breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 274,977 T→C I204I (ATT→ATC Cgl0255 → Catalase
MC JC 335,027 1067 bp→22 bp coding (19‑1085/1107 nt) Cgl0319 → Zn‑dependent alcohol dehydrogenases, class III
RA 364,912 A→C E363D (GAA→GAC Cgl0344 → ATPases involved in chromosome partitioning
RA 387,460 T→C *66* (TAA→TAG Cgl0365 ← Hypothetical protein
RA 671,707 Δ1 bp coding (8/957 nt) Cgl0657 → Uncharacterized protein involved in propionate catabolism
RA 736,480 T→C V23A (GTG→GCG)  Cgl0716 → Hypothetical protein
RA 770,809 G→A L1112F (CTT→TTT)  Cgl0738 ← Type II restriction enzyme, methylase subunits
RA 1,142,255 T→C L328S (TTG→TCG)  Cgl1095 → Hypothetical membrane protein
RA 1,279,070 T→G D461E (GAT→GAG Cgl1212 → F0F1‑type ATP synthase beta subunit
RA 1,457,949 C→T T301T (ACC→ACT Cgl1389 → Phenylalanyl‑tRNA synthetase alpha subunit
RA 1,699,535 C→T S288N (AGC→AAC)  Cgl1603 ← S‑adenosylmethionine synthetase
RA 1,751,737 G→A V81I (GTC→ATC)  Cgl1651 → Hypothetical protein
RA 1,810,032 G→A R91H (CGC→CAC)  Cgl1713 → Site‑specific recombinases, DNA invertase Pin homologs
RA 2,003,379 C→T intergenic (+42/+20) Cgl1901 → / ← Cgl1902 Ribonuclease D/Deoxyxylulose‑5‑phosphate synthase
RA 2,036,724 A→C intergenic (‑315/‑88) Cgl1929 ← / → Cgl1930 Hypothetical protein/SOS‑response transcriptional repressors (RecA‑mediated autopeptidases)
RA 2,041,951 G→T G75V (GGT→GTT)  Cgl1934 → Transcriptional regulators of sugar metabolism
RA 2,058,943 A→G S160P (TCT→CCT)  Cgl1949 ← 2‑methylthioadenine synthetase
RA 2,060,507 C→T intergenic (+5/‑113) Cgl1950 → / → Cgl1951 ABC‑type transporter, ATPase component (glutamate)/ABC‑type transporter, periplasmic component (glutamate)
RA 2,060,571 C→A intergenic (+69/‑49) Cgl1950 → / → Cgl1951 ABC‑type transporter, ATPase component (glutamate)/ABC‑type transporter, periplasmic component (glutamate)
RA 2,083,859 T→A G444G (GGA→GGT Cgl1975 ← Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)
RA 2,126,262 G→C D67H (GAC→CAC)  Cgl2012 → ABC‑type transporter, ATPase component
RA 2,160,737 C→T P23L (CCT→CTT)  Cgl2047 → Hypothetical protein
RA 2,425,773 G→A F61F (TTC→TTT Cgl2292 ← Coproporphyrinogen III oxidase and related FeS oxidoreductases
RA 2,979,996 C→T H338H (CAC→CAT Cgl2795 → Hypothetical protein
MC JC 2,980,216 1466 bp→22 bp coding (19‑1484/1521 nt) Cgl2796 ← NAD‑dependent aldehyde dehydrogenases
RA 2,981,696 T→A T2S (ACT→TCT)  Cgl2796 ← NAD‑dependent aldehyde dehydrogenases
JC 2,990,100 Δ15 bp coding (3174‑3188/3336 nt) Cgl2804 ← Hypothetical protein
RA 3,035,143 G→A R321K (AGG→AAG)  Cgl2846 → Hypothetical membrane protein
RA 3,126,458 T→C A22A (GCT→GCC Cgl2927 → Superoxide dismutase
RA 3,155,328 T→C Q457Q (CAA→CAG Cgl2951 ← Predicted transposase
RA 3,156,189 T→G V170V (GTA→GTC Cgl2951 ← Predicted transposase
RA 3,189,874 C→T intergenic (‑221/‑4) Cgl2987 ← / → Cgl2988 Hypothetical protein/Transcriptional regulators
RA 3,208,522 +T coding (243/1179 nt) Cgl3007 → Malic enzyme
RA 3,286,754 G→A E44E (GAG→GAA Cgl3077 → Hypothetical membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ BA000036 1693265 1693900 636 103 [0] [0] 75 Cgl1598 Pentose‑5‑phosphate‑3‑epimerase
* * ÷ BA000036 1961663–1962273 1962935 663–1273 48 [46] [46] 48 Cgl1848 Hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? BA000036 1114357 =NA (NA)76 (0.820) 68/594 0.5 95.0% intergenic (+35/‑129) Cgl1065/Cgl1066 Permeases of the major facilitator superfamily/Transposase
?BA000036 = 1413173 4 (0.040)intergenic (‑257/+392) Cgl1352/codon recognized:TTG Hypothetical membrane protein/tRNA‑Leu
* ? BA000036 1114358 =NA (NA)100 (1.090) 84/592 0.1 100% intergenic (+36/‑128) Cgl1065/Cgl1066 Permeases of the major facilitator superfamily/Transposase
?BA000036 = 1693264 0 (0.000)coding (655/660 nt) Cgl1598 Pentose‑5‑phosphate‑3‑epimerase
* ? BA000036 = 1115809NA (NA)102 (1.110) 92/594 0.0 98.1% intergenic (+13/+20) Cgl1066/Cgl1067 Transposase/Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes
?BA000036 1413166 = 2 (0.020)intergenic (‑250/+399) Cgl1352/codon recognized:TTG Hypothetical membrane protein/tRNA‑Leu
* ? BA000036 = 1115810NA (NA)56 (0.720)
+46 bp
50/502 0.9 100% intergenic (+14/+19) Cgl1066/Cgl1067 Transposase/Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes
?BA000036 1693901 = 0 (0.000)coding (18/660 nt) Cgl1598 Pentose‑5‑phosphate‑3‑epimerase
* ? BA000036 1825711 =103 (1.120)77 (0.840) 66/594 0.5 59.7% intergenic (‑105/+37) Cgl1731/Cgl1733 Hypothetical protein/Putative transposase
?BA000036 1828006 = 1 (0.010)intergenic (‑1069/+1679) Cgl1734/Cgl1735 Transposase/Hypothetical protein
* ? BA000036 = 182700379 (0.860)91 (0.990) 72/594 0.3 68.9% intergenic (‑66/+2682) Cgl1734/Cgl1735 Transposase/Hypothetical protein
?BA000036 = 1828008 3 (0.030)intergenic (‑1071/+1677) Cgl1734/Cgl1735 Transposase/Hypothetical protein