breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,672 C→A V488F (GTT→TTT)  mecA ← penicillin binding protein 2 prime
RA 41,171 T→C intergenic (‑38/‑134) mecA ← / → mecR1 penicillin binding protein 2 prime/methicillin resistance regulatory protein MecR1
RA 478,502 C→A D342E (GAC→GAA ndhF → NADH dehydrogenase (ubiquinone)
RA 584,121 G→T intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 597,770 A→T V168V (GTA→GTT tufA → elongation factor EF1A
RA 707,909 A→T M1M (TTG→ATG) † mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 973,213 C→A E175* (GAG→TAG)  yitU ← possible HAD superfamily hydrolase
RA 1,002,200 T→G M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,063,849 C→T D214N (GAC→AAC)  brpA ← LytR family transcriptional regulator
RA 1,105,994 T→C T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,196,508 C→A intergenic (+2563/‑1229) ftsQ → / → ylmE cell division protein FtsQ/hypothetical protein
RA 1,232,148 G→T G18V (GGC→GTC)  prkC → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,539,250 C→G intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,473 C→T A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
JC 1,747,382 (TAC)3→2 coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,027,751 C→G V66L (GTA→CTA)  vraS ← sensor histidine kinase VraS
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,325 C→A Q55H (CAG→CAT ybbP ← hypothetical membrane protein
RA 2,337,959 C→T intergenic (‑125/‑119) adhR ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,358,585 G→C D111E (GAC→GAG rpoA ← DNA‑directed RNA polymerase alpha subunit
RA 2,455,692 Δ1 bp coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =102 (1.110)68 (1.040)
+40 bp
39/200 0.2 48.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA