breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
RA 143,534 G→A 12.9% L252L (CTG→CTA yadG → putative ABC transporter ATP‑binding protein YadG
RA 256,449 T→C 17.0% intergenic (+14/‑78) gpt → / → frsA xanthine‑guanine phsophoribosyltransferase/fermentation‑respiration switch protein
RA 256,454 C→G 16.2% intergenic (+19/‑73) gpt → / → frsA xanthine‑guanine phsophoribosyltransferase/fermentation‑respiration switch protein
RA 256,459 G→A 18.8% intergenic (+24/‑68) gpt → / → frsA xanthine‑guanine phsophoribosyltransferase/fermentation‑respiration switch protein
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 319,152 C→T 27.4% A284T (GCA→ACA)  rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G 100% L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,750,629 A→G 16.9% Y129H (TAT→CAT)  ydhX ← putative 4Fe‑4S ferredoxin‑type protein
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,368,101 T→C 100% A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,934,179 C→T 23.5% intergenic (‑491/‑56) fucA ← / → fucP L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter
RA 3,041,634 A→C 100% T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,121,334 G→A 20.0% intergenic (‑55/+300) glcA ← / ← glcB glycolate/lactate:H(+) symporter GlcA/malate synthase G
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,991,432 C→T 23.6% P94S (CCA→TCA)  cyaA → adenylate cyclase
JC 4,001,645 Δ5 bp 84.6% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,155,549 C→A 34.0% T183T (ACC→ACA argC → N‑acetylglutamylphosphate reductase
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 27.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366282 366406 125 4 [3] [3] 4 [lacZ] [lacZ]
* * ÷ NC_000913 2066291–2066464 2066577–2066466 3–287 4 [3] [3] 5 [insH‑6] [insH‑6]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 566777 =NA (NA)576 (32.140) 200/262 NT 97.3% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 16 (0.890)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 1207790 =21 (1.160)6 (0.380) 6/232 NT 23.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 20 (1.260)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1560959 =14 (0.780)3 (0.180) 3/242 NT 15.9% coding (1523/1551 nt) ddpA putative D,D‑dipeptide ABC transporter periplasmic binding protein
?NC_000913 1561038 = 19 (1.150)coding (1444/1551 nt) ddpA putative D,D‑dipeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 228473511 (0.610)3 (0.180) 3/248 NT 23.7% coding (360/1761 nt) yejM putative cardiolipin transport protein
?NC_000913 = 2284750 9 (0.530)coding (375/1761 nt) yejM putative cardiolipin transport protein
* ? NC_000913 2662477 =NA (NA)4 (0.230) 4/250 NT NA noncoding (8/37 nt) other REP184d
?NC_000913 2662549 = NA (NA)noncoding (27/37 nt) other REP184f
* ? NC_000913 4472126 =14 (0.780)3 (0.180) 3/246 NT 19.3% coding (270/936 nt) pyrB aspartate carbamoyltransferase catalytic subunit
?NC_000913 4472193 = 12 (0.710)coding (203/936 nt) pyrB aspartate carbamoyltransferase catalytic subunit