breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 164,581 A→C 21.4% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 659,205 A→T 5.1% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 5.1% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 5.0% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 696,325 G→T 5.1% intergenic (+49/+105) ubiF → / ← glnX 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase/tRNA‑Gln
RA 699,432 G→A 5.5% intergenic (‑255/+142) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 699,433 C→A 5.2% intergenic (‑256/+141) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 699,439 G→T 5.2% intergenic (‑262/+135) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 699,440 A→C 5.3% intergenic (‑263/+134) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 705,951 T→A 5.5% intergenic (+61/‑142) nagE → / → glnS N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase
RA 710,170 C→T 5.6% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 6.6% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 781,238 T→A 7.2% intergenic (+16/‑131) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,254 A→T 7.8% intergenic (+32/‑115) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,269 C→T 6.2% intergenic (+47/‑100) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 847,216 G→T 5.0% intergenic (‑97/+42) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit
RA 857,578 C→G 5.3% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 915,226 T→G 17.5% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 1,015,912 T→A 5.6% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
RA 1,194,645:1 +A 5.8% coding (307/654 nt) rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,194,650 G→A 8.1% P101L (CCG→CTG)  rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,194,653 T→C 8.0% Q100R (CAG→CGG)  rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,194,657 Δ1 bp 8.1% coding (295/654 nt) rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,224,239 A→T 5.4% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor
RA 1,324,837 A→C 8.8% Y31S (TAT→TCT) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,838 T→A 8.3% Y31* (TAT→TAA) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,324,839 G→T 8.9% E32* (GAA→TAA)  yciQ → enhancer of membrane protein expression; putative inner membrane protein
RA 1,351,228 G→C 8.4% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,450,603 T→C 6.1% K249K (AAA→AAG tynA ← tyramine oxidase, copper‑requiring
RA 1,466,336 C→G 5.3% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,345 T→C 6.5% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,438 T→G 5.0% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,562 G→A 7.7% pseudogene (1171/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 7.9% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,630 G→C 5.2% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,709 T→A 11.0% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,805 T→A 8.7% R629R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 6.7% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,868,859 Δ1 bp 5.7% intergenic (+17/‑96) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
RA 1,868,865:1 +G 5.6% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,234 C→A 7.2% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,304,522 G→T 5.4% intergenic (+129/+586) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,523 G→C 6.1% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,649 C→T 8.1% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,383,956 A→T 6.1% intergenic (+32/+39) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,383,959 A→T 6.3% intergenic (+35/+36) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
RA 2,411,263 A→C 11.9% A30A (GCT→GCG yfbS ← putative transporter
RA 2,414,689 C→G 6.6% intergenic (+17/‑58) ackA → / → pta acetate kinase A and propionate kinase 2/phosphate acetyltransferase
RA 2,470,427 C→A 21.2% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,470,441 C→A 12.6% D437E (GAC→GAA gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,801,056 A→T 5.7% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin
RA 2,817,916 T→G 9.5% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,866,487 G→C 6.3% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 2,866,488 G→C 6.7% intergenic (+23/+71) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor
RA 3,046,050 T→G 10.0% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,046,136 A→C 5.2% intergenic (‑235/+32) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,066,308 A→T 5.7% I289N (ATT→AAT) ‡ ygfI ← putative DNA‑binding transcriptional regulator
RA 3,066,309 T→A 5.7% I289F (ATT→TTT) ‡ ygfI ← putative DNA‑binding transcriptional regulator
RA 3,066,310 A→T 5.4% H288Q (CAT→CAA ygfI ← putative DNA‑binding transcriptional regulator
RA 3,180,497 Δ1 bp 6.2% coding (77/1161 nt) ygiC → ATP‑Grasp family ATPase
RA 3,180,505 G→T 7.6% E29* (GAG→TAG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,180,506 A→C 8.0% E29A (GAG→GCG) ‡ ygiC → ATP‑Grasp family ATPase
RA 3,180,513:1 +A 5.5% coding (93/1161 nt) ygiC → ATP‑Grasp family ATPase
RA 3,270,082 A→C 5.3% intergenic (+480/+134) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,143 C→T 5.5% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,317,576 G→T 5.9% A144E (GCG→GAG)  rimP ← ribosome maturation factor for 30S subunits
RA 3,324,952 A→G 8.0% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 7.6% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,956 C→T 7.3% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 7.2% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,392,201 C→G 7.1% intergenic (‑173/+257) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,570,884 T→C 6.5% T146A (ACG→GCG)  glgX ← glycogen debranching enzyme
RA 3,608,695 C→G 14.0% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,729,405 C→G 5.1% intergenic (‑34/+38) xylB ← / ← xylA xylulokinase/D‑xylose isomerase
RA 3,729,408 C→G 5.1% intergenic (‑37/+35) xylB ← / ← xylA xylulokinase/D‑xylose isomerase
RA 3,781,051 C→G 7.0% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,815,809 Δ1 bp 11.0% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,828,980 A→C 11.0% Q12H (CAA→CAC xanP → xanthine permease
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,891,502 C→G 7.4% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 6.6% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,506 C→G 6.2% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,953,432 C→T 5.5% intergenic (+19/‑46) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
RA 3,953,433 A→G 5.5% intergenic (+20/‑45) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
RA 3,953,436 C→T 5.5% intergenic (+23/‑42) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
RA 3,953,437 A→G 5.5% intergenic (+24/‑41) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
RA 3,959,457 T→G 6.9% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,481 C→G 5.6% intergenic (+36/+51) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,501 T→A 15.0% intergenic (+56/+31) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,515 T→A 11.6% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,184,810 G→T 42.5% G1189V (GGT→GTT)  rpoB → RNA polymerase, beta subunit
RA 4,187,214 A→T 6.0% D622V (GAC→GTC)  rpoC → RNA polymerase, beta prime subunit
RA 4,235,478 T→C 5.0% intergenic (+71/‑428) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
RA 4,296,060 C→T 32.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,366,854 T→G 7.3% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,366,855 C→A 6.9% intergenic (‑82/+36) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,470,951 A→C 6.7% intergenic (‑38/+35) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,470,952 T→C 7.0% intergenic (‑39/+34) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,470,957 G→A 6.2% intergenic (‑44/+29) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,470,958 G→T 6.2% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,479,012 A→T 8.3% intergenic (+123/+22) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,016 G→C 7.8% intergenic (+127/+18) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,479,020 A→T 7.8% intergenic (+131/+14) rraB → / ← yjgM protein inhibitor of RNase E/GNAT family putative N‑acetyltransferase
RA 4,549,932 T→A 6.2% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter
RA 4,614,378 A→G 5.9% intergenic (+118/+302) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,614,444 A→C 6.7% intergenic (+184/+236) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772987 774212 1226 82 [77] [79] 86 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037684 1039353 1670 81 [78] [80] 82 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 2403897 2404669 773 81 [80] [76] 83 nuoB–[nuoA] nuoB,[nuoA]
* * ÷ NC_000913 3423723–3424234 3424577–3424238 5–855 82 [77] [79] 82 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 489959 =NA (NA)6 (0.040) 5/264 NT NA noncoding (1/98 nt) RIP43 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP43 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3547917 = NA (NA)noncoding (31/87 nt) REP254 (repetitive extragenic palindromic) element; contains 2 REP sequences REP254 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 947028 =156 (0.910)7 (0.050) 6/250 NT 5.2% intergenic (+9/‑201) ycaD/ycaM putative MFS transporter, inner membrane protein/putative transporter
?NC_000913 947064 = 116 (0.750)intergenic (+45/‑165) ycaD/ycaM putative MFS transporter, inner membrane protein/putative transporter
* ? NC_000913 1207790 =114 (0.660)44 (0.290) 24/246 NT 31.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 91 (0.600)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805110 (0.640)80 (0.530) 50/246 NT 45.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 91 (0.600)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)133 (0.800) 83/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 2817942131 (0.760)21 (0.140) 16/238 NT 15.9% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 110 (0.750)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 3666697 =NA (NA)5 (0.030) 3/264 NT 100% coding (884/1401 nt) gadA glutamate decarboxylase A, PLP‑dependent
?NC_000913 3666726 = 0 (0.000)coding (855/1401 nt) gadA glutamate decarboxylase A, PLP‑dependent
* ? NC_000913 = 4606166143 (0.830)8 (0.050)
+TACCAT
7/266 NT 5.1% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 = 4606427 167 (0.970)intergenic (‑26/+242) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase, SAM‑dependent