breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.33.3% 337.9 / 172.8 138intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1936,2930T.33.3% 336.4 / 188.4 155intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,2970AG32.1% 324.4 / 184.3 204intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH11,065,6180AG31.9% 414.3 / 239.4 314intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH12,169,5480GT30.6% 572.4 / 109.0 350P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1613,7700CA30.4% 346.3 / 33.5 239intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,448,5260AC29.8% 907.2 / 150.0 524intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH13,906,8320CA29.1% 190.3 / 14.1 112intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH12,169,5770TG28.6% 361.9 / 83.5 344R661R (CGA→CGCrsxCelectron transporter RsxC
*DH11,065,6050AT28.6% 451.4 / 174.9 276intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH11,065,6140AG28.5% 450.6 / 212.1 303intergenic (+49/‑14)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH14,122,1240CA27.4% 414.7 / 35.0 338intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly
*DH13,906,8370CG26.7% 190.5 / 23.7 114intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH12,591,5130TG26.1% 303.5 / 57.2 309D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH12,326,2870GT24.7% 1286.2 / 133.0 596intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH12,169,6730AT24.1% 148.3 / 32.9 168R629R (CGT→CGArsxCelectron transporter RsxC
*DH12,591,2610CA23.8% 971.8 / 163.7 483intergenic (+27/+233)ECDH1_RS12810/ECDH1_RS12815tRNA‑Tyr/hypothetical protein
*DH12,169,5680CG23.7% 616.8 / 38.2 340A664A (GCG→GCCrsxCelectron transporter RsxC
*DH13,447,4030AC23.4% 808.1 / 77.4 403G346G (GGT→GGGmalZalpha‑glycosidase
*DH11,165,4440AC23.2% 1046.1 / 77.5 444T71P (ACG→CCG) yfiFmethyltransferase

Marginal new junction evidence (lowest skew 10 of 53 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875500 (0.850)127 (0.240) 93/268 15.8 21.8% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 463 (0.880)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =461 (0.870)81 (0.150) 69/268 23.0 15.0% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 1519086408 (0.690)59 (0.140) 45/220 26.3 17.9% coding (71/186 nt) ECDH1_RS07485 hypothetical protein
?DH1 1519282 = 244 (0.570)coding (787/900 nt) yfaD transposase
* ? DH1 = 2590941517 (0.880)77 (0.150) 49/260 29.7 19.5% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 187 (0.370)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 3183985 =612 (1.040)117 (0.220)
+GATTGTTTCTTC
45/276 33.4 23.8% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 202 (0.340)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 1355505546 (0.930)65 (0.130) 40/260 33.9 14.7% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 283 (0.560)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1519281409 (0.700)68 (0.130)
+AACCTTCTAATT
42/276 34.8 15.3% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 409 (0.700)coding (787/900 nt) yfaD transposase
* ? DH1 1355966 =217 (0.370)107 (0.190)
+CTTC
41/292 37.1 27.1% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 375 (0.640)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =217 (0.370)76 (0.130)
+CTTC
40/292 37.6 15.4% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 639 (1.090)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =217 (0.370)213 (0.370)
+CTTC
40/292 37.6 50.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region