breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 164,581 A→C 19.5% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 357,775 A→T 6.0% intergenic (+321/+16) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 357,778 C→T 6.2% intergenic (+324/+13) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 475,971 C→G 5.6% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 5.6% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 580,759 G→C 5.6% intergenic (‑314/‑75) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
RA 777,208 A→C 11.3% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 781,238 T→A 8.4% intergenic (+16/‑131) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,254 A→T 6.7% intergenic (+32/‑115) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,269 C→T 8.4% intergenic (+47/‑100) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 882,356 A→T 6.1% F127I (TTT→ATT)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
RA 1,466,201 T→C 6.4% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,210 G→A 7.2% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,336 C→G 9.0% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,345 T→C 6.5% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,708,709 T→A 7.1% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,805 T→A 9.0% R629R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,814 A→T 6.9% A632A (GCA→GCT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
JC JC 1,755,531 IS2 (–) +5 bp 22.4% intergenic (‑390/‑163) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I
RA 1,756,152 T→C 5.1% L152P (CTG→CCG)  pykF → pyruvate kinase I
RA 1,756,507 Δ1 bp 14.7% coding (810/1413 nt) pykF → pyruvate kinase I
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,140,484 G→A 5.6% Q377* (CAG→TAG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,228 C→A 11.7% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,234 C→A 19.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,245 G→T 18.2% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,275 C→A 6.2% intergenic (‑71/+237) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,177,279 C→A 6.8% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,229,177 A→C 14.3% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C
RA 2,286,311 C→G 5.4% intergenic (+24/+79) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,313 T→A 5.8% intergenic (+26/+77) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,315 C→G 5.4% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,304,649 C→T 6.1% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,688 A→G 8.4% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,575,877 A→C 6.4% intergenic (‑72/+221) eutS ← / ← maeB putative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/phosphotransacetylase
RA 2,637,433 C→T 5.4% intergenic (‑77/+41) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
RA 2,637,438 T→G 5.3% intergenic (‑82/+36) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
RA 2,637,439 C→A 5.3% intergenic (‑83/+35) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
RA 2,637,444 A→G 5.3% intergenic (‑88/+30) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
RA 2,653,367 G→A 6.9% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,653,372 T→C 6.7% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 3,046,050 T→G 7.0% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,239,609 T→G 7.9% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,368,173 C→T 5.3% P116L (CCG→CTG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,180 A→G 5.1% T118T (ACA→ACG yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,181 C→G 5.5% P119A (CCG→GCG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,368,182 C→T 5.4% P119L (CCG→CTG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,504,880 A→C 5.4% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,728,109 A→C 7.2% D421E (GAT→GAG xylB ← xylulokinase
RA 3,728,120 T→G 5.3% T418P (ACG→CCG)  xylB ← xylulokinase
RA 3,728,125 T→G 5.4% Y416S (TAC→TCC)  xylB ← xylulokinase
RA 3,781,022 T→G 10.6% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,038 T→A 15.3% intergenic (+21/‑177) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,051 C→G 5.8% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,067 T→G 10.5% intergenic (+50/‑148) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,131 T→G 6.6% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,132 C→G 5.3% intergenic (+115/‑83) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,138 A→C 6.9% intergenic (+121/‑77) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,158 A→C 5.7% intergenic (+141/‑57) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,828,980 A→C 6.8% Q12H (CAA→CAC xanP → xanthine permease
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,908,506 C→G 5.7% intergenic (‑140/+43) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease
RA 3,959,482 A→C 8.1% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,015,085 A→T 7.8% E678V (GAA→GTA)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
RA 4,070,462 T→A 5.2% intergenic (‑61/+53) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,070,463 T→A 5.3% intergenic (‑62/+52) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,070,464 T→A 5.5% intergenic (‑63/+51) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,189,448 C→T 24.6% Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,060 C→T 28.7% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,155 T→C 8.5% intergenic (+361/+281) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,362,578 G→T 72.0% noncoding (49/76 nt) pheU ← tRNA‑Phe
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,412,794 A→T 6.3% L136F (TTA→TTT yjfM → DUF1190 family protein
RA 4,520,500 Δ1 bp 6.2% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 6.2% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,633,657 C→T 5.1% C30Y (TGC→TAC)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772991 774224 1234 169 [163] [168] 172 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037683 1039331 1649 171 [161] [167] 169 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 1166010 1167444 1435 170 [165] [168] 169 ndh ndh
* * ÷ NC_000913 3423722–3424560 3424560 1–839 180 [168] [162] 172 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)6 (0.020) 5/264 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 = 213635NA (NA)5 (0.020) 4/264 NT NA noncoding (4/37 nt) REP15 (repetitive extragenic palindromic) element; contains 1 REP sequences REP15 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 213668 NA (NA)noncoding (37/37 nt) REP15 (repetitive extragenic palindromic) element; contains 1 REP sequences REP15 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 274620 =NA (NA)7 (0.020) 4/260 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 274695NA (NA)6 (0.020) 3/270 NT NA noncoding (455/1195 nt) IS5 repeat region
?NC_000913 = 274727 NA (NA)noncoding (423/1195 nt) IS5 repeat region
* ? NC_000913 = 315642NA (NA)6 (0.020) 4/274 NT NA noncoding (414/1255 nt) IS3 repeat region
?NC_000913 = 315712 NA (NA)noncoding (484/1255 nt) IS3 repeat region
* ? NC_000913 = 381689NA (NA)3 (0.010) 3/260 NT NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 = 445266NA (NA)3 (0.010) 3/268 NT NA noncoding (67/87 nt) REP40 (repetitive extragenic palindromic) element; contains 2 REP sequences REP40 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 = 445281 NA (NA)noncoding (82/87 nt) REP40 (repetitive extragenic palindromic) element; contains 2 REP sequences REP40 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 781577 =237 (0.690)100 (0.300)
+GAAG
19/270 NT 32.7% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 187 (0.540)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 1180354 =NA (NA)7 (0.020) 3/264 NT NA noncoding (1/96 nt) RIP104 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP104 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 1180379 = NA (NA)noncoding (26/96 nt) RIP104 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP104 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 1207790 =115 (0.340)115 (0.380) 68/246 NT 50.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 127 (0.420)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805114 (0.330)157 (0.520) 91/246 NT 58.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 127 (0.420)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1287084135 (0.390)10 (0.030) 6/238 NT 6.8% noncoding (153/171 nt) rttR rtT sRNA, processed from tyrT transcript
?NC_000913 1287557 = 160 (0.550)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 12994980 (0.000)234 (0.700) 113/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1954508NA (NA)6 (0.020) 6/264 NT NA noncoding (80/98 nt) RIP139 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP139 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 1954519 NA (NA)noncoding (91/98 nt) RIP139 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP139 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 2700550 =269 (0.780)16 (0.050) 12/240 NT 7.1% noncoding (31/77 nt) ohsC sRNA antisense regulator of shoB toxin
?NC_000913 2700601 = 189 (0.640)intergenic (+5/+17) ohsC/acpS sRNA antisense regulator of shoB toxin/holo‑[acyl‑carrier‑protein] synthase 1
* ? NC_000913 2727891 =NA (NA)10 (0.030) 5/266 NT NA noncoding (1294/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 2727910 = NA (NA)noncoding (1275/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 = 2817942235 (0.680)12 (0.040) 11/238 NT 5.2% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 233 (0.790)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 3304504NA (NA)5 (0.020) 5/266 NT NA noncoding (1/29 nt) REP241 (repetitive extragenic palindromic) element; contains 1 REP sequences REP241 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 3304536 NA (NA)intergenic (+81/+37) yhbW/mtr putative luciferase‑like monooxygenase/tryptophan transporter of high affinity
* ? NC_000913 3740964 =NA (NA)12 (0.040) 7/264 NT NA noncoding (1/98 nt) RIP269 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP269 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3740989 = NA (NA)noncoding (26/98 nt) RIP269 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP269 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 3774236 =NA (NA)10 (0.030) 4/264 NT NA noncoding (1/99 nt) RIP271 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP271 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3774261 = NA (NA)noncoding (26/99 nt) RIP271 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP271 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 4037305 =NA (NA)3 (0.010) 3/270 NT 60.7% noncoding (46/76 nt) alaT tRNA‑Ala
?NC_000913 4037328 = 2 (0.010)noncoding (69/76 nt) alaT tRNA‑Ala
* ? NC_000913 = 4040683261 (0.760)40 (0.120) 9/274 NT 11.8% intergenic (+47/+223) rrfA/mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
?NC_000913 = 4213162 338 (1.000)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein