breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,466,9620AT67.2% 584.8 / inf 426I145F (ATC→TTC) ttcAtRNA 2‑thiocytidine(32) synthetase TtcA
*DH1936,1690T.48.9% 171.2 / 179.1 93intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH12,208,4770TC42.3% 182.5 / inf 361K101K (AAA→AAGynfMtransporter
*DH12,169,5770TG37.5% 178.4 / 83.4 240R661R (CGA→CGCrsxCelectron transporter RsxC
*DH12,169,5480GT32.0% 360.1 / 83.1 240P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1613,7700CA29.3% 277.3 / 55.2 261intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,906,8370CG29.2% 162.6 / 26.0 107intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1936,2930T.28.1% 339.7 / 141.2 139intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,326,2870GT28.1% 761.1 / 92.6 404intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH1613,6130TG27.5% 245.8 / 46.8 246intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,448,5260AC26.5% 743.7 / 107.8 421intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH14,122,1240CA26.0% 434.0 / 21.5 297intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH12,965,8990CG25.5% 359.5 / 69.2 204intergenic (+132/‑363)aqpZ/ybjEaquaporin/transporter
*DH13,184,5850CA25.4% 382.6 / 59.3 181intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH1613,6010GT24.8% 425.0 / 27.2 286intergenic (+462/+561)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH11,065,6050AT24.7% 307.3 / 101.2 194intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH1936,2970AG23.6% 427.1 / 128.9 203intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,570,9320AC23.4% 634.2 / 56.7 326G23G (GGT→GGGyciYhypothetical protein
*DH11,065,6180AG22.9% 343.9 / 118.3 216intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH1613,5970GC22.9% 493.4 / 48.3 309intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein

Marginal new junction evidence (lowest skew 10 of 40 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 1355966 =188 (0.380)149 (0.310)
+CTTC
56/292 8.7 39.3% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 285 (0.570)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =188 (0.380)229 (0.470)
+CTTC
56/292 8.7 55.6% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 1355966 =188 (0.380)104 (0.210)
+CTTC
55/292 8.8 22.9% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 530 (1.070)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 = 2590941363 (0.730)80 (0.190) 38/260 10.5 26.1% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 140 (0.330)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 1355844 =136 (0.270)102 (0.210)
+C
40/298 11.3 32.8% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 285 (0.570)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1355505440 (0.880)73 (0.170) 32/260 11.6 20.4% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 190 (0.440)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 3183985 =462 (0.930)74 (0.160)
+GATTGTTTCTTC
33/276 11.9 20.7% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 155 (0.310)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 1519281335 (0.670)46 (0.100)
+AACCTTCTAATT
32/276 12.1 13.0% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 335 (0.670)coding (787/900 nt) yfaD transposase
* ? DH1 1355843 =130 (0.260)145 (0.290)
+C
25/298 14.4 41.3% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 285 (0.570)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355843 =130 (0.260)224 (0.450)
+C
25/298 14.4 63.4% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region