breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 375,019 Δ1 bp 9.1% intergenic (+138/‑440) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,020 T→G 9.2% intergenic (+139/‑439) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,060 C→T 10.1% intergenic (+179/‑399) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,065 G→A 9.8% intergenic (+184/‑394) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,066 G→C 9.8% intergenic (+185/‑393) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,067 A→T 12.0% intergenic (+186/‑392) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,069 G→C 10.1% intergenic (+188/‑390) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,072 Δ1 bp 9.8% intergenic (+191/‑387) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,074 G→C 10.0% intergenic (+193/‑385) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,075 G→A 9.8% intergenic (+194/‑384) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,076 G→T 11.0% intergenic (+195/‑383) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,079 T→A 10.1% intergenic (+198/‑380) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,080 T→C 10.0% intergenic (+199/‑379) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,081 T→C 10.1% intergenic (+200/‑378) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,100:1 +C 9.1% intergenic (+219/‑359) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 375,101 T→A 9.2% intergenic (+220/‑358) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 579,331 A→G 7.3% F88F (TTT→TTC ybcV ← DLP12 prophage, uncharacterized protein
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,504,726:1 +G 8.0% coding (1010/1149 nt) insQ → IS609 transposase B
RA 1,909,451 A→G 7.2% F217L (TTT→CTT)  kdgR ← KDG regulon transcriptional repressor
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 3,050,410 A→T 6.2% S86T (TCG→ACG)  gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,959,651 G→A 7.2% R54R (CGC→CGT ppiC ← peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,992,437 T→G 19.3% L429* (TTA→TGA)  cyaA → adenylate cyclase
JC 3,992,468 Δ4 bp 16.8% coding (1317‑1320/2547 nt) cyaA → adenylate cyclase
MC JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,149,252 G→T 5.6% T338N (ACC→AAC)  eptC ← LPS heptose I phosphoethanolamine transferase
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2533767 2536350 2584 17 [15] [15] 16 [ptsH]–crr [ptsH], ptsI, crr
* * ÷ NC_000913 3423837–3424230 3424402–3424236 7–566 16 [15] [13] 16 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)43 (0.800) 41/484 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 120023338 (0.700)3 (0.060) 3/462 NT 8.1% coding (574/1128 nt) intE e14 prophage, putative integrase
?NC_000913 = 1200412 32 (0.620)coding (395/1128 nt) intE e14 prophage, putative integrase
* ? NC_000913 1207790 =14 (0.260)25 (0.500) 21/452 NT 62.4% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 17 (0.340)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780513 (0.240)21 (0.420) 19/452 NT 59.0% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 17 (0.340)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =0 (0.000)55 (1.020) 41/484 NT 100% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 129339060 (1.110)3 (0.060) 3/470 NT 4.9% intergenic (‑468/‑137) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1293504 57 (1.090)intergenic (‑582/‑23) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 168798731 (0.570)3 (0.060) 3/466 NT 6.3% coding (391/1647 nt) fumA fumarate hydratase (fumarase A), aerobic Class I
?NC_000913 = 1688218 60 (1.150)coding (160/1647 nt) fumA fumarate hydratase (fumarase A), aerobic Class I
* ? NC_000913 = 187727563 (1.170)3 (0.060) 3/468 NT 4.8% intergenic (+19/+3) yeaO/yoaF DUF488 family protein/DUF333 family outer membrane lipoprotein
?NC_000913 = 1877290 59 (1.130)coding (243/255 nt) yoaF DUF333 family outer membrane lipoprotein
* ? NC_000913 1917402 =75 (1.390)3 (0.060) 3/474 NT 4.0% coding (1145/1284 nt) yebS inner membrane PqiA domain protein
?NC_000913 1917764 = 71 (1.340)coding (255/2634 nt) yebT MCE domain protein
* ? NC_000913 1979486 =0 (0.000)42 (0.780) 31/484 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)51 (0.940) 37/484 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2192868 =46 (0.850)5 (0.100) 3/468 NT 10.1% intergenic (‑74/+189) yehE/mrp DUF2574 family protein/antiporter inner membrane protein
?NC_000913 2193250 = 45 (0.860)coding (917/1110 nt) mrp antiporter inner membrane protein
* ? NC_000913 = 269228357 (1.060)3 (0.060) 3/476 NT 5.1% coding (3259/3888 nt) purL phosphoribosylformyl‑glycineamide synthetase
?NC_000913 = 2692298 55 (1.040)coding (3244/3888 nt) purL phosphoribosylformyl‑glycineamide synthetase
* ? NC_000913 2887322 =47 (0.870)3 (0.060) 3/468 NT 7.0% intergenic (‑105/‑30) ygcB/sokX Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/sok‑related sRNA, function unknown
?NC_000913 2887733 = 34 (0.650)coding (578/735 nt) cysH phosphoadenosine phosphosulfate reductase, PAPS reductase, thioredoxin dependent
* ? NC_000913 = 306849041 (0.760)3 (0.060) 3/472 NT 7.1% coding (317/636 nt) argO arginine transporter
?NC_000913 = 3068684 38 (0.720)coding (123/636 nt) argO arginine transporter
* ? NC_000913 = 315435140 (0.740)3 (0.060) 3/466 NT 7.2% coding (866/957 nt) yqhC transcriptional activator of yqhD
?NC_000913 = 3154364 39 (0.750)coding (853/957 nt) yqhC transcriptional activator of yqhD