breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 163,135 Δ95,041 bp hokAyhhJ 83 genes
RA 839,095 Δ1 bp coding (1001/1434 nt) bglA ← 6‑phospho‑beta‑glucosidase
RA 915,795 A→C Q156P (CAG→CCG)  ptsP → phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 1,299,401 G→T A389S (GCG→TCG)  aegA → protein AegA
RA 1,493,381 T→C L292L (TTA→TTG elaD ← deubiquitinase
RA 1,514,629 C→T intergenic (+88/‑132) yfaY → / → rhmR competence/damage‑inducible protein A/transcriptional regulator
RA 1,618,508 T→A I6N (ATT→AAT)  psuG → pseudouridine‑5'‑phosphate glycosidase
RA 1,709,744 G→T G4C (GGT→TGT)  yegR → hypothetical protein
RA 1,755,118 G→A G14G (GGG→GGA cpsG → phosphomannomutase
RA 1,768,973 C→T T168I (ACA→ATA)  wzxB → polisoprenol‑linked O‑antigen transporter
RA 1,773,985 (A)8→7 coding (440/1119 nt) wbbK → hypothetical protein
RA 1,779,915 G→A E54E (GAG→GAA wzzB → chain length determinant protein
RA 1,820,579 G→A L207F (CTT→TTT)  yeeN ← transcriptional regulator
RA 1,885,611 G→A W125* (TGG→TAG)  pgsA → CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
JC JC 1,971,027 repeat_region (+) +4 bp :: Δ3 bp coding (141‑144/144 nt) yobF → DUF2527 domain‑containing protein
RA 1,985,603 G→T D121Y (GAT→TAT)  yoaA → ATP‑dependent helicase
RA 2,019,390 T→A I381N (ATC→AAC)  ydjK → MFS sugar transporter membrane protein
RA 2,122,777 (T)5→4 coding (4/1413 nt) pykF ← pyruvate kinase I
RA 2,232,214 Δ1 bp intergenic (‑141/‑392) flxA ← / → relB protein FlxA/antitoxin RelB
RA 2,244,937 A→G intergenic (‑81/+236) pinQ ← / ← ydfK Qin prophage; site‑specific recombinase/cold‑shock protein
RA 2,371,554 C→A L48F (TTG→TTT ydcP ← peptidase
RA 2,404,019 +G coding (239/861 nt) ydbC ← oxidoreductase
RA 2,509,217 G→A S82L (TCA→TTA)  ycjM ← alpha amylase catalytic domain family protein
RA 3,154,902 A→T I274F (ATC→TTC)  kdpB → potassium‑transporting ATPase B chain
RA 3,297,355 A→T N28K (AAT→AAA appY ← transcriptional regulator
RA 3,668,924 (T)5→6 pseudogene (353/455 nt) lpxA ← hydroxymyristoyl‑ACP dehydratase
RA 3,701,765 Δ1 bp coding (2129/2244 nt) fhuA ← ferrichrome‑iron receptor
RA 3,781,762 T→C intergenic (‑19/+583) mraZ ← / ← cra division/cell wall cluster transcriptional repressor MraZ/catabolite repressor/activator
RA 3,795,806 A→G intergenic (+73/‑91) sgrT → / → tbpA transcriptional regulator SgrR/thiamine‑binding periplasmic protein
RA 3,871,936 G→A S51F (TCT→TTT)  yjtD ← RNA methyltransferase
JC 3,898,512 Δ19 bp intergenic (+163/+239) yjjW → / ← yjjV glycine radical enzyme activase/DNAse
RA 3,913,413 T→G intergenic (+79/‑175) yjjA → / → opgB hypothetical protein/phosphoglycerol transferase I
RA 3,935,805 C→A I255I (ATC→ATA mcrC → protein mcrC
RA 3,944,733 T→C I75T (ATT→ACT)  mdtM → multidrug resistance protein MdtM
RA 3,980,009 A→G *86* (TAA→TAG yjhX → hypothetical protein
RA 4,137,730 C→A intergenic (‑24/+152) sugE ← / ← ecnB quaternary ammonium compound‑resistance protein SugE/entericidin B
RA 4,173,457 T→G W169G (TGG→GGG)  melR → melibiose operon regulatory protein
RA 4,198,371 C→T Q337* (CAG→TAG)  phnM → alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
RA 4,199,771 T→G V98G (GTT→GGT)  phnP → phosphoribosyl 1,2‑cyclic phosphodiesterase
RA 4,204,741 C→A H381N (CAT→AAT)  alsA → D‑allose import ATP‑binding protein AlsA
RA 4,414,695 G→A A251T (GCA→ACA)  rhaT → l‑rhamnose‑proton symporter
RA 4,482,593 C→A E239D (GAG→GAT pepQ ← Xaa‑Pro dipeptidase
RA 4,547,561 C→G A165P (GCA→CCA)  rho ← transcription termination factor Rho

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875192 (0.390)194 (0.440) 131/268 1.9 52.0% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 187 (0.420)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =189 (0.420)193 (0.430) 130/268 2.0 50.7% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 3635058 =137 (0.280)415 (0.880) 207/284 0.1 77.1% coding (263/741 nt) dnaQ DNA polymerase III subunit epsilon
?DH1 = 3635099 117 (0.250)coding (222/741 nt) dnaQ DNA polymerase III subunit epsilon