breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 754,446 A→G 100% L8P (CTC→CCC)  gltA ← citrate synthase
RA 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 940,751 T→C 10.0% intergenic (+31/‑208) serS → / → dmsA serine‑‑tRNA ligase/dimethyl sulfoxide reductase subunit A
RA 940,754 G→A 10.5% intergenic (+34/‑205) serS → / → dmsA serine‑‑tRNA ligase/dimethyl sulfoxide reductase subunit A
RA 1,074,945 T→G 18.1% N1313T (AAC→ACC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,285,190 T→A 13.4% L18I (TTA→ATA)  narJ → nitrate reductase 1 molybdenum cofactor assembly chaperone
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,572,964 C→T 12.1% A747T (GCA→ACA)  pqqL ← putative zinc peptidase
RA 1,666,468 A→T 14.2% Q385L (CAG→CTG)  clcB → putative chloride:H(+) antiporter ClcB
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 1,985,112 C→G 17.4% intergenic (‑43/+27) araG ← / ← araF arabinose ABC transporter ATP binding subunit/arabinose ABC transporter periplasmic binding protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,368,101 T→C 100% A21A (GCT→GCC arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,716,030 T→G 16.0% intergenic (+20/+36) eamB → / ← grcA cysteine/O‑acetylserine exporter EamB/stress‑induced alternate pyruvate formate‑lyase subunit
RA 3,041,634 A→C 100% T108P (ACC→CCC)  ygfZ → folate‑binding protein
RA 3,072,800 C→T 11.1% D298N (GAT→AAT)  epd ← D‑erythrose‑4‑phosphate dehydrogenase
RA 3,072,802 A→T 11.5% V297D (GTC→GAC)  epd ← D‑erythrose‑4‑phosphate dehydrogenase
RA 3,441,060 T→C 14.9% K206E (AAG→GAG)  rpsD ← 30S ribosomal subunit protein S4
RA 3,441,061 G→C 14.4% S205S (TCC→TCG) ‡ rpsD ← 30S ribosomal subunit protein S4
RA 3,441,062 G→A 14.6% S205F (TCC→TTC) ‡ rpsD ← 30S ribosomal subunit protein S4
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,733,502 G→A 12.7% R434R (AGG→AGA xylG → xylose ABC transporter ATP binding subunit
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,094,581 A→G 13.6% intergenic (‑309/+142) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,236,011 T→C 18.2% S36P (TCG→CCG)  yjbE → uncharacterized protein YjbE
RA 4,296,060 C→T 37.0% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,365,452 G→T 12.0% intergenic (‑96/+20) cutA ← / ← dcuA copper binding protein CutA/C4‑dicarboxylate transporter DcuA
RA 4,365,453 A→C 12.1% intergenic (‑97/+19) cutA ← / ← dcuA copper binding protein CutA/C4‑dicarboxylate transporter DcuA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2066278–2066604 2066604 1–327 9 [8] [7] 9 [insH‑6] [insH‑6]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315646NA (NA)4 (0.150) 4/254 NT NA noncoding (418/1255 nt) IS3 repeat region
?NC_000913 = 315665 NA (NA)noncoding (437/1255 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1027 (37.840) 232/262 NT 97.4% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 28 (1.020)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 120223526 (0.950)3 (0.120) 3/250 NT 10.6% intergenic (‑151/+24) ymfJ/ymfK e14 prophage; protein YmfJ/e14 prophage; putative repressor protein YmfK
?NC_000913 = 1202239 26 (1.000)intergenic (‑155/+20) ymfJ/ymfK e14 prophage; protein YmfJ/e14 prophage; putative repressor protein YmfK
* ? NC_000913 1207790 =20 (0.730)7 (0.290) 7/232 NT 29.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 16 (0.670)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780521 (0.770)4 (0.170) 4/232 NT 18.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 16 (0.670)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 2252869NA (NA)4 (0.150) 3/250 NT NA noncoding (19/28 nt) other REPv157b
?NC_000913 = 2252879 NA (NA)noncoding (9/28 nt) other REPv157b
* ? NC_000913 2727891 =NA (NA)4 (0.150) 4/252 NT NA noncoding (1294/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 2727910 = NA (NA)noncoding (1275/2904 nt) rrlG 23S ribosomal RNA