breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 196,535 C→G 7.1% S99* (TCA→TGA)  cdaA → diadenylate cyclase CdaA
RA 240,174 T→G 8.3% N120H (AAC→CAC)  EO946_RS01360 ← hypothetical protein
RA 240,207 G→T 9.0% Q109K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 485,053 G→T 8.8% L445F (TTG→TTT kimA → potassium transporter KimA
RA 607,359 A→C 13.9% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,413 A→C 15.7% T86P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,592 A→C 5.9% E145D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,638 G→C 9.3% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 30.5% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,702 C→T 19.6% A182V (GCC→GTC)  EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 14.1% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 634,078 C→G 9.4% E565Q (GAA→CAA)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein
RA 814,356 T→G 16.6% H340Q (CAT→CAG nosA → nitric oxide synthase
RA 1,097,399 C→A 5.4% Q48K (CAA→AAA)  EO946_RS05675 → MarR family transcriptional regulator
RA 1,152,044 G→T 8.1% D53E (GAC→GAA EO946_RS05920 ← GNAT family N‑acetyltransferase
RA 1,366,028 A→C 9.9% T176P (ACG→CCG)  EO946_RS07135 → PepSY domain‑containing protein
RA 1,514,854 T→G 10.9% intergenic (+58/+1) EO946_RS07915 → / ← EO946_RS07920 YlaH‑like family protein/YlaI family protein
RA 1,743,439 G→T 5.7% L167L (CTG→CTT cotE → outer spore coat protein CotE
RA 1,753,598 G→T 13.6% intergenic (+289/‑216) fabD → / → EO946_RS09090 ACP S‑malonyltransferase/acyltransferase domain‑containing protein
RA 1,805,539 G→A 6.2% T773T (ACG→ACA EO946_RS09140 → non‑ribosomal peptide synthetase
RA 1,805,557 C→T 5.7% V779V (GTC→GTT EO946_RS09140 → non‑ribosomal peptide synthetase
RA 1,805,565 T→C 6.1% M782T (ATG→ACG)  EO946_RS09140 → non‑ribosomal peptide synthetase
RA 1,805,596 A→G 6.0% K792K (AAA→AAG EO946_RS09140 → non‑ribosomal peptide synthetase
RA 1,805,602 G→C 6.5% V794V (GTG→GTC EO946_RS09140 → non‑ribosomal peptide synthetase
RA 1,805,603 T→C 6.1% S795P (TCG→CCG)  EO946_RS09140 → non‑ribosomal peptide synthetase
RA 2,034,593 C→A 17.4% intergenic (‑259/‑135) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein
RA 2,139,480 A→C 9.3% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase
RA 2,214,555 C→A 5.9% V48L (GTA→TTA)  nth ← endonuclease III
RA 2,502,242 T→G 19.9% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,502,244 T→G 11.5% H273P (CAC→CCC)  hrcA ← heat‑inducible transcriptional repressor HrcA
RA 2,729,917 C→A 10.3% K283N (AAG→AAT lcfA ← long‑chain‑fatty‑acid‑‑CoA ligase LcfA
RA 2,864,573 T→G 5.2% T415T (ACA→ACC pulA ← type I pullulanase
RA 2,982,236 T→C 41.7% noncoding (284/1550 nt) EO946_RS15435 ← 16S ribosomal RNA
RA 2,991,245 G→T 29.7% intergenic (+46/‑161) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 2,991,252 C→A 36.7% intergenic (+53/‑154) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 3,219,792 C→G 11.8% P377R (CCG→CGG)  oxdC → oxalate decarboxylase
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,285,567 C→T 32.0% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 32.1% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 34.7% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 36.6% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 28.5% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,294,907 T→A 6.0% D288V (GAT→GTT)  eno ← phosphopyruvate hydratase
RA 3,349,666 T→G 6.6% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase
RA 3,446,995 C→A 9.1% V197V (GTG→GTT degU ← two‑component system response regulator DegU
RA 3,447,038 T→G 12.8% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,539,589 T→G 7.5% I122L (ATC→CTC)  EO946_RS18215 ← T7SS effector LXG polymorphic toxin
RA 3,719,864 T→G 5.6% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein
RA 3,851,269 G→C 9.3% R36G (CGT→GGT)  EO946_RS19820 ← hypothetical protein
RA 3,906,067 A→C 12.4% I42S (ATT→AGT)  EO946_RS20100 ← DUF3255 family protein
RA 3,976,142 C→A 5.4% E22* (GAA→TAA)  rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
RA 3,996,857 C→A 8.6% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein
RA 3,996,927 C→A 6.6% M131I (ATG→ATT EO946_RS20530 ← VOC family protein
RA 4,000,117 G→T 7.5% L121F (TTG→TTT EO946_RS20555 → MFS transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)8 (0.030) 4/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
* ? NZ_CP034943 607695 =116 (0.480)24 (0.110)
+AGTAGCCCCT
13/232 NT 11.0% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 306 (1.260)coding (689/1125 nt) EO946_RS03185 collagen‑like protein