breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 614,751 G→T E199* (GAA→TAA)  entF → apo‑serine activating enzyme
RA 622,465 C→A L56I (CTT→ATT)  entS → enterobactin exporter EntS
RA 730,644 A→G R354R (CGA→CGG rhsC → rhs element protein RhsC
RA 766,899 C→T L306L (CTG→TTG)  mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 885,020 G→T P71T (CCT→ACT)  ybjH ← uncharacterized protein YbjH
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,020,937 T→C intergenic (‑18/‑201) sulA ← / → sxy cell division inhibitor SulA/transcriptional coactivator for CRP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,242,292 Δ5 bp coding (785‑789/915 nt) ldcA ← murein L,D‑carboxypeptidase
RA 1,269,117 C→G intergenic (+98/+51) kdsA → / ← ldrA 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase/small toxic polypeptide LdrA
RA 1,351,376 G→T intergenic (‑337/+31) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,703,301 G→T S336S (TCC→TCA ydgJ ← putative oxidoreductase YdgJ
RA 1,727,199 C→T L181F (CTC→TTC)  nemA → N‑ethylmaleimide reductase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,053,832 C→A L64I (CTC→ATC)  shiA → shikimate:H(+) symporter
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,630,356 G→T A11S (GCC→TCC)  yfgI → nalidixic acid resistance protein YfgI
RA 2,998,998 G→T noncoding (60/74 nt) glyU ← tRNA‑Gly
RA 3,110,533 G→A intergenic (+92/+57) pheV → / ← yghD tRNA‑Phe/putative type II secretion system M‑type protein
RA 3,309,282 C→A V629V (GTG→GTT pnp ← polynucleotide phosphorylase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,453,657 C→A E91* (GAA→TAA)  gspB ← putative general secretion pathway protein B
JC JC 3,455,388 IS1 (+) +9 bp coding (3‑11/1470 nt) gspA ← Type II secretion system protein GspA
JC JC 3,465,602 IS1 (–) +9 bp coding (60‑68/678 nt) gspO → Type II secretion system prepilin peptidase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,604,784 G→T R131L (CGC→CTC)  rsmD → 16S rRNA m(2)G966 methyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,926,265 T→C D64G (GAC→GGC)  mioC ← flavoprotein MioC
RA 3,941,541 G→T intergenic (‑214/‑267) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 3,942,012 A→G noncoding (205/1542 nt) rrsC → 16S ribosomal RNA
RA 4,149,878 C→A A130S (GCC→TCC)  eptC ← phosphoethanolamine transferase EptC
RA 4,189,902 G→T E39D (GAG→GAT yjaZ → conserved protein YjaZ
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,573,173 IS5 (–) +4 bp coding (760‑763/1518 nt) yjiT → putative uncharacterized protein YjiT

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211433 15157 17 [13] [10] 17 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3103000 3104084 1085 17 [16] [13] 17 mutY mutY
* * ÷ NC_000913 3423768–3424234 3424525–3424242 9–758 17 [16] [15] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 608010 =NA (NA)56 (0.840) 40/274 0.5 94.9% noncoding (4/1345 nt) IS186 repeat region
?NC_000913 = 1503204 3 (0.050)noncoding (44/1748 nt) IS609 repeat region
* ? NC_000913 = 609351NA (NA)67 (1.030) 43/268 0.3 NA noncoding (1345/1345 nt) IS186 repeat region
?NC_000913 1503198 = NA (NA)noncoding (38/1748 nt) IS609 repeat region
* ? NC_000913 = 12994980 (0.000)80 (1.230) 56/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2904195 =22 (0.330)29 (0.550) 22/216 1.1 65.0% intergenic (+821/+552) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
?NC_000913 = 2904255 14 (0.270)intergenic (+881/+492) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
* ? NC_000913 3363001 =91 (1.360)113 (1.700) 58/274 0.1 71.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)73 (1.090) 46/276 0.3 96.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA