breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 224,846 T→C noncoding (1076/1542 nt) rrsH → 16S ribosomal RNA
RA 224,851 A→G noncoding (1081/1542 nt) rrsH → 16S ribosomal RNA
RA 224,885 T→C noncoding (1115/1542 nt) rrsH → 16S ribosomal RNA
RA 224,889 C→T noncoding (1119/1542 nt) rrsH → 16S ribosomal RNA
RA 224,893 T→A noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA
RA 224,903 2 bp→AC noncoding (1133‑1134/1542 nt) rrsH → 16S ribosomal RNA
RA 224,906 C→T noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA
RA 224,910 2 bp→GT noncoding (1140‑1141/1542 nt) rrsH → 16S ribosomal RNA
RA 224,915 A→C noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA
RA 224,920 A→T noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA
RA 224,923 G→A noncoding (1153/1542 nt) rrsH → 16S ribosomal RNA
RA 224,933 A→G noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA
RA 224,938 T→C noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA
RA 224,943 T→C noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA
RA 224,968 G→A noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA
RA 224,977 G→C noncoding (1207/1542 nt) rrsH → 16S ribosomal RNA
RA 225,499 T→A intergenic (+42/‑1) ileV → / → alaV tRNA‑Ile/tRNA‑Ala
RA 225,505 T→C noncoding (6/76 nt) alaV → tRNA‑Ala
RA 225,531 T→C noncoding (32/76 nt) alaV → tRNA‑Ala
RA 225,537 C→A noncoding (38/76 nt) alaV → tRNA‑Ala
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 435,813 T→A D60E (GAT→GAA thiL → thiamine monophosphate kinase
RA 571,663 T→C Y70Y (TAT→TAC ybcM → DLP12 prophage; putative DNA‑binding transcriptional regulator
RA 583,505 A→T intergenic (+445/‑176) tfaX → / → appY DLP12 prophage; protein TfaX/DNA‑binding transcriptional activator AppY
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,052,605 T→G D253A (GAC→GCC)  yccM ← putative 4Fe‑4S membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,622,811 A→G intergenic (+70/+6) mgtS → / ← mgrR small protein MgtS/small regulatory RNA MgrR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,406,063 Δ1 bp coding (579/939 nt) lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,490,314 G→A A546V (GCA→GTA)  oxc ← oxalyl‑CoA decarboxylase
RA 2,494,424 T→C intergenic (‑22/‑274) yfdX ← / → ypdI protein YfdX/colanic acid synthesis putative lipoprotein YpdI
RA 2,626,944 C→T L665L (CTG→CTA pdeF ← cyclic di‑GMP phosphodiesterase PdeF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,037,198 A→G noncoding (58/77 nt) ileT → tRNA‑Ile
RA 4,037,207 C→A noncoding (67/77 nt) ileT → tRNA‑Ile
RA 4,037,213 T→C noncoding (73/77 nt) ileT → tRNA‑Ile
RA 4,039,467 G→A noncoding (1949/2905 nt) rrlA → 23S ribosomal RNA
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196286 1211433 15148 7 [6] [6] 7 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423787–3424523 3424523 1–737 7 [6] [4] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 225496 =7 (0.170)25 (0.760)
+26 bp
11/224 1.4 86.1% intergenic (+39/‑4) ileV/alaV tRNA‑Ile/tRNA‑Ala
?NC_000913 = 4037217 3 (0.070)noncoding (77/77 nt) ileT tRNA‑Ile
* ? NC_000913 225500 =8 (0.200)44 (1.380)
+30 bp
16/216 0.7 91.1% noncoding (1/76 nt) alaV tRNA‑Ala
?NC_000913 = 4037217 3 (0.070)noncoding (77/77 nt) ileT tRNA‑Ile
* ? NC_000913 = 78114352 (1.280)11 (0.300)
+TAAACACTTTTTAAC
7/246 2.4 28.2% noncoding (76/76 nt) valZ tRNA‑Val
?NC_000913 2947605 = 11 (0.270)intergenic (+32/‑2) metW/metV tRNA‑Met/tRNA‑Met
* ? NC_000913 3363001 =52 (1.280)59 (1.460) 38/274 0.1 68.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)34 (0.830) 26/276 0.4 89.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.100)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =22 (0.540)18 (0.470) 16/258 1.1 46.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 21 (0.550)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269022 (0.540)10 (0.260) 10/258 1.9 32.5% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 21 (0.550)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit