breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.40.5% 157.3 / 116.3 80intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH13,906,8370CG33.3% 104.2 / 19.6 76intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1936,2930T.32.0% 229.6 / 120.2 99intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1613,7700CA30.1% 246.9 / 21.1 177intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5480GT29.6% 351.1 / 71.8 198P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH12,169,5770TG29.3% 197.7 / 53.8 188R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,448,5260AC28.6% 578.4 / 84.8 345intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1613,5970GC28.3% 281.0 / 38.7 206intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1936,2970AG27.6% 257.0 / 107.3 138intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,184,5850CA26.9% 245.1 / 34.8 135intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH12,326,2870GT26.5% 746.6 / 82.3 376intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH1613,6130TG24.8% 177.4 / 23.6 165intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,591,5130TG24.1% 242.2 / 38.7 241D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH1613,6010GT24.1% 288.1 / 16.9 194intergenic (+462/+561)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH1936,2781.T24.1% 138.4 / 65.7 54intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH14,122,1240CG23.4% 309.9 / 38.8 245intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH13,447,4030AC22.2% 612.5 / 53.1 293G346G (GGT→GGGmalZalpha‑glycosidase
*DH1613,5890GT22.2% 402.8 / 13.8 223intergenic (+450/+573)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5980GT22.0% 254.5 / 10.3 160A654A (GCC→GCArsxCelectron transporter RsxC
*DH12,965,9060TG21.8% 242.5 / 20.2 192intergenic (+139/‑356)aqpZ/ybjEaquaporin/transporter

Marginal new junction evidence (lowest skew 10 of 36 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 1355966 =152 (0.390)116 (0.310)
+CTTC
51/292 24.9 37.0% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 253 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =152 (0.390)90 (0.240)
+CTTC
49/292 25.7 23.8% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 441 (1.130)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =152 (0.390)187 (0.490)
+CTTC
49/292 25.7 55.8% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 1355844 =118 (0.300)78 (0.200)
+C
46/298 27.6 29.7% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 254 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1355505365 (0.940)62 (0.180) 37/260 27.6 20.0% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 181 (0.540)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 3970042448 (1.150)42 (0.110) 38/282 29.6 9.4% intergenic (‑128/‑86) fimA/ECDH1_RS19620 type‑1 fimbrial protein, A chain/hypothetical protein
?DH1 = 3970338 385 (1.050)intergenic (+22/+58) ECDH1_RS19620/fimE hypothetical protein/tyrosine recombinase
* ? DH1 3183985 =360 (0.920)68 (0.190)
+GATTGTTTCTTC
35/276 30.4 22.4% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 151 (0.390)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 1519086248 (0.640)46 (0.120) 35/300 33.0 16.1% coding (71/186 nt) ECDH1_RS07485 hypothetical protein
?DH1 1519099 = 232 (0.600)coding (58/186 nt) ECDH1_RS07485 hypothetical protein
* ? DH1 = 1519281297 (0.760)41 (0.110)
+AACCTTCTAATT
30/276 33.1 13.1% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 297 (0.760)coding (787/900 nt) yfaD transposase
* ? DH1 1355966 =152 (0.390)92 (0.240)
+CTC
30/294 35.0 31.6% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 254 (0.650)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys