breseq  version 0.30.1  revision 4379201f728c
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 900 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH13,513,0650GT46.4% 101.3 / 69.7 110L267F (TTG→TTTcynRtranscriptional regulator
*DH12,726,1660AC44.4% 181.4 / 87.1 127Y92D (TAC→GAC) pabC4‑amino‑4‑deoxychorismate lyase
*DH1677,3280GA42.2% 210.4 / 108.5 166intergenic (‑31/‑179)ttdA/ttdRtartrate dehydratase subunit alpha/transcriptional activator TtdR
*DH1899,3220TG41.3% 276.0 / 40.4 138G384G (GGT→GGGyqeFacetyl‑CoA acetyltransferase
*DH12,782,1580TC40.3% 216.5 / 28.8 139noncoding (72/88 nt)ECDH1_RS13860tRNA‑Ser
*DH12,716,3680GA39.3% 167.2 / 75.6 112L38F (CTC→TTC) thiKthiamine kinase
*DH13,513,0750AG39.1% 159.1 / 37.6 115K271E (AAA→GAA) cynRtranscriptional regulator
*DH13,571,9950TG39.1% 240.7 / 43.2 128Y659D (TAT→GAT) paoCPaoABC aldehyde oxidoreductase Moco‑containing subunit
*DH13,281,5430AC38.8% 313.5 / 31.9 152V288G (GTG→GGG) cusAcation efflux system protein CusA
*DH12,655,8820CT38.6% 203.3 / 78.1 145F13F (TTC→TTTymgDhypothetical protein
*DH11,455,5290TG38.1% 327.6 / 29.9 155Y40S (TAC→TCC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate epimerase
*DH11,550,5220AC37.8% 311.0 / 11.7 144G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*DH11,628,4350GT37.7% 256.0 / 79.5 146V37F (GTT→TTT) lysPlysine‑specific permease
*DH1440,4870TG37.4% 295.3 / 47.3 147V274G (GTA→GGA) secYprotein translocase subunit SecY
*DH14,181,7900TG37.1% 402.6 / 29.4 178V161G (GTG→GGG) basStwo‑component sensor histidine kinase
*DH12,255,6620CT36.7% 175.3 / 90.7 128intergenic (+144/+75)dgcZ/yneMdiguanylate cyclase/hypothetical protein
*DH1140,1060TG36.7% 303.3 / 30.4 139T309P (ACC→CCC) yiaK2,3‑diketo‑L‑gulonate reductase
*DH12,716,3620CA36.7% 243.0 / 53.1 120E40* (GAA→TAA) thiKthiamine kinase
*DH11,365,2030TG36.6% 372.6 / 20.5 165V313G (GTG→GGG) mntHdivalent metal cation transporter
*DH14,135,7790TG36.4% 340.3 / 24.5 160V48G (GTA→GGA) ampCBlaEC family class C beta‑lactamase

Marginal new junction evidence (lowest skew 10 of 22 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 266887587 (0.530)71 (0.490) 62/260 3.6 50.8% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 60 (0.410)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =70 (0.480)70 (0.480) 61/260 3.8 50.2% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 2567932155 (0.950)23 (0.140) 15/292 18.3 12.9% coding (657/720 nt) tonB protein TonB
?DH1 = 3851581 NA (NA)noncoding (769/769 nt) repeat_region repeat region
* ? DH1 = 2567931155 (0.950)18 (0.110) 14/290 18.7 10.4% coding (658/720 nt) tonB protein TonB
?DH1 2830573 = NA (NA)noncoding (4/773 nt) repeat_region repeat region
* ? DH1 = 3703414155 (0.950)12 (0.080) 12/282 19.4 8.0% coding (480/2244 nt) fhuA ferrichrome‑iron receptor
?DH1 3703558 = 126 (0.800)coding (336/2244 nt) fhuA ferrichrome‑iron receptor
* ? DH1 = 2567917158 (0.970)17 (0.100) 13/292 19.4 9.7% coding (672/720 nt) tonB protein TonB
?DH1 = 3971755 NA (NA)noncoding (768/770 nt) repeat_region repeat region
* ? DH1 = 1405090NA (NA)58 (0.390) 9/262 20.2 45.3% noncoding (242/242 nt) repeat_region repeat region
?DH1 2668875 = 70 (0.480)pseudogene (20/345 nt) pinE hypothetical protein
* ? DH1 = 2567917158 (0.970)13 (0.080) 10/292 21.1 7.6% coding (672/720 nt) tonB protein TonB
?DH1 3580753 = NA (NA)noncoding (3/976 nt) repeat_region repeat region
* ? DH1 = 2568185134 (0.820)12 (0.070) 9/292 21.8 8.2% coding (404/720 nt) tonB protein TonB
?DH1 3850814 = NA (NA)noncoding (2/769 nt) repeat_region repeat region
* ? DH1 = 2568185134 (0.820)11 (0.070) 9/292 21.8 7.6% coding (404/720 nt) tonB protein TonB
?DH1 3580753 = NA (NA)noncoding (3/976 nt) repeat_region repeat region