breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 276 shown, sorted by frequency from high to low) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,515,909 | 0 | A | C | 60.0% | 23.1 / 97.1 | 75 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 720,466 | 0 | G | C | 51.7% | 13.4 / 44.3 | 29 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 433 | 0 | T | G | 50.6% | 70.5 / 140.2 | 87 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 2,437,406 | 0 | G | T | 47.2% | 35.7 / 24.1 | 36 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 476,746 | 0 | T | G | 45.3% | 139.1 / 23.8 | 95 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
* | REL606 | 2,376,348 | 0 | T | G | 43.2% | 208.8 / 61.4 | 155 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 3,774,914 | 0 | C | G | 41.6% | 253.2 / 196.7 | 198 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 4,111,429 | 0 | T | G | 41.2% | 112.6 / 17.3 | 68 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 3,378,885 | 0 | T | G | 41.1% | 352.3 / 88.9 | 209 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 4,180,249 | 0 | T | G | 39.8% | 232.5 / 36.8 | 123 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 1,853,067 | 0 | A | C | 39.1% | 182.4 / 108.3 | 133 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 1,854,401 | 0 | A | C | 38.5% | 135.4 / 32.8 | 92 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,735,608 | 0 | G | C | 38.5% | 30.6 / 10.0 | 26 | A409P (GCC→CCC) | ascF | fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component |
* | REL606 | 2,073,523 | 0 | A | C | 37.7% | 216.6 / 58.1 | 122 | G432G (GGT→GGG) | asmA | predicted assembly protein |
* | REL606 | 2,889,584 | 0 | C | A | 37.4% | 155.9 / 82.6 | 91 | T114N (ACC→AAC) | xdhC | xanthine dehydrogenase, Fe‑S binding subunit |
* | REL606 | 3,229,011 | 0 | A | C | 37.1% | 145.2 / 78.7 | 106 | T126T (ACA→ACC) | yraM | hypothetical protein |
* | REL606 | 851,606 | 0 | G | A | 37.1% | 146.0 / 90.1 | 116 | S3F (TCT→TTT) | ybiU | hypothetical protein |
* | REL606 | 691,852 | 0 | T | G | 36.9% | 272.2 / 26.6 | 160 | intergenic (+17/‑33) | ybfM/ybfN | predicted outer membrane porin/predicted lipoprotein |
* | REL606 | 3,305,660 | 0 | A | C | 36.3% | 444.4 / 36.5 | 215 | intergenic (‑342/+53) | sspA/rpsI | stringent starvation protein A/30S ribosomal protein S9 |
* | REL606 | 3,217,434 | 0 | G | C | 36.2% | 60.5 / 37.6 | 69 | A76P (GCC→CCC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 2056287 | 206 (0.840) | 48 (0.210) +CTTA |
16/142 | 11.0 | 14.4% | intergenic (‑191/+2) | cpsG/cpsB | phosphomannomutase/mannose‑1‑phosphate guanyltransferase |
? | REL606 | 4274785 = | 399 (1.630) | intergenic (+58/+473) | gltP/yjcO | glutamate/aspartate:proton symporter/hypothetical protein | |||||
* | ? | REL606 | = 3203123 | 301 (1.230) | 16 (0.090) +22 bp |
9/106 | 11.6 | 7.2% | intergenic (+571/+431) | yhaC/garK | hypothetical protein/glycerate kinase I |
? | REL606 | 3203139 = | 289 (1.180) | intergenic (+587/+415) | yhaC/garK | hypothetical protein/glycerate kinase I | |||||
* | ? | REL606 | 2781178 = | 308 (1.260) | 3 (0.010) | 3/132 | 16.9 | 1.1% | coding (22/1272 nt) | ygcN | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
? | REL606 | = 2781174 | 261 (1.220) | coding (18/1272 nt) | ygcN | predicted oxidoreductase with FAD/NAD(P)‑binding domain | |||||
* | ? | REL606 | = 2036902 | 263 (1.080) | 4 (0.020) | 3/134 | 17.0 | 2.0% | coding (57/1113 nt) | wbbC | putative glycosyltransferase WbbC |
? | REL606 | = 2037269 | 164 (0.750) | coding (862/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 462516 = | 160 (0.660) | 3 (0.010) | 3/134 | 17.0 | 1.5% | coding (364/528 nt) | priC | primosomal replication protein N'' |
? | REL606 | = 684651 | 262 (1.200) | coding (196/1149 nt) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase | |||||
* | ? | REL606 | = 135986 | 231 (0.950) | 3 (0.010) | 3/134 | 17.0 | 1.4% | coding (1568/2598 nt) | acnB | aconitate hydratase |
? | REL606 | = 3060774 | 210 (0.960) | coding (409/1173 nt) | ECB_02854 | conserved hypothetical protein | |||||
* | ? | REL606 | = 799331 | 396 (1.620) | 4 (0.020) | 3/140 | 17.4 | 1.2% | intergenic (‑56/+728) | ECB_00739/ybhB | hypothetical protein/predicted kinase inhibitor |
? | REL606 | = 799578 | 285 (1.250) | intergenic (‑303/+481) | ECB_00739/ybhB | hypothetical protein/predicted kinase inhibitor | |||||
* | ? | REL606 | 4012408 = | 265 (1.090) | 7 (0.030) | 3/144 | 17.6 | 2.8% | coding (891/1452 nt) | trkH | potassium transporter |
? | REL606 | 4012660 = | 235 (1.000) | coding (1143/1452 nt) | trkH | potassium transporter |