breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 4,195,874 | 0 | A | C | 42.2% | 68.2 / 19.6 | 45 | T318P (ACC→CCC) | aceB | malate synthase |
* | REL606 | 2,296,329 | 0 | A | C | 42.0% | 121.4 / 45.7 | 81 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 1,688,990 | 0 | A | C | 38.5% | 79.4 / 23.7 | 52 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 4,393,061 | 0 | T | G | 36.4% | 51.6 / 19.1 | 33 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 3,492,082 | 0 | T | G | 35.5% | 150.8 / 21.1 | 76 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 1,882,526 | 0 | T | G | 32.0% | 132.9 / 13.9 | 75 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 3,998,787 | 0 | A | C | 31.2% | 125.4 / 11.8 | 65 | T31P (ACC→CCC) | ubiB | putative ubiquinone biosynthesis protein UbiB |
* | REL606 | 1,484,105 | 0 | A | G | 30.8% | 69.0 / 14.2 | 39 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,875,483 | 0 | G | C | 28.1% | 290.2 / 126.4 | 139 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 3,983,700 | 0 | T | G | 25.0% | 90.8 / 12.6 | 40 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 4,169,232 | 0 | A | T | 23.6% | 132.1 / 12.3 | 55 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 3,691,844 | 0 | T | G | 23.2% | 136.0 / 10.9 | 56 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 1,747,903 | 0 | G | T | 22.7% | 286.1 / 59.5 | 97 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 1,802,326 | 0 | G | A | 21.7% | 127.4 / 10.0 | 46 | intergenic (‑124/+79) | ydjR/spy | hypothetical protein/envelope stress induced periplasmic protein |
Marginal new junction evidence (lowest skew 10 of 28 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 799199 = | 83 (1.280) | 7 (0.120) | 6/138 | 6.6 | 7.4% | coding (77/1146 nt) | ECB_00739 | hypothetical protein |
? | REL606 | 799388 = | 100 (1.680) | intergenic (‑113/+671) | ECB_00739/ybhB | hypothetical protein/predicted kinase inhibitor | |||||
* | ? | REL606 | 1219498 = | 70 (1.080) | 5 (0.080) | 5/138 | 7.1 | 6.9% | intergenic (+136/‑264) | ymgF/ycgH | hypothetical protein/predicted protein; b4491 |
? | REL606 | 1219720 = | 71 (1.190) | intergenic (+358/‑42) | ymgF/ycgH | hypothetical protein/predicted protein; b4491 | |||||
* | ? | REL606 | = 2678913 | 87 (1.340) | 7 (0.120) | 4/132 | 7.4 | 8.9% | intergenic (+267/‑16) | ECB_02509/ECB_02510 | Fels‑2 prophage protein/Recombinase |
? | REL606 | = 2679078 | 67 (1.180) | coding (150/1557 nt) | ECB_02510 | Recombinase | |||||
* | ? | REL606 | = 297897 | 125 (1.930) | 7 (0.120) | 4/136 | 7.5 | 6.2% | coding (117/669 nt) | ykgH | predicted inner membrane protein |
? | REL606 | = 298061 | 100 (1.700) | intergenic (‑48/+165) | ykgH/betA | predicted inner membrane protein/choline dehydrogenase | |||||
* | ? | REL606 | 281778 = | 71 (1.090) | 5 (0.090) | 4/134 | 7.5 | 6.9% | intergenic (‑261/‑514) | ykgK/ykgL | predicted regulator/hypothetical protein |
? | REL606 | 281954 = | 72 (1.240) | intergenic (‑437/‑338) | ykgK/ykgL | predicted regulator/hypothetical protein | |||||
* | ? | REL606 | 1519141 = | 66 (1.020) | 5 (0.080) | 4/138 | 7.6 | 6.9% | coding (725/957 nt) | yddK | hypothetical protein |
? | REL606 | 1519345 = | 74 (1.240) | coding (521/957 nt) | yddK | hypothetical protein | |||||
* | ? | REL606 | 2042256 = | 69 (1.060) | 5 (0.080) | 4/140 | 7.7 | 6.7% | coding (565/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2042511 = | 76 (1.260) | coding (310/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 2041682 = | 41 (0.630) | 3 (0.050) | 3/128 | 7.8 | 6.3% | coding (1139/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2041834 = | 55 (1.000) | coding (987/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 891259 | 102 (1.570) | 5 (0.080) | 4/142 | 7.8 | 5.2% | coding (282/963 nt) | ECB_00831 | protein similar to RNA‑directed DNA polymerase from retron EC86 |
? | REL606 | = 891492 | 85 (1.390) | coding (515/963 nt) | ECB_00831 | protein similar to RNA‑directed DNA polymerase from retron EC86 | |||||
* | ? | REL606 | = 2114182 | 60 (0.930) | 4 (0.060) | 4/144 | 7.9 | 8.0% | coding (492/1968 nt) | ECB_02001 | hypothetical protein |
? | REL606 | = 2114374 | 35 (0.560) | coding (300/1968 nt) | ECB_02001 | hypothetical protein |