breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 224,846 T→C noncoding (1076/1542 nt) rrsH → 16S ribosomal RNA
RA 224,885 T→C noncoding (1115/1542 nt) rrsH → 16S ribosomal RNA
RA 224,889 C→T noncoding (1119/1542 nt) rrsH → 16S ribosomal RNA
RA 224,893 T→A noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA
RA 224,903 G→A noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA
RA 224,906 C→T noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA
RA 224,910 2 bp→GT noncoding (1140‑1141/1542 nt) rrsH → 16S ribosomal RNA
RA 224,915 A→C noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA
RA 224,920 A→T noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA
RA 224,923 G→A noncoding (1153/1542 nt) rrsH → 16S ribosomal RNA
RA 224,933 A→G noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA
RA 224,938 T→C noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA
RA 224,943 T→C noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA
RA 224,968 G→A noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA
RA 224,977 G→C noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA
RA 224,984 C→T noncoding (1214/1542 nt) rrsH → 16S ribosomal RNA
RA 224,989 A→T noncoding (1219/1542 nt) rrsH → 16S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 442,961 G→A E471E (GAG→GAA thiI → tRNA uridine 4‑sulfurtransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,649,867 T→G V25G (GTT→GGT)  ydfD → Qin prophage; lysis protein
RA 1,802,309 G→C P88A (CCG→GCG)  thrS ← threonine‑‑tRNA ligase
RA 1,863,500 A→G T244A (ACC→GCC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,616,162 C→A A23A (GCC→GCA bepA → beta‑barrel assembly‑enhancing protease
RA 2,653,971 T→G noncoding (117/304 nt) ryfA → small regulatory RNA RyfA
RA 2,886,861 T→C Y120C (TAT→TGT)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,426,967 T→C noncoding (67/76 nt) alaU ← tRNA‑Ala
RA 3,426,969 T→A noncoding (65/76 nt) alaU ← tRNA‑Ala
RA 3,426,985 A→T noncoding (49/76 nt) alaU ← tRNA‑Ala
RA 3,426,996 G→T noncoding (38/76 nt) alaU ← tRNA‑Ala
RA 3,427,002 A→G noncoding (32/76 nt) alaU ← tRNA‑Ala
RA 3,427,028 A→G noncoding (6/76 nt) alaU ← tRNA‑Ala
RA 3,427,034 A→T intergenic (‑1/+42) alaU ← / ← ileU tRNA‑Ala/tRNA‑Ile
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,768,355 C→T S60L (TCA→TTA)  yibG → tetratricopeptide‑like domain‑containing protein YibG
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,835,418 T→C N171S (AAT→AGT)  yicJ ← putative xyloside transporter YicJ
RA 3,847,546 C→G G360A (GGC→GCC)  uhpC ← inner membrane protein sensing glucose‑6‑phosphate
RA 4,037,198 A→G noncoding (58/77 nt) ileT → tRNA‑Ile
RA 4,037,207 C→A noncoding (67/77 nt) ileT → tRNA‑Ile
RA 4,037,213 T→C noncoding (73/77 nt) ileT → tRNA‑Ile
JC 4,037,218 42 bp→30 bp intergenic (+1/‑1) ileT → / → alaT tRNA‑Ile/tRNA‑Ala
RA 4,037,265 T→C noncoding (6/76 nt) alaT → tRNA‑Ala
RA 4,037,291 T→C noncoding (32/76 nt) alaT → tRNA‑Ala
RA 4,037,297 C→A noncoding (38/76 nt) alaT → tRNA‑Ala
RA 4,037,308 T→A noncoding (49/76 nt) alaT → tRNA‑Ala
RA 4,037,324 A→T noncoding (65/76 nt) alaT → tRNA‑Ala
RA 4,037,326 A→G noncoding (67/76 nt) alaT → tRNA‑Ala
RA 4,279,622 T→G F382V (TTT→GTT)  ghxP → guanine/hypoxanthine transporter GhxP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433019 433103 85 5 [4] [4] 5 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 1196287 1211437 15151 5 [4] [3] 5 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1978503 1979270 768 15 [0] [0] 9 insB‑5–insA‑5 insB‑5,insA‑5
* * ÷ NC_000913 2818144 2818205 62 5 [3] [3] 5 [argY] [argY]
* * ÷ NC_000913 3423780–3424523 3424523 1–744 5 [4] [4] 6 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 78114333 (1.010)15 (0.510)
+TAAACACTTTTTAAC
8/246 1.7 41.7% noncoding (76/76 nt) valZ tRNA‑Val
?NC_000913 2947605 = 14 (0.430)intergenic (+32/‑2) metW/metV tRNA‑Met/tRNA‑Met
* ? NC_000913 = 12994980 (0.000)21 (0.660) 19/268 0.6 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)11 (0.340) 9/276 1.7 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
* ? NC_000913 = 19785020 (0.000)14 (0.450) 13/260 1.0 100% intergenic (‑305/+16) flhD/insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA‑5/uspC IS1 protein InsA/universal stress protein C
* ? NC_000913 1979271 =0 (0.000)9 (0.270) 8/276 1.9 100% intergenic (‑56/‑482) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 240653519 (0.580)27 (0.820) 24/276 0.3 60.7% coding (107/939 nt) lrhA DNA‑binding transcriptional dual regulator LrhA
?NC_000913 2406551 = 16 (0.490)coding (91/939 nt) lrhA DNA‑binding transcriptional dual regulator LrhA
* ? NC_000913 3363001 =52 (1.590)46 (1.410) 30/274 0.1 64.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 34270370 (0.000)22 (0.830)
+26 bp
11/224 1.0 100% intergenic (‑4/+39) alaU/ileU tRNA‑Ala/tRNA‑Ile
?NC_000913 3427076 = NA (NA)noncoding (77/77 nt) ileU tRNA‑Ile
* ? NC_000913 = 3653230NA (NA)60 (1.830) 39/276 0.0 93.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.120)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =32 (0.980)10 (0.330) 8/258 1.8 31.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 13 (0.420)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269032 (0.980)21 (0.690) 16/258 0.7 49.5% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 13 (0.420)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit