breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,085 T→A 100% I690N (ATC→AAC)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 272,330:1 +G 15.4% intergenic (+140/‑250) insN → / → eyeA CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA
RA 463,457 G→A 45.0% G515S (GGT→AGT)  ppiD → periplasmic folding chaperone
JC JC 568,268 IS1 (–) +9 bp 100% intergenic (+21/‑39) renD → / → emrE DLP12 prophage; protein RenD/multidrug/betaine/choline efflux transporter EmrE
RA 699,559 A→G 26.1% intergenic (‑382/+15) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 1,196,220 C→T 100% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 100% T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C 100% L375L (TTA→CTA)  icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,570,512 C→T 100% intergenic (‑23/+133) gadC ← / ← gadB L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,323,330 C→T 29.5% intergenic (+79/‑117) atoC → / → atoD DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC/acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
RA 2,534,156 A→T 40.0% I31F (ATT→TTT)  ptsI → PTS enzyme I
RA 2,661,816 T→G 71.0% T106P (ACC→CCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,398 T→G 100% L29R (CTT→CGT)  ygfZ → folate‑binding protein
RA 3,351,007 Δ1 bp 35.3% coding (2019/2337 nt) arcB ← sensory histidine kinase ArcB
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,137,914 C→T 8.3% intergenic (‑257/+18) yijF ← / ← gldA conserved protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 30.5% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,324,917 T→C 13.2% K83K (AAA→AAG phnC ← phosphonate ABC transporter ATP binding subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196297 1211435 15139 4 [3] [3] 4 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2066293–2066464 2066611–2066466 3–319 4 [3] [3] 4 [insH‑6] [insH‑6]
* * ÷ NC_000913 3423754–3424234 3424514–3424240 7–761 4 [3] [3] 4 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3621199–3622296 3653127–3652031 29736–31929 4 [3] [2] 4 [rhsB]–[insH‑11] [rhsB],yhhH,yhhI,yhhJ,rbbA,yhiI,yhiJ,yhiL,yhiM,yhiN,pitA,uspB,uspA,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,[insH‑11]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 285319 =10 (0.480)3 (0.160) 3/244 NT 18.6% intergenic (+151/‑76) yagF/yagG CP4‑6 prophage; D‑xylonate dehydratase/putative D‑xylonate transporter YagG
?NC_000913 285411 = 17 (0.890)coding (17/1383 nt) yagG putative D‑xylonate transporter YagG
* ? NC_000913 = 609351NA (NA)6 (0.300) 6/256 NT 35.3% noncoding (1345/1345 nt) IS186 repeat region
?NC_000913 2227427 = 11 (0.550)pseudogene (1332/1436 nt) mdtQ putative multidrug resistance outer membrane protein MdtQ
* ? NC_000913 = 2067353NA (NA)5 (0.250) 4/258 NT 29.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2079471 = 12 (0.580)intergenic (‑110/+62) yeeX/yeeA DUF496 domain‑containing protein YeeX/putative transporter YeeA
* ? NC_000913 = 33520234 (0.190)6 (0.290) 6/262 NT 60.0% coding (1003/2337 nt) arcB sensory histidine kinase ArcB
?NC_000913 3365556 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 4012917 =30 (1.450)4 (0.200) 4/256 NT 12.3% intergenic (‑101/‑136) metR/metE DNA‑binding transcriptional dual regulator MetR/cobalamin‑independent homocysteine transmethylase
?NC_000913 4013018 = 28 (1.400)intergenic (‑202/‑35) metR/metE DNA‑binding transcriptional dual regulator MetR/cobalamin‑independent homocysteine transmethylase
* ? NC_000913 4306577 =30 (1.450)3 (0.150) 3/250 NT 9.9% coding (21/1986 nt) yjcS alkyl sulfatase
?NC_000913 4306596 = 26 (1.330)coding (2/1986 nt) yjcS alkyl sulfatase