 4542682 =452 (1.210)41 (0.120) 38/280 23.4 8.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 414 (1.180)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 696863 =114 (0.310)74 (0.220)
+GAAGAAACAATC
35/274 24.1 24.5% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 381 (1.020)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 2357690305 (0.820)56 (0.160)
+AATTAGAAGGTT
33/274 25.0 16.7% coding (787/900 nt) yfaD transposase_31 family protein
?NC_000913 2357691 = 305 (0.820)coding (788/900 nt) yfaD transposase_31 family protein