breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 276 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC60.0% 23.1 / 97.1 75V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606720,4660GC51.7% 13.4 / 44.3 29A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6064330TG50.6% 70.5 / 140.2 87V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,437,4060GT47.2% 35.7 / 24.1 36D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL606476,7460TG45.3% 139.1 / 23.8 95Q6H (CAA→CACfsrpredicted fosmidomycin efflux system
*REL6062,376,3480TG43.2% 208.8 / 61.4 155T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6063,774,9140CG41.6% 253.2 / 196.7 198A20P (GCC→CCC) yicJpredicted transporter
*REL6064,111,4290TG41.2% 112.6 / 17.3 68G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6063,378,8850TG41.1% 352.3 / 88.9 209T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL6064,180,2490TG39.8% 232.5 / 36.8 123G24G (GGT→GGGyjaHhypothetical protein
*REL6061,853,0670AC39.1% 182.4 / 108.3 133T56P (ACC→CCC) yeaNpredicted transporter
*REL6061,854,4010AC38.5% 135.4 / 32.8 92K88N (AAA→AACyeaOhypothetical protein
*REL6062,735,6080GC38.5% 30.6 / 10.0 26A409P (GCC→CCC) ascFfused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
*REL6062,073,5230AC37.7% 216.6 / 58.1 122G432G (GGT→GGGasmApredicted assembly protein
*REL6062,889,5840CA37.4% 155.9 / 82.6 91T114N (ACC→AAC) xdhCxanthine dehydrogenase, Fe‑S binding subunit
*REL6063,229,0110AC37.1% 145.2 / 78.7 106T126T (ACA→ACCyraMhypothetical protein
*REL606851,6060GA37.1% 146.0 / 90.1 116S3F (TCT→TTT) ybiUhypothetical protein
*REL606691,8520TG36.9% 272.2 / 26.6 160intergenic (+17/‑33)ybfM/ybfNpredicted outer membrane porin/predicted lipoprotein
*REL6063,305,6600AC36.3% 444.4 / 36.5 215intergenic (‑342/+53)sspA/rpsIstringent starvation protein A/30S ribosomal protein S9
*REL6063,217,4340GC36.2% 60.5 / 37.6 69A76P (GCC→CCC) agaStagatose‑6‑phosphate ketose/aldose isomerase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2056287206 (0.840)48 (0.210)
+CTTA
16/142 11.0 14.4% intergenic (‑191/+2) cpsG/cpsB phosphomannomutase/mannose‑1‑phosphate guanyltransferase
?REL606 4274785 = 399 (1.630)intergenic (+58/+473) gltP/yjcO glutamate/aspartate:proton symporter/hypothetical protein
* ? REL606 = 3203123301 (1.230)16 (0.090)
+22 bp
9/106 11.6 7.2% intergenic (+571/+431) yhaC/garK hypothetical protein/glycerate kinase I
?REL606 3203139 = 289 (1.180)intergenic (+587/+415) yhaC/garK hypothetical protein/glycerate kinase I
* ? REL606 2781178 =308 (1.260)3 (0.010) 3/132 16.9 1.1% coding (22/1272 nt) ygcN predicted oxidoreductase with FAD/NAD(P)‑binding domain
?REL606 = 2781174 261 (1.220)coding (18/1272 nt) ygcN predicted oxidoreductase with FAD/NAD(P)‑binding domain
* ? REL606 = 2036902263 (1.080)4 (0.020) 3/134 17.0 2.0% coding (57/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 = 2037269 164 (0.750)coding (862/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 462516 =160 (0.660)3 (0.010) 3/134 17.0 1.5% coding (364/528 nt) priC primosomal replication protein N''
?REL606 = 684651 262 (1.200)coding (196/1149 nt) nagA N‑acetylglucosamine‑6‑phosphate deacetylase
* ? REL606 = 135986231 (0.950)3 (0.010) 3/134 17.0 1.4% coding (1568/2598 nt) acnB aconitate hydratase
?REL606 = 3060774 210 (0.960)coding (409/1173 nt) ECB_02854 conserved hypothetical protein
* ? REL606 = 799331396 (1.620)4 (0.020) 3/140 17.4 1.2% intergenic (‑56/+728) ECB_00739/ybhB hypothetical protein/predicted kinase inhibitor
?REL606 = 799578 285 (1.250)intergenic (‑303/+481) ECB_00739/ybhB hypothetical protein/predicted kinase inhibitor
* ? REL606 4012408 =265 (1.090)7 (0.030) 3/144 17.6 2.8% coding (891/1452 nt) trkH potassium transporter
?REL606 4012660 = 235 (1.000)coding (1143/1452 nt) trkH potassium transporter