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breseq version 0.28.1 revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 33 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | DH1 | 2,466,962 | 0 | A | T | 67.2% | 584.8 / inf | 426 | I145F (ATC→TTC) | ttcA | tRNA 2‑thiocytidine(32) synthetase TtcA |
* | DH1 | 936,169 | 0 | T | . | 48.9% | 171.2 / 179.1 | 93 | intergenic (‑13/+21) | ECDH1_RS04615/ECDH1_RS04620 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,208,477 | 0 | T | C | 42.3% | 182.5 / inf | 361 | K101K (AAA→AAG) | ynfM | transporter |
* | DH1 | 2,169,577 | 0 | T | G | 37.5% | 178.4 / 83.4 | 240 | R661R (CGA→CGC) | rsxC | electron transporter RsxC |
* | DH1 | 2,169,548 | 0 | G | T | 32.0% | 360.1 / 83.1 | 240 | P671Q (CCG→CAG) | rsxC | electron transporter RsxC |
* | DH1 | 613,770 | 0 | C | A | 29.3% | 277.3 / 55.2 | 261 | intergenic (+631/+392) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
* | DH1 | 3,906,837 | 0 | C | G | 29.2% | 162.6 / 26.0 | 107 | intergenic (+8/‑27) | ECDH1_RS19315/ECDH1_RS19320 | tRNA‑Leu/tRNA‑Leu |
* | DH1 | 936,293 | 0 | T | . | 28.1% | 339.7 / 141.2 | 139 | intergenic (‑27/+7) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,326,287 | 0 | G | T | 28.1% | 761.1 / 92.6 | 404 | intergenic (+207/+200) | yddM/fdnI | transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit |
* | DH1 | 613,613 | 0 | T | G | 27.5% | 245.8 / 46.8 | 246 | intergenic (+474/+549) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
* | DH1 | 3,448,526 | 0 | A | C | 26.5% | 743.7 / 107.8 | 421 | intergenic (‑86/+70) | malZ/proY | alpha‑glycosidase/proline‑specific permease ProY |
* | DH1 | 4,122,124 | 0 | C | A | 26.0% | 434.0 / 21.5 | 297 | intergenic (‑15/+22) | ECDH1_RS20430/ECDH1_RS20435 | tRNA‑Gly/tRNA‑Gly |
* | DH1 | 2,965,899 | 0 | C | G | 25.5% | 359.5 / 69.2 | 204 | intergenic (+132/‑363) | aqpZ/ybjE | aquaporin/transporter |
* | DH1 | 3,184,585 | 0 | C | A | 25.4% | 382.6 / 59.3 | 181 | intergenic (+8/‑30) | ECDH1_RS15795/ECDH1_RS15800 | tRNA‑Gln/tRNA‑TERM |
* | DH1 | 613,601 | 0 | G | T | 24.8% | 425.0 / 27.2 | 286 | intergenic (+462/+561) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
* | DH1 | 1,065,605 | 0 | A | T | 24.7% | 307.3 / 101.2 | 194 | intergenic (+40/‑23) | ECDH1_RS05245/ECDH1_RS05250 | tRNA‑Arg/tRNA‑Arg |
* | DH1 | 936,297 | 0 | A | G | 23.6% | 427.1 / 128.9 | 203 | intergenic (‑31/+3) | ECDH1_RS04620/ECDH1_RS04625 | tRNA‑Met/tRNA‑Met |
* | DH1 | 2,570,932 | 0 | A | C | 23.4% | 634.2 / 56.7 | 326 | G23G (GGT→GGG) | yciY | hypothetical protein |
* | DH1 | 1,065,618 | 0 | A | G | 22.9% | 343.9 / 118.3 | 216 | intergenic (+53/‑10) | ECDH1_RS05245/ECDH1_RS05250 | tRNA‑Arg/tRNA‑Arg |
* | DH1 | 613,597 | 0 | G | C | 22.9% | 493.4 / 48.3 | 309 | intergenic (+458/+565) | garK/yhaC | glycerate 2‑kinase/pentapeptide repeats‑related protein |
Marginal new junction evidence (lowest skew 10 of 40 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | DH1 | 1355966 = | 188 (0.380) | 149 (0.310) +CTTC |
56/292 | 8.7 | 39.3% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3099750 | 285 (0.570) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 1355966 = | 188 (0.380) | 229 (0.470) +CTTC |
56/292 | 8.7 | 55.6% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3099542 | NA (NA) | noncoding (268/268 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1355966 = | 188 (0.380) | 104 (0.210) +CTTC |
55/292 | 8.8 | 22.9% | noncoding (76/76 nt) | ECDH1_RS06685 | tRNA‑Val |
? | DH1 | = 3100565 | 530 (1.070) | noncoding (1/76 nt) | ECDH1_RS15385 | tRNA‑Lys | |||||
* | ? | DH1 | = 2590941 | 363 (0.730) | 80 (0.190) | 38/260 | 10.5 | 26.1% | intergenic (+1/‑209) | ECDH1_RS12805/ECDH1_RS12810 | tRNA‑Tyr/tRNA‑Tyr |
? | DH1 | 2591414 = | 140 (0.330) | noncoding (199/555 nt) | repeat_region | repeat region | |||||
* | ? | DH1 | 1355844 = | 136 (0.270) | 102 (0.210) +C |
40/298 | 11.3 | 32.8% | intergenic (‑45/+2) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099750 | 285 (0.570) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | = 1355505 | 440 (0.880) | 73 (0.170) | 32/260 | 11.6 | 20.4% | noncoding (54/348 nt) | repeat_region | repeat region |
? | DH1 | 3099693 = | 190 (0.440) | noncoding (58/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 3183985 = | 462 (0.930) | 74 (0.160) +GATTGTTTCTTC |
33/276 | 11.9 | 20.7% | intergenic (+379/‑1) | asnB/ECDH1_RS15770 | asparagine synthetase B/tRNA‑Met |
? | DH1 | = 3184256 | 155 (0.310) | intergenic (+2/‑33) | ECDH1_RS15780/ECDH1_RS15785 | tRNA‑Gln/tRNA‑Gln | |||||
* | ? | DH1 | = 1519281 | 335 (0.670) | 46 (0.100) +AACCTTCTAATT |
32/276 | 12.1 | 13.0% | coding (788/900 nt) | yfaD | transposase |
? | DH1 | 1519282 = | 335 (0.670) | coding (787/900 nt) | yfaD | transposase | |||||
* | ? | DH1 | 1355843 = | 130 (0.260) | 145 (0.290) +C |
25/298 | 14.4 | 41.3% | intergenic (‑44/+3) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099750 | 285 (0.570) | noncoding (1/76 nt) | ECDH1_RS15360 | tRNA‑Lys | |||||
* | ? | DH1 | 1355843 = | 130 (0.260) | 224 (0.450) +C |
25/298 | 14.4 | 63.4% | intergenic (‑44/+3) | ECDH1_RS06675/ECDH1_RS06680 | tRNA‑Val/tRNA‑Val |
? | DH1 | = 3099542 | NA (NA) | noncoding (268/268 nt) | repeat_region | repeat region |