breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
JC 3,536 (CGGCAGGCG)2→1 14.0% coding (736‑744/933 nt) thrB → homoserine kinase
RA 8,246 C→T 10.2% D3D (GAC→GAT talB → transaldolase B
RA 8,252 G→A 10.9% L5L (TTG→TTA talB → transaldolase B
RA 8,255 C→T 10.5% T6T (ACC→ACT talB → transaldolase B
RA 8,258 C→T 11.1% S7S (TCC→TCT talB → transaldolase B
RA 8,261 T→G 11.1% L8L (CTT→CTG talB → transaldolase B
RA 8,276 C→T 15.2% T13T (ACC→ACT talB → transaldolase B
RA 8,279 A→C 15.9% V14V (GTA→GTC talB → transaldolase B
RA 8,282 G→C 16.4% V15V (GTG→GTC talB → transaldolase B
RA 8,285 C→T 18.3% A16A (GCC→GCT talB → transaldolase B
RA 8,291 T→C 15.1% T18T (ACT→ACC talB → transaldolase B
RA 8,294 G→A 15.1% G19G (GGG→GGA talB → transaldolase B
RA 8,297 C→T 15.3% D20D (GAC→GAT talB → transaldolase B
RA 8,300 C→T 15.7% I21I (ATC→ATT talB → transaldolase B
RA 8,321 A→G 20.2% Q28Q (CAA→CAG talB → transaldolase B
RA 8,324 G→T 19.3% P29P (CCG→CCT talB → transaldolase B
RA 8,336 A→C 25.0% T33T (ACA→ACC talB → transaldolase B
RA 8,339 C→T 26.2% T34T (ACC→ACT talB → transaldolase B
RA 8,348 T→C 26.6% S37S (TCT→TCC talB → transaldolase B
RA 8,351 C→G 26.6% L38L (CTC→CTG talB → transaldolase B
RA 8,357 T→C 27.3% L40L (CTT→CTC talB → transaldolase B
RA 8,358 A→G 27.2% N41D (AAC→GAC) ‡ talB → transaldolase B
RA 8,359 A→G 27.6% N41S (AAC→AGC) ‡ talB → transaldolase B
RA 8,363 A→G 28.7% A42A (GCA→GCG talB → transaldolase B
RA 8,366 G→T 28.7% A43A (GCG→GCT talB → transaldolase B
RA 8,372 T→C 31.8% I45I (ATT→ATC talB → transaldolase B
RA 8,375 G→T 31.6% P46P (CCG→CCT talB → transaldolase B
RA 8,378 A→G 31.7% E47E (GAA→GAG talB → transaldolase B
RA 8,388 T→C 35.3% L51L (TTG→CTG)  talB → transaldolase B
RA 8,396 T→C 36.6% D53D (GAT→GAC talB → transaldolase B
RA 8,399 T→C 37.5% D54D (GAT→GAC talB → transaldolase B
RA 8,414 G→T 32.7% A59A (GCG→GCT talB → transaldolase B
RA 8,415 A→C 33.0% K60Q (AAA→CAA) ‡ talB → transaldolase B
RA 8,416 A→G 33.0% K60R (AAA→AGA) ‡ talB → transaldolase B
RA 8,417 A→T 33.0% K60N (AAA→AAT) ‡ talB → transaldolase B
RA 8,419 A→G 33.1% Q61R (CAG→CGG) ‡ talB → transaldolase B
RA 8,420 G→C 33.6% Q61H (CAG→CAC) ‡ talB → transaldolase B
RA 8,428 A→G 38.4% N64S (AAC→AGC)  talB → transaldolase B
RA 8,448 G→C 41.1% V71L (GTG→CTG) ‡ talB → transaldolase B
RA 8,450 G→C 40.5% V71V (GTG→GTC) ‡ talB → transaldolase B
RA 8,453 C→T 42.4% D72D (GAC→GAT talB → transaldolase B
RA 8,456 G→T 43.8% A73A (GCG→GCT talB → transaldolase B
RA 8,457 A→T 43.1% T74S (ACC→TCC)  talB → transaldolase B
RA 8,462 C→T 43.1% D75D (GAC→GAT talB → transaldolase B
RA 8,465 A→G 43.2% K76K (AAA→AAG talB → transaldolase B
RA 8,471 A→G 44.9% A78A (GCA→GCG talB → transaldolase B
RA 8,474 A→G 42.7% V79V (GTA→GTG talB → transaldolase B
RA 8,477 T→C 42.7% N80N (AAT→AAC talB → transaldolase B
RA 8,480 T→C 42.4% I81I (ATT→ATC talB → transaldolase B
RA 8,486 G→C 46.8% L83L (CTG→CTC talB → transaldolase B
RA 8,492 C→T 50.0% I85I (ATC→ATT talB → transaldolase B
RA 8,502 G→A 53.8% V89I (GTT→ATT) ‡ talB → transaldolase B
RA 8,504 T→C 54.6% V89V (GTT→GTC) ‡ talB → transaldolase B
RA 8,519 A→C 53.4% S94S (TCA→TCC talB → transaldolase B
RA 8,522 T→C 53.0% T95T (ACT→ACC talB → transaldolase B
RA 8,528 T→G 55.4% V97V (GTT→GTG talB → transaldolase B
RA 8,531 T→C 53.0% D98D (GAT→GAC talB → transaldolase B
RA 8,534 G→A 52.4% A99A (GCG→GCA talB → transaldolase B
RA 8,540 T→C 53.0% L101L (CTT→CTC talB → transaldolase B
RA 8,561 A→T 63.2% S108S (TCA→TCT talB → transaldolase B
RA 8,564 T→C 62.1% I109I (ATT→ATC talB → transaldolase B
RA 8,573 A→C 62.3% A112A (GCA→GCC talB → transaldolase B
RA 8,576 A→G 60.8% K113K (AAA→AAG talB → transaldolase B
RA 8,580 C→A 60.3% L115M (CTG→ATG) ‡ talB → transaldolase B
RA 8,582 G→C 60.3% L115L (CTG→CTC) ‡ talB → transaldolase B
RA 8,588 A→G 64.1% K117K (AAA→AAG talB → transaldolase B
RA 8,591 C→G 64.6% L118L (CTC→CTG talB → transaldolase B
RA 8,600 T→C 66.8% D121D (GAT→GAC talB → transaldolase B
RA 8,603 T→G 67.0% A122A (GCT→GCG talB → transaldolase B
RA 8,606 T→C 67.4% G123G (GGT→GGC talB → transaldolase B
RA 8,609 T→C 68.4% I124I (ATT→ATC talB → transaldolase B
RA 8,621 T→C 70.6% R128R (CGT→CGC talB → transaldolase B
RA 8,639 T→C 70.3% A134A (GCT→GCC talB → transaldolase B
RA 8,642 T→C 69.7% S135S (TCT→TCC talB → transaldolase B
RA 8,654 T→C 70.8% G139G (GGT→GGC talB → transaldolase B
RA 8,657 C→T 70.2% I140I (ATC→ATT talB → transaldolase B
RA 8,660 T→C 70.2% R141R (CGT→CGC talB → transaldolase B
RA 8,663 T→C 70.2% A142A (GCT→GCC talB → transaldolase B
RA 8,666 A→T 69.0% A143A (GCA→GCT talB → transaldolase B
RA 8,690 C→T 73.2% I151I (ATC→ATT talB → transaldolase B
RA 8,705 C→G 74.3% T156T (ACC→ACG talB → transaldolase B
RA 8,732 T→C 74.0% R165R (CGT→CGC talB → transaldolase B
RA 8,738 T→C 74.8% C167C (TGT→TGC talB → transaldolase B
RA 8,741 G→C 73.6% A168A (GCG→GCC talB → transaldolase B
RA 8,753 G→C 75.2% V172V (GTG→GTC talB → transaldolase B
RA 8,762 C→T 74.3% I175I (ATC→ATT talB → transaldolase B
RA 8,771 T→C 75.2% F178F (TTT→TTC talB → transaldolase B
RA 8,783 T→C 76.4% I182I (ATT→ATC talB → transaldolase B
RA 8,786 T→C 76.3% L183L (CTT→CTC talB → transaldolase B
RA 8,801 G→C 78.5% A188A (GCG→GCC talB → transaldolase B
RA 8,804 T→C 78.5% N189N (AAT→AAC talB → transaldolase B
RA 8,810 T→C 79.6% D191D (GAT→GAC talB → transaldolase B
RA 8,825 T→A 79.9% A196A (GCT→GCA talB → transaldolase B
RA 8,852 T→C 78.1% S205S (TCT→TCC talB → transaldolase B
RA 8,855 A→C 77.1% V206V (GTA→GTC talB → transaldolase B
RA 8,856 T→A 77.4% S207T (TCT→ACT) ‡ talB → transaldolase B
RA 8,857 C→G 77.4% S207C (TCT→TGT) ‡ talB → transaldolase B
RA 8,858 T→C 78.0% S207S (TCT→TCC) ‡ talB → transaldolase B
RA 8,868 C→G 78.6% Q211E (CAG→GAG)  talB → transaldolase B
RA 8,873 C→T 78.2% Y212Y (TAC→TAT talB → transaldolase B
RA 8,879 A→G 76.1% K214K (AAA→AAG talB → transaldolase B
RA 8,880 G→C 75.4% E215Q (GAG→CAG)  talB → transaldolase B
RA 8,888 T→C 75.6% G217G (GGT→GGC talB → transaldolase B
RA 8,900 G→C 73.7% V221V (GTG→GTC talB → transaldolase B
RA 8,903 T→G 73.8% V222V (GTT→GTG talB → transaldolase B
RA 8,925 A→G 70.8% I230V (ATC→GTC) ‡ talB → transaldolase B
RA 8,927 C→T 70.5% I230I (ATC→ATT) ‡ talB → transaldolase B
RA 8,948 A→T 66.1% A237A (GCA→GCT talB → transaldolase B
RA 8,954 C→T 66.0% C239C (TGC→TGT talB → transaldolase B
RA 8,960 T→C 62.9% R241R (CGT→CGC talB → transaldolase B
RA 8,972 A→C 56.8% A245A (GCA→GCC talB → transaldolase B
RA 8,978 A→G 54.7% A247A (GCA→GCG talB → transaldolase B
RA 8,994 G→T 55.7% A253S (GCG→TCG) ‡ talB → transaldolase B
RA 8,996 G→C 56.1% A253A (GCG→GCC) ‡ talB → transaldolase B
RA 8,999 G→A 56.1% E254E (GAG→GAA talB → transaldolase B
RA 9,008 G→C 57.0% G257G (GGG→GGC talB → transaldolase B
RA 9,011 T→C 57.0% A258A (GCT→GCC talB → transaldolase B
RA 9,012 A→G 57.1% I259V (ATC→GTC) ‡ talB → transaldolase B
RA 9,014 C→T 57.8% I259I (ATC→ATT) ‡ talB → transaldolase B
RA 9,017 A→G 57.4% E260E (GAA→GAG talB → transaldolase B
RA 9,023 A→G 57.2% K262K (AAA→AAG talB → transaldolase B
RA 9,027 T→G 53.4% S264A (TCT→GCT) ‡ talB → transaldolase B
RA 9,028 C→T 52.3% S264F (TCT→TTT) ‡ talB → transaldolase B
RA 9,029 T→A 52.7% S264S (TCT→TCA) ‡ talB → transaldolase B
RA 9,032 C→T 52.7% Y265Y (TAC→TAT talB → transaldolase B
RA 9,038 C→T 55.5% G267G (GGC→GGT talB → transaldolase B
RA 9,058 C→A 57.1% A274E (GCG→GAG)  talB → transaldolase B
RA 9,065 C→T 54.4% I276I (ATC→ATT talB → transaldolase B
RA 9,068 T→C 53.5% T277T (ACT→ACC talB → transaldolase B
RA 9,071 G→A 55.2% E278E (GAG→GAA talB → transaldolase B
RA 9,074 C→T 53.8% S279S (TCC→TCT talB → transaldolase B
RA 9,092 C→T 52.8% H285H (CAC→CAT talB → transaldolase B
RA 9,104 A→G 53.2% P289P (CCA→CCG talB → transaldolase B
RA 9,110 A→G 51.8% A291A (GCA→GCG talB → transaldolase B
RA 9,116 T→C 52.2% D293D (GAT→GAC talB → transaldolase B
RA 9,146 T→G 41.9% A303A (GCT→GCG talB → transaldolase B
RA 9,149 T→C 37.7% I304I (ATT→ATC talB → transaldolase B
RA 9,178 G→A 29.0% G314D (GGC→GAC)  talB → transaldolase B
RA 9,182 T→A 28.3% D315E (GAT→GAA talB → transaldolase B
JC 154,980 Δ18 bp 13.7% coding (617‑634/1398 nt) pcnB ← poly(A) polymerase
RA 225,842 T→C 5.8% T63T (ACT→ACC dkgB → 2,5‑diketo‑D‑gluconate reductase B
RA 360,147 G→C 23.9% intergenic (‑81/+514) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
RA 360,181 G→C 13.0% intergenic (‑115/+480) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
JC 374,639 +GCCC 100% coding (389/1110 nt) frmA ← alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
RA 374,648 Δ1 bp 54.4% coding (380/1110 nt) frmA ← alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
RA 416,070 Δ1 bp 6.0% coding (1024/1320 nt) brnQ → branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
RA 684,503:1 +A 70.5% intergenic (‑34/+296) insH1 ← / ← lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
JC JC 982,570 IS5 (–) +4 bp 52.0% intergenic (‑132/+468) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,005,859 G→A 20.4% G147E (GGG→GAG)  uup → putative transporter subunit of ABC superfamily: ATP‑binding component
RA 1,039,559 G→A 6.7% S36S (AGC→AGT etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,191,676 C→T 8.9% H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T 9.2% T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,230,349 T→C 46.5% intergenic (‑176/‑45) nhaB ← / → fadR sodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator
JC JC 1,254,388 IS5 (+) +4 bp 27.1% coding (142‑145/279 nt) ychH → DUF2583 family putative inner membrane protein
RA 1,484,009 T→C 50.4% pseudogene (213/252 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,072 G→A 49.7% pseudogene (150/252 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,221:1 +T 100% pseudogene (1/252 nt)
pseudogene (750/750 nt)
gapC ←
gapC ←
pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,236 C→G 100% pseudogene (735/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,239 T→C 100% pseudogene (732/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,245 T→C 100% pseudogene (726/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,404 C→T 100% pseudogene (567/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,856 A→T 100% pseudogene (115/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,919 C→G 100% pseudogene (52/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,485,017 A→G 68.5% intergenic (‑47/‑142) gapC ← / → cybB pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
RA 1,566,793 G→A 8.5% D889D (GAC→GAT pqqL ← putative periplasmic M16 family zinc metalloendopeptidase
RA 1,909,758 C→A 20.7% E12* (GAA→TAA)  proQ ← RNA chaperone, putative ProP translation regulator
RA 1,930,220 C→G 24.2% A118P (GCC→CCC)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase
RA 1,977,727 A→C 100% I275R (ATA→AGA)  araG ← fused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components
JC 2,066,645 Δ18 bp 11.5% coding (1626‑1643/3120 nt) flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
MC JC 2,093,044 Δ2,186 bp 100% IS5‑mediated [ugd]wbbL [ugd], gnd, wbbL
RA 2,797,928 C→T 5.6% intergenic (+108/‑246) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine transporter subunit
RA 2,960,118:1 +C 28.3% coding (1676/2247 nt) ptsP ← fused PTS enzyme: PEP‑protein phosphotransferase (enzyme I)/GAF domain containing protein
RA 3,074,446 A→G 5.8% D183D (GAT→GAC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,454 A→C 11.4% F181V (TTC→GTC)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,455 T→G 11.4% A180A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,467 A→G 16.4% G176G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,473 C→T 14.7% K174K (AAG→AAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,478 G→T 14.5% L173M (CTG→ATG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,481 T→A 14.6% T172S (ACG→TCG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,482 A→G 14.5% G171G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,485 C→T 14.8% A170A (GCG→GCA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,490 G→A 14.6% L169L (CTG→TTG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,491 A→T 14.6% S168S (TCT→TCA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,498 A→G 14.5% V166A (GTT→GCT)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,503 G→A 17.7% H164H (CAC→CAT tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,506 G→C 17.9% S163S (TCC→TCG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,521 C→T 20.2% M158I (ATG→ATA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,523 T→A 20.2% M158L (ATG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,538 T→G 24.8% M153L (ATG→CTG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,563 A→G 24.2% I144I (ATT→ATC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,566 G→T 24.3% D143E (GAC→GAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,575 C→G 24.0% P140P (CCG→CCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,578 A→G 23.9% R139R (CGT→CGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,584 A→G 20.6% F137F (TTT→TTC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,587 C→T 20.7% Q136Q (CAG→CAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,590 C→G 18.5% A135A (GCG→GCC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,591 G→C 18.1% A135G (GCG→GGG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,601 T→C 12.9% T132A (ACG→GCG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,602 T→G 13.3% K131N (AAA→AAC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,603 T→C 13.2% K131R (AAA→AGA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,604 T→G 13.2% K131Q (AAA→CAA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,608 T→G 12.9% A129A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,611 A→T 13.0% I128I (ATT→ATA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,617 C→G 13.2% M126I (ATG→ATC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,619 T→A 13.2% M126L (ATG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,623 G→C 13.4% V124V (GTC→GTG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,626 T→G 11.1% A123A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,629 G→A 13.2% N122N (AAC→AAT tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,634 C→T 12.2% A121T (GCC→ACC)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,635 A→G 12.5% I120I (ATT→ATC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,638 A→G 12.5% G119G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,641 C→T 12.4% Q118Q (CAG→CAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,647 C→T 9.5% L116L (CTG→CTA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,649 G→A 9.5% L116L (CTG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,653 A→T 8.4% G114G (GGT→GGA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,659 G→C 7.4% T112T (ACC→ACG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,662 G→C 7.5% T111T (ACC→ACG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,433,446 C→A 100% G315C (GGC→TGC)  rpoA ← RNA polymerase, alpha subunit
JC 3,553,266 Δ8 bp 12.4% coding (693‑700/759 nt) glpR ← GlpR DNA‑binding transcriptional repressor
RA 3,602,774 G→T 6.0% P66P (CCG→CCT yhhQ → DUF165 family inner membrane protein
RA 3,729,296 A→C 100% K320Q (AAG→CAG)  xylR → xylose divergent operon transcriptional activator
RA 3,856,021 T→G 24.8% pseudogene (943/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
JC JC 4,053,617 IS4 (+) +12 bp 30.6% intergenic (+27/‑179) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,076,988 Δ1 bp 100% coding (2195/3051 nt) fdoG ← formate dehydrogenase‑O, large subunit
RA 4,097,480 Δ1 bp 100% coding (1/963 nt) pfkA → 6‑phosphofructokinase I
MC JC 4,097,486 Δ962 bp 100% [pfkA] [pfkA]
JC 4,224,391 Δ12 bp 12.0% coding (706‑717/1650 nt) pgi → glucosephosphate isomerase
MC JC 4,303,167 Δ450 bp 100% coding (1‑450/450 nt) rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,582,841 G→T 7.4% M456I (ATG→ATT tsr → methyl‑accepting chemotaxis protein I, serine sensor receptor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1308888 1308955 68 27 [26] [0] 61 [ompW] [ompW]
* * ÷ CP009273 1857028 1858030 1003 59 [0] [0] 66 [gapA] [gapA]
* * ÷ CP009273 3099986 3100018 33 27 [24] [26] 27 nupG nucleoside transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 15387 =NA (NA)5 (0.090) 5/268 NT 13.9% noncoding (1/1345 nt) IS186 repeat region
?CP009273 = 454251 31 (0.560)intergenic (+95/‑93) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
* ? CP009273 = 287112NA (NA)4 (0.070) 4/276 NT 8.2% noncoding (1/768 nt) IS1 repeat region
?CP009273 2382388 = 45 (0.780)coding (275/423 nt) yfbO uncharacterized protein
* ? CP009273 360104 =52 (0.900)54 (0.940) 49/276 NT 50.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 360180 =58 (1.010)31 (0.980)
+62 bp
27/152 NT 66.1% intergenic (‑114/+481) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 1308956 = 0 (0.000)intergenic (+41/+19) ompW/yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
* ? CP009273 454243 =31 (0.540)7 (0.120) 7/270 NT 18.8% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 454244 =31 (0.540)22 (0.390) 21/268 NT 42.2% intergenic (+88/‑100) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 603467 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? CP009273 = 45425531 (0.540)24 (0.430) 23/268 NT 44.4% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 = 604803NA (NA)35 (0.620) 31/270 NT 48.5% intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
?CP009273 1297568 = 38 (0.660)coding (413/921 nt) oppB oligopeptide transporter subunit
* ? CP009273 1203246 =25 (0.430)10 (0.200) 9/244 NT 28.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 29 (0.570)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326127 (0.470)11 (0.220) 11/244 NT 29.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 29 (0.570)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 129758038 (0.660)22 (0.390) 20/268 NT 37.4% coding (425/921 nt) oppB oligopeptide transporter subunit
?CP009273 2507756 = NA (NA)intergenic (+33/‑54) nupC/insL1 nucleoside (except guanosine) transporter/IS186 transposase
* ? CP009273 1483968 =NA (NA)65 (1.130) 52/276 NT 100% intergenic (+40/+2) aldA/gapC aldehyde dehydrogenase A, NAD‑linked/pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
?CP009273 1858031 = 0 (0.000)intergenic (+8/‑76) gapA/yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
* ? CP009273 = 148508575 (1.300)35 (0.990)
+53 bp
28/170 NT 60.4% intergenic (‑115/‑74) gapC/cybB pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
?CP009273 = 1857027 0 (0.000)intergenic (‑341/‑1) msrB/gapA methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
* ? CP009273 1551001 =49 (0.850)68 (1.190) 59/274 NT 58.5% coding (120/432 nt) osmC lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
?CP009273 = 1558191 48 (0.840)coding (1800/2400 nt) dosP oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
* ? CP009273 3052022 =11 (0.190)949 (16.750)
+AG
193/272 NT 98.9% intergenic (‑253/+3) serA/rpiA D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive
?CP009273 4161679 = NA (NA)noncoding (115/120 nt) rrfB 5S ribosomal RNA of rrnB operon
* ? CP009273 3060603 =49 (0.850)129 (2.240) 97/276 NT 72.5% intergenic (‑71/+96) ygfI/yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase
* ? CP009273 3179455 =NA (NA)63 (1.100) 52/276 NT 100% intergenic (+7/‑91) yqiG/insC1 pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA
?CP009273 = 3426721 0 (0.000)coding (199/1125 nt) smf DNA recombination‑mediator A family protein
* ? CP009273 = 3180785NA (NA)66 (1.150) 56/276 NT 100% pseudogene (6/2445 nt) yqiG pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
?CP009273 3426717 = 0 (0.000)coding (203/1125 nt) smf DNA recombination‑mediator A family protein