breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 36 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,2930T.31.7% 338.7 / 177.0 151intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1936,1690T.31.1% 179.1 / 84.4 76intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH1613,7700CA30.9% 220.5 / 34.1 212intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,448,5260AC30.2% 618.1 / 106.6 370intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH13,906,8320CA29.2% 154.6 / 10.8 105intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH13,906,8370CG29.1% 162.7 / 26.7 110intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1613,6130TG28.3% 193.9 / 39.5 201intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5480GT28.0% 472.1 / 79.1 246P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH13,184,5850CA28.0% 281.3 / 47.3 143intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM
*DH12,169,6160GT27.4% 227.8 / 26.7 183V648V (GTC→GTArsxCelectron transporter RsxC
*DH12,169,5770TG27.3% 315.9 / 60.7 247R661R (CGA→CGCrsxCelectron transporter RsxC
*DH12,169,7200CG26.8% 157.6 / 48.7 161E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH1936,2781.T26.7% 206.4 / 118.3 86intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,591,2610CA26.2% 719.8 / 144.5 388intergenic (+27/+233)ECDH1_RS12810/ECDH1_RS12815tRNA‑Tyr/hypothetical protein
*DH1936,2970AG26.1% 415.3 / 152.3 207intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,591,5130TG24.8% 256.6 / 40.8 250D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH1613,5970GC24.5% 350.8 / 44.5 247intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,447,4030AC24.2% 684.1 / 90.7 368G346G (GGT→GGGmalZalpha‑glycosidase
*DH12,326,2870GT23.9% 988.2 / 78.9 462intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH14,122,1240CA22.5% 436.9 / 18.8 283intergenic (‑15/+22)ECDH1_RS20430/ECDH1_RS20435tRNA‑Gly/tRNA‑Gly

Marginal new junction evidence (lowest skew 10 of 53 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 1355966 =174 (0.370)125 (0.280)
+CTTC
58/292 20.9 36.4% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 275 (0.590)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =174 (0.370)94 (0.210)
+CTTC
54/292 22.2 21.8% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 521 (1.120)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =174 (0.370)216 (0.480)
+CTTC
54/292 22.2 56.1% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 = 2590941412 (0.890)66 (0.160) 33/260 27.5 21.2% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 134 (0.330)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 = 1519281278 (0.600)47 (0.110)
+AACCTTCTAATT
33/276 29.0 15.5% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 278 (0.600)coding (787/900 nt) yfaD transposase
* ? DH1 3183985 =476 (1.020)71 (0.170)
+GATTGTTTCTTC
32/276 29.4 19.3% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 168 (0.360)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 1355505434 (0.930)58 (0.140) 27/260 30.4 16.0% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 234 (0.580)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355847 =111 (0.240)46 (0.100)
+CCTC
25/292 34.3 19.6% noncoding (75/76 nt) ECDH1_RS06680 tRNA‑Val
?DH1 = 3099750 276 (0.590)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 2326110 =NA (NA)28 (0.070)
+22 bp
19/256 34.6 NA intergenic (+30/+377) yddM/fdnI transcriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
?DH1 = 2326443 NA (NA)noncoding (155/176 nt) repeat_region repeat region
* ? DH1 1355843 =109 (0.230)126 (0.270)
+C
24/298 35.3 39.8% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 275 (0.590)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys