breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 57 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP009273 | 4,594,167 | 0 | C | T | 65.7% | ‑1.0 / 30.3 | 35 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
* | NZ_CP009273 | 933,398 | 0 | A | C | 64.3% | 6.7 / 12.2 | 14 | T191P (ACC→CCC) | lolA | outer membrane lipoprotein chaperone LolA |
* | NZ_CP009273 | 4,594,161 | 0 | C | T | 62.9% | 2.3 / 31.3 | 35 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
* | NZ_CP009273 | 1,318,440 | 0 | T | G | 62.5% | 1.9 / 11.0 | 16 | V29G (GTG→GGG) | yciO | L‑threonylcarbamoyladenylate synthase |
* | NZ_CP009273 | 3,387,038 | 0 | A | T | 61.5% | ‑2.4 / 12.6 | 13 | S860R (AGT→AGA) | yhdP | AsmA2 domain‑containing protein YhdP |
* | NZ_CP009273 | 2,380,341 | 0 | G | A | 59.1% | ‑2.0 / 18.1 | 22 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
* | NZ_CP009273 | 2,380,347 | 0 | T | A | 56.5% | 10.9 / 26.8 | 23 | intergenic (+37/‑66) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
* | NZ_CP009273 | 1,024,801 | 0 | G | C | 55.6% | 5.2 / 11.2 | 9 | R209G (CGT→GGT) | rlmI | 23S rRNA (cytosine(1962)‑C(5))‑methyltransferase RlmI |
* | NZ_CP009273 | 3,171,107 | 0 | C | A | 55.2% | 15.4 / 42.1 | 29 | E55* (GAG→TAG) | nudF | ADP‑ribose diphosphatase |
* | NZ_CP009273 | 3,806,432 | 0 | A | C | 54.8% | 29.8 / 38.6 | 31 | T114T (ACA→ACC) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
* | NZ_CP009273 | 4,612,067 | 0 | A | G | 54.5% | 18.1 / 41.5 | 33 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
* | NZ_CP009273 | 2,380,362 | 0 | C | T | 54.2% | 2.4 / 30.4 | 24 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
* | NZ_CP009273 | 353,601 | 0 | C | A | 53.8% | 2.7 / 15.6 | 13 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
* | NZ_CP009273 | 1,559,878 | 0 | T | G | 53.8% | 12.7 / 17.0 | 13 | N38T (AAC→ACC) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
* | NZ_CP009273 | 2,356,152 | 0 | C | A | 53.8% | 9.4 / 20.9 | 13 | A321S (GCC→TCC) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
* | NZ_CP009273 | 3,387,034 | 0 | T | G | 53.8% | 11.7 / 13.9 | 13 | T862P (ACG→CCG) | yhdP | AsmA2 domain‑containing protein YhdP |
* | NZ_CP009273 | 2,832,718 | 0 | T | G | 53.6% | 29.5 / 36.0 | 29 | intergenic (‑95/‑165) | ascG/ascF | DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC |
* | NZ_CP009273 | 1,202,802 | 0 | T | G | 53.3% | 8.6 / 17.4 | 15 | V145G (GTC→GGC) | ymfQ | YmfQ family protein |
* | NZ_CP009273 | 1,379,045 | 0 | T | G | 53.3% | 10.5 / 16.5 | 15 | G224G (GGT→GGG) | ycjX | YcjX family protein |
* | NZ_CP009273 | 1,620,602 | 0 | G | C | 53.3% | ‑0.1 / 11.0 | 15 | R346G (CGG→GGG) | dcp | peptidyl‑dipeptidase Dcp |
Marginal new junction evidence (lowest skew 10 of 13 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | = 3851565 | 52 (1.780) | 5 (0.200) | 5/144 | 1.4 | 10.8% | coding (200/1494 nt) | yidJ | sulfatase‑like hydrolase/transferase |
? | NZ_CP009273 | = 3851588 | 39 (1.580) | coding (177/1494 nt) | yidJ | sulfatase‑like hydrolase/transferase | |||||
* | ? | NZ_CP009273 | = 4382322 | 22 (0.750) | 5 (0.190) | 5/154 | 1.5 | 19.3% | noncoding (34/76 nt) | BW25113_RS21640 | tRNA‑Gly |
? | NZ_CP009273 | = 4382323 | 22 (0.830) | noncoding (35/76 nt) | BW25113_RS21640 | tRNA‑Gly | |||||
* | ? | NZ_CP009273 | 3614374 = | 6 (0.210) | 4 (0.150) | 4/154 | 1.8 | 18.9% | coding (1823/4236 nt) | rhsB | rhs element protein RhsB |
? | NZ_CP009273 | 3757417 = | 29 (1.100) | coding (1875/4134 nt) | rhsA | RHS element protein RhsA | |||||
* | ? | NZ_CP009273 | 4506708 = | 25 (0.860) | 3 (0.120) | 3/152 | 2.0 | 10.3% | coding (827/954 nt) | fecR | fec operon regulator FecR |
? | NZ_CP009273 | 4506742 = | 30 (1.150) | coding (793/954 nt) | fecR | fec operon regulator FecR | |||||
* | ? | NZ_CP009273 | 4399442 = | 39 (1.340) | 3 (0.120) | 3/150 | 2.0 | 8.0% | coding (351/732 nt) | rlmB | 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB |
? | NZ_CP009273 | 4399525 = | 35 (1.360) | coding (434/732 nt) | rlmB | 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB | |||||
* | ? | NZ_CP009273 | = 3824404 | 26 (0.890) | 3 (0.120) | 3/148 | 2.0 | 9.7% | coding (588/1710 nt) | yicH | AsmA family protein |
? | NZ_CP009273 | = 3824452 | 33 (1.300) | coding (636/1710 nt) | yicH | AsmA family protein | |||||
* | ? | NZ_CP009273 | 3423234 = | 45 (1.540) | 3 (0.120) | 3/148 | 2.0 | 8.6% | coding (149/258 nt) | yrdB | DUF1488 domain‑containing protein |
? | NZ_CP009273 | 3423267 = | 25 (0.990) | coding (116/258 nt) | yrdB | DUF1488 domain‑containing protein | |||||
* | ? | NZ_CP009273 | 3256491 = | 37 (1.270) | 3 (0.120) | 3/148 | 2.0 | 8.1% | coding (529/1209 nt) | tdcD | propionate kinase |
? | NZ_CP009273 | 3256545 = | 36 (1.420) | coding (475/1209 nt) | tdcD | propionate kinase | |||||
* | ? | NZ_CP009273 | 3209524 = | 19 (0.650) | 3 (0.120) | 3/150 | 2.0 | 12.8% | coding (327/765 nt) | nfeF | NADPH‑dependent ferric chelate reductase |
? | NZ_CP009273 | 3209606 = | 24 (0.930) | coding (245/765 nt) | nfeF | NADPH‑dependent ferric chelate reductase | |||||
* | ? | NZ_CP009273 | 4306903 = | 20 (0.690) | 3 (0.110) | 3/158 | 2.1 | 9.9% | coding (133/1137 nt) | phnM | alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase |
? | NZ_CP009273 | 4306956 = | 36 (1.330) | coding (80/1137 nt) | phnM | alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase |