breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,195,8740AC42.2% 68.2 / 19.6 45T318P (ACC→CCC) aceBmalate synthase
*REL6062,296,3290AC42.0% 121.4 / 45.7 81intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,688,9900AC38.5% 79.4 / 23.7 52T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6064,393,0610TG36.4% 51.6 / 19.1 33V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6063,492,0820TG35.5% 150.8 / 21.1 76V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,882,5260TG32.0% 132.9 / 13.9 75I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6063,998,7870AC31.2% 125.4 / 11.8 65T31P (ACC→CCC) ubiBputative ubiquinone biosynthesis protein UbiB
*REL6061,484,1050AG30.8% 69.0 / 14.2 39G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,875,4830GC28.1% 290.2 / 126.4 139intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6063,983,7000TG25.0% 90.8 / 12.6 40T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6064,169,2320AT23.6% 132.1 / 12.3 55intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6063,691,8440TG23.2% 136.0 / 10.9 56N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6061,747,9030GT22.7% 286.1 / 59.5 97A8S (GCA→TCA) ydiLhypothetical protein
*REL6061,802,3260GA21.7% 127.4 / 10.0 46intergenic (‑124/+79)ydjR/spyhypothetical protein/envelope stress induced periplasmic protein

Marginal new junction evidence (lowest skew 10 of 28 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 799199 =83 (1.280)7 (0.120) 6/138 6.6 7.4% coding (77/1146 nt) ECB_00739 hypothetical protein
?REL606 799388 = 100 (1.680)intergenic (‑113/+671) ECB_00739/ybhB hypothetical protein/predicted kinase inhibitor
* ? REL606 1219498 =70 (1.080)5 (0.080) 5/138 7.1 6.9% intergenic (+136/‑264) ymgF/ycgH hypothetical protein/predicted protein; b4491
?REL606 1219720 = 71 (1.190)intergenic (+358/‑42) ymgF/ycgH hypothetical protein/predicted protein; b4491
* ? REL606 = 267891387 (1.340)7 (0.120) 4/132 7.4 8.9% intergenic (+267/‑16) ECB_02509/ECB_02510 Fels‑2 prophage protein/Recombinase
?REL606 = 2679078 67 (1.180)coding (150/1557 nt) ECB_02510 Recombinase
* ? REL606 = 297897125 (1.930)7 (0.120) 4/136 7.5 6.2% coding (117/669 nt) ykgH predicted inner membrane protein
?REL606 = 298061 100 (1.700)intergenic (‑48/+165) ykgH/betA predicted inner membrane protein/choline dehydrogenase
* ? REL606 281778 =71 (1.090)5 (0.090) 4/134 7.5 6.9% intergenic (‑261/‑514) ykgK/ykgL predicted regulator/hypothetical protein
?REL606 281954 = 72 (1.240)intergenic (‑437/‑338) ykgK/ykgL predicted regulator/hypothetical protein
* ? REL606 1519141 =66 (1.020)5 (0.080) 4/138 7.6 6.9% coding (725/957 nt) yddK hypothetical protein
?REL606 1519345 = 74 (1.240)coding (521/957 nt) yddK hypothetical protein
* ? REL606 2042256 =69 (1.060)5 (0.080) 4/140 7.7 6.7% coding (565/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2042511 = 76 (1.260)coding (310/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2041682 =41 (0.630)3 (0.050) 3/128 7.8 6.3% coding (1139/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2041834 = 55 (1.000)coding (987/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 891259102 (1.570)5 (0.080) 4/142 7.8 5.2% coding (282/963 nt) ECB_00831 protein similar to RNA‑directed DNA polymerase from retron EC86
?REL606 = 891492 85 (1.390)coding (515/963 nt) ECB_00831 protein similar to RNA‑directed DNA polymerase from retron EC86
* ? REL606 = 211418260 (0.930)4 (0.060) 4/144 7.9 8.0% coding (492/1968 nt) ECB_02001 hypothetical protein
?REL606 = 2114374 35 (0.560)coding (300/1968 nt) ECB_02001 hypothetical protein