breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1613,7700CA31.6% 265.7 / 43.7 214intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1936,2930T.30.9% 360.5 / 179.3 161intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,2970AG30.7% 320.1 / 172.5 200intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,169,5480GT30.6% 439.4 / 84.7 270P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1936,1690T.29.4% 248.1 / 109.9 106intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH12,169,5770TG28.1% 278.3 / 73.5 262R661R (CGA→CGCrsxCelectron transporter RsxC
*DH12,169,7200CG26.9% 181.9 / 39.8 150E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH12,169,6730AT26.7% 134.6 / 36.4 158R629R (CGT→CGArsxCelectron transporter RsxC
*DH13,184,5850CA26.2% 308.2 / 44.7 149intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH13,906,8370CG26.1% 140.1 / 18.4 92intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH12,326,2870GT25.9% 1001.1 / 97.7 490intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH1613,6130TG25.7% 219.0 / 38.7 219intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,591,5130TG25.4% 246.5 / 49.1 279D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH1936,2781.T25.0% 251.3 / 133.4 104intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,448,5260AC25.0% 768.3 / 85.2 390intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH12,169,5980GT24.8% 304.5 / 34.5 214A654A (GCC→GCArsxCelectron transporter RsxC
*DH12,169,5950GT23.7% 309.1 / 23.3 230V655V (GTC→GTArsxCelectron transporter RsxC
*DH13,447,4030AC23.7% 743.5 / 65.1 365G346G (GGT→GGGmalZalpha‑glycosidase
*DH1613,5970GC22.4% 377.7 / 41.4 268intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,6160GT21.9% 251.9 / 25.8 201V648V (GTC→GTArsxCelectron transporter RsxC

Marginal new junction evidence (lowest skew 10 of 46 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875347 (0.720)179 (0.420) 124/268 6.3 38.9% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 252 (0.590)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =305 (0.700)159 (0.370) 114/268 8.1 34.4% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 1355966 =186 (0.390)137 (0.290)
+CTTC
55/292 27.3 36.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 310 (0.640)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =186 (0.390)106 (0.230)
+CTTC
53/292 28.1 22.7% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 556 (1.150)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =186 (0.390)243 (0.520)
+CTTC
53/292 28.1 57.3% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 = 2590941372 (0.770)77 (0.180) 40/260 30.2 24.9% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 143 (0.340)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 = 1355505400 (0.830)65 (0.160) 37/260 31.6 18.6% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 223 (0.530)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 3183985 =476 (0.990)80 (0.180)
+GATTGTTTCTTC
39/276 32.5 21.1% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 174 (0.360)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 3970042582 (1.210)43 (0.090) 39/282 33.2 7.4% intergenic (‑128/‑86) fimA/ECDH1_RS19620 type‑1 fimbrial protein, A chain/hypothetical protein
?DH1 = 3970338 524 (1.160)intergenic (+22/+58) ECDH1_RS19620/fimE hypothetical protein/tyrosine recombinase
* ? DH1 = 3099328385 (0.800)60 (0.140) 33/260 33.7 17.7% noncoding (54/268 nt) repeat_region repeat region
?DH1 3099693 = 223 (0.530)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys