breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,907 A→C 7.5% intergenic (+14/+21) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 9,908 G→T 7.6% intergenic (+15/+20) mog → / ← satP molybdochelatase incorporating molybdenum into molybdopterin/succinate‑acetate transporter
RA 36,358 C→A 9.0% A489A (GCG→GCT caiC ← putative crotonobetaine/carnitine‑CoA ligase
RA 83,587 A→C 6.2% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 83,590 G→T 6.2% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 120,154 Δ1 bp 5.4% intergenic (+19/+24) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 120,158:1 +A 5.6% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 252,589 G→C 5.5% A97P (GCA→CCA)  yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
RA 252,594 G→C 5.5% W98C (TGG→TGC yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
RA 256,449 T→C 7.7% intergenic (+14/‑78) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,454 C→G 8.2% intergenic (+19/‑73) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
RA 256,459 G→A 8.2% intergenic (+24/‑68) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 289,256 G→C 6.7% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 289,257 T→A 7.4% intergenic (‑95/+44) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 292,302 C→G 6.5% intergenic (+71/+20) yagJ → / ← yagK CP4‑6 prophage; uncharacterized protein;Phage or Prophage Related/CP4‑6 prophage; conserved protein
RA 475,971 C→G 6.0% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 5.9% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 481,225 T→G 6.9% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 481,228 C→A 6.8% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 774,356 C→G 5.1% P16P (CCC→CCG ybgE → putative inner membrane protein in cydABX‑ybgE operon
RA 915,080 G→C 5.2% intergenic (‑223/+272) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 915,081 G→C 6.0% intergenic (‑224/+271) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 987,560 G→C 5.5% intergenic (‑578/+25) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 987,561 G→C 5.5% intergenic (‑579/+24) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,015,912 T→A 7.1% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,018,983 A→T 5.3% intergenic (+46/+30) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,018,986 A→T 5.2% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
RA 1,141,148 C→T 5.6% intergenic (+162/+34) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,141,150 G→A 6.2% intergenic (+164/+32) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,141,151 T→C 5.8% intergenic (+165/+31) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,141,153 A→G 5.9% intergenic (+167/+29) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,147,548 T→G 5.3% intergenic (+8/‑73) rpmF → / → plsX 50S ribosomal subunit protein L32/putative phosphate acyltransferase
RA 1,147,549 C→A 5.2% intergenic (+9/‑72) rpmF → / → plsX 50S ribosomal subunit protein L32/putative phosphate acyltransferase
RA 1,174,077 G→A 6.3% intergenic (‑113/+15) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,174,080 T→C 6.3% intergenic (‑116/+12) mfd ← / ← ycfT transcription‑repair coupling factor/inner membrane protein
RA 1,211,610 A→G 6.7% intergenic (+33/+70) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
RA 1,211,612 A→T 6.8% intergenic (+35/+68) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
RA 1,211,614 C→T 6.7% intergenic (+37/+66) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
JC JC 1,293,015 IS1 (–) +8 bp 7.5% intergenic (‑93/‑505) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
JC JC 1,293,091 IS5 (+) +4 bp 11.4% intergenic (‑169/‑433) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,311,830 A→G 6.5% intergenic (+22/+18) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,311,831 C→T 6.3% intergenic (+23/+17) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
RA 1,324,839 G→T 5.3% E32* (GAA→TAA)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,330,396:1 +T 6.0% intergenic (+15/+21) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,330,399 A→T 6.0% intergenic (+18/+18) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,330,402 Δ1 bp 5.9% intergenic (+21/+15) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
RA 1,344,033 A→G 5.5% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family
RA 1,344,036 G→C 5.6% T104S (ACT→AGT)  yciT ← global regulator of transcription; DeoR family
RA 1,344,039 C→T 5.8% R103Q (CGG→CAG)  yciT ← global regulator of transcription; DeoR family
RA 1,449,050 T→A 7.8% intergenic (+32/+26) feaB → / ← tynA phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
RA 1,603,724 A→C 5.4% T236P (ACC→CCC)  lsrC → autoinducer 2 import system permease protein
RA 1,603,735 G→T 5.3% G239G (GGG→GGT lsrC → autoinducer 2 import system permease protein
RA 1,608,704 A→G 5.0% S107P (TCG→CCG)  yneE ← bestrophin family putative inner membrane protein
RA 1,625,976 A→C 30.4% F469V (TTC→GTC)  dcp ← dipeptidyl carboxypeptidase II
RA 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,689,817 C→A 7.3% intergenic (+66/‑35) manA → / → ydgA mannose‑6‑phosphate isomerase/DUF945 family protein
RA 1,689,819 A→T 7.2% intergenic (+68/‑33) manA → / → ydgA mannose‑6‑phosphate isomerase/DUF945 family protein
RA 1,689,821 T→G 7.3% intergenic (+70/‑31) manA → / → ydgA mannose‑6‑phosphate isomerase/DUF945 family protein
RA 1,799,370 Δ1 bp 5.7% intergenic (‑100/+23) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
RA 1,799,374:1 +T 5.8% intergenic (‑104/+19) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
RA 1,865,682 G→C 7.7% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
RA 1,932,094 C→G 5.3% intergenic (+35/+21) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
RA 1,932,095 T→A 5.3% intergenic (+36/+20) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
RA 1,932,096 C→G 5.3% intergenic (+37/+19) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,985,112 C→G 6.9% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 2,020,838 T→C 8.7% V251A (GTG→GCG)  fliM → flagellar motor switching and energizing component
RA 2,020,841 G→A 9.3% R252H (CGC→CAC)  fliM → flagellar motor switching and energizing component
RA 2,028,158 G→C 5.3% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,159 T→A 5.1% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,028,160 G→C 5.3% intergenic (‑143/+28) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein
RA 2,032,336 G→C 5.9% intergenic (‑19/+48) dcm ← / ← yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
RA 2,044,729 T→A 5.1% intergenic (+105/‑209) asnT → / → yeeJ tRNA‑Asn/putative adhesin
RA 2,062,354 T→A 5.9% intergenic (+19/+37) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,355 T→G 5.9% intergenic (+20/+36) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,356 C→A 5.9% intergenic (+21/+35) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,062,357 T→A 5.9% intergenic (+22/+34) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
RA 2,081,688 A→T 5.1% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
RA 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,286,313 T→A 5.2% intergenic (+26/+77) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,315 C→G 5.2% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,305,085 A→G 5.3% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 5.4% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 5.4% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,314,800 A→C 5.1% Q438P (CAG→CCG) ‡ rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,314,801 G→T 5.0% Q438H (CAG→CAT) ‡ rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,364,995 T→G 6.8% intergenic (+16/+23) nudI → / ← ais nucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase
RA 2,364,998 C→A 6.4% intergenic (+19/+20) nudI → / ← ais nucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase
RA 2,462,677 A→T 5.1% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,462,678 T→A 5.4% intergenic (+32/‑334) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,561,205 A→T 6.0% intergenic (+307/‑163) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,561,206 A→T 5.6% intergenic (+308/‑162) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,653,367 G→A 5.4% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,653,372 T→C 5.1% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,704,304 A→G 6.3% intergenic (‑241/+31) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)
RA 2,704,306 A→T 6.2% intergenic (‑243/+29) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)
RA 2,704,308 C→T 6.6% intergenic (‑245/+27) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)
RA 2,725,630 Δ1 bp 88.3% coding (117/1299 nt) kgtP ← alpha‑ketoglutarate transporter
RA 2,731,073 A→T 7.8% noncoding (85/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon
RA 2,731,074 A→T 7.7% noncoding (84/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon
RA 2,775,093 G→A 5.7% intergenic (+72/‑452) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,801,056 A→T 5.1% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin
RA 2,838,222 A→T 6.6% intergenic (‑117/+32) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
RA 2,838,231 A→T 6.6% intergenic (‑126/+23) hydN ← / ← ascG formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
RA 2,873,915 A→G 5.2% H26H (CAT→CAC) ‡ cysC ← adenosine 5'‑phosphosulfate kinase
RA 2,873,917 G→C 5.7% H26D (CAT→GAT) ‡ cysC ← adenosine 5'‑phosphosulfate kinase
RA 2,873,919 C→T 5.2% G25D (GGT→GAT)  cysC ← adenosine 5'‑phosphosulfate kinase
RA 2,918,017 C→T 5.4% intergenic (+204/+28) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,019 G→T 5.2% intergenic (+206/+26) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,020 A→C 5.6% intergenic (+207/+25) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,918,022 A→G 6.2% intergenic (+209/+23) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/D‑glucarate dehydratase 1
RA 2,971,146 T→C 5.8% intergenic (+13/‑125) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein
RA 2,971,151 T→C 5.4% intergenic (+18/‑120) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein
RA 2,971,154 G→A 5.6% intergenic (+21/‑117) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein
RA 2,971,159 G→A 6.2% intergenic (+26/‑112) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein
RA 3,020,510 G→T 7.1% intergenic (+21/‑30) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,511 C→G 7.4% intergenic (+22/‑29) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,020,512 A→C 7.8% intergenic (+23/‑28) ssnA → / → ygfM putative chlorohydrolase/aminohydrolase/putative oxidoreductase
RA 3,061,817 G→C 6.3% W323S (TGG→TCG) ‡ scpA → methylmalonyl‑CoA mutase
RA 3,061,818 G→C 6.3% W323C (TGG→TGC) ‡ scpA → methylmalonyl‑CoA mutase
RA 3,156,590 C→A 6.6% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,591 T→G 6.6% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,160,640 G→C 5.3% T169T (ACC→ACG ygiQ ← Radical SAM superfamily protein
RA 3,171,863 A→G 5.0% intergenic (+30/+16) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,180,505 G→T 6.1% E29* (GAG→TAG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,180,506 A→C 6.6% E29A (GAG→GCG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,183,345:1 +T 5.9% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,183,350 Δ1 bp 5.8% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,239,609 T→G 5.7% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,256,442 G→C 6.6% G70G (GGC→GGG yhaM ← putative L‑serine dehydratase alpha chain
RA 3,328,182 A→T 6.3% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,183 T→A 6.1% intergenic (+100/+56) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,184 A→T 5.8% intergenic (+101/+55) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,368,982 G→C 5.9% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
RA 3,407,293 T→A 6.1% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,407,294 T→A 6.0% intergenic (+28/‑81) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,407,295 T→A 6.0% intergenic (+29/‑80) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,418,628 G→A 6.0% intergenic (+17/‑414) yhdV → / → yhdW putative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
RA 3,418,629 T→C 6.1% intergenic (+18/‑413) yhdV → / → yhdW putative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
RA 3,423,229 T→G 5.9% intergenic (+35/+194) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,423,230 T→A 5.9% intergenic (+36/+193) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,423,231 T→A 6.3% intergenic (+37/+192) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,423,232 C→A 5.8% intergenic (+38/+191) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,458,556 C→A 64.4% S73* (TCG→TAG)  gspE → general secretory pathway component, cryptic
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,642,358 T→A 6.1% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,642,361 T→A 6.1% intergenic (+30/+19) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,708,595 G→A 5.4% intergenic (‑890/+21) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,596 T→A 5.5% intergenic (‑891/+20) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,597 T→C 5.4% intergenic (‑892/+19) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,774,207 C→T 5.6% intergenic (+13/‑217) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,774,214 C→T 5.9% intergenic (+20/‑210) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,774,215 A→G 6.0% intergenic (+21/‑209) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,774,222 A→G 5.3% intergenic (+28/‑202) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
RA 3,781,051 C→G 7.2% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,790,300 A→T 6.0% intergenic (‑219/+20) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
RA 3,790,303 A→T 5.9% intergenic (‑222/+17) waaH ← / ← tdh LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
JC JC 3,922,629 IS5 (–) +4 bp 15.1% intergenic (‑189/+425) atpI ← / ← rsmG ATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
RA 3,959,515 T→A 6.2% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,127,252 A→G 5.5% intergenic (+27/+34) rpmE → / ← yiiX 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein
RA 4,291,512 G→A 5.7% M1M (GTG→ATG) †‡ nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
RA 4,291,513 T→C 5.3% M1A (GTG→GCG) †‡ nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
RA 4,296,060 C→T 19.7% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,307,294 A→T 5.2% L169Q (CTG→CAG)  alsK ← D‑allose kinase
RA 4,335,654 T→A 5.9% intergenic (‑64/+40) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter
RA 4,335,655 T→A 5.9% intergenic (‑65/+39) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter
RA 4,362,528 G→A 5.9% intergenic (‑175/+23) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,362,529 T→C 6.4% intergenic (‑176/+22) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe
RA 4,426,592 T→A 6.1% intergenic (+35/+36) rplI → / ← yjfZ 50S ribosomal subunit protein L9/uncharacterized protein
RA 4,479,012 A→T 5.8% intergenic (+123/+22) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,016 G→C 5.6% intergenic (+127/+18) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,020 A→T 5.6% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,520,500 Δ1 bp 9.8% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 9.1% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423750–3424554 3424554 1–805 96 [93] [93] 96 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 224891 =NA (NA)4 (0.020) 3/290 NT NA noncoding (1121/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 224923 = NA (NA)noncoding (1153/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 274038 =NA (NA)4 (0.020) 4/286 NT NA noncoding (1112/1195 nt) IS5 repeat region
?NC_000913 274083 = NA (NA)noncoding (1067/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)3 (0.020) 3/280 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 474269 =NA (NA)4 (0.020) 4/286 NT NA intergenic (+17/+32) amtB/tesB ammonium transporter/acyl‑CoA thioesterase 2
?NC_000913 474296 = NA (NA)intergenic (+44/+5) amtB/tesB ammonium transporter/acyl‑CoA thioesterase 2
* ? NC_000913 1207790 =95 (0.500)100 (0.590) 71/266 NT 55.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 78 (0.460)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780594 (0.490)86 (0.510) 71/266 NT 51.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 78 (0.460)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)142 (0.770) 101/290 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 139255264 (0.340)140 (0.740) 112/298 NT 68.6% coding (562/900 nt) pgrR murein peptide degradation regulator
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 3091097170 (0.890)9 (0.050) 7/280 NT 5.3% intergenic (+43/‑37) endA/rsmE DNA‑specific endonuclease I/16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
?NC_000913 = 3091103 162 (0.910)intergenic (+49/‑31) endA/rsmE DNA‑specific endonuclease I/16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
* ? NC_000913 3423376 =180 (0.950)17 (0.090) 9/294 NT 8.8% intergenic (+182/+47) yhdZ/rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
?NC_000913 = 4213162 173 (0.920)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein
* ? NC_000913 3619878 =NA (NA)9 (0.050) 7/280 NT 100% coding (687/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619892 = 0 (0.000)coding (701/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3721045 =228 (1.200)498 (2.640) 226/296 NT 69.0% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 222 (1.180)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28
* ? NC_000913 = 4168341141 (0.740)7 (0.040) 6/278 NT 5.2% intergenic (+141/‑31) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
?NC_000913 = 4168363 125 (0.700)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 4614608 =137 (0.720)8 (0.040) 7/284 NT 5.8% noncoding (352/388 nt) REP352 (repetitive extragenic palindromic) element; contains 8 REP sequences REP352 (repetitive extragenic palindromic) element; contains 8 REP sequences
?NC_000913 4614641 = 131 (0.720)noncoding (385/388 nt) REP352 (repetitive extragenic palindromic) element; contains 8 REP sequences REP352 (repetitive extragenic palindromic) element; contains 8 REP sequences