mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 49,252 | G→C | 21.2% | Q208H (CAG→CAC) | yabE → | putative cell wall shaping enzyme |
| RA | BS168_reference_genome | 96,240 | G→A | 7.9% | intergenic (+19/‑152) | trnJ‑Ala → / → rrnW‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| RA | BS168_reference_genome | 143,659 | C→T | 100% | S100F (TCT→TTT) | rpsE → | ribosomal protein S5 |
| RA | BS168_reference_genome | 154,264 | C→T | 5.0% | Y141Y (TAC→TAT) | rplM → | ribosomal protein L13 |
| RA | BS168_reference_genome | 165,749 | Δ1 bp | 100% | intergenic (+42/‑5) | rrnI‑5S → / → trnI‑Asn | ribosomal RNA‑5S/tRNA‑Asn |
| RA | BS168_reference_genome | 165,751 | Δ1 bp | 100% | intergenic (+44/‑3) | rrnI‑5S → / → trnI‑Asn | ribosomal RNA‑5S/tRNA‑Asn |
| RA | BS168_reference_genome | 165,825:1 | +C | 100% | noncoding (72/72 nt) | trnI‑Asn → | tRNA‑Asn |
| RA | BS168_reference_genome | 166,037:1 | +T | 100% | intergenic (+4/‑27) | trnI‑Gly → / → trnI‑Arg | tRNA‑Gly/tRNA‑Arg |
| RA | BS168_reference_genome | 166,344 | Δ1 bp | 100% | intergenic (+16/‑156) | trnI‑Ala → / → rrnH‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| RA | BS168_reference_genome | 353,447 | T→G | 10.3% | V197G (GTG→GGG) | ffoR → | Fur‑regulated NADPH:ferredoxin oxidoreductase |
| RA | BS168_reference_genome | 376,018 | Δ1 bp | 100% | intergenic (‑220/‑14) | hxlA ← / → hxlR | 3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing) |
| RA | BS168_reference_genome | 377,502 | C→T | 5.7% | H179Y (CAT→TAT) | srfAA → | surfactin synthetase |
| RA | BS168_reference_genome | 389,065 | T→G | 14.5% | L441R (CTT→CGT) | srfAB → | surfactin synthetase |
| RA | BS168_reference_genome | 409,328 | A→G | 7.7% | D208D (GAT→GAC) | tcyC ← | cystine ABC transporter (ATP‑binding protein) |
| RA | BS168_reference_genome | 432,699 | T→G | 20.1% | F110V (TTT→GTT) | pbtN → | petrobactin iron‑siderophore ABC transporter (permease) |
| RA | BS168_reference_genome | 457,443 | A→T | 5.4% | I141F (ATT→TTT) | pxpA → | oxoprolinase subunit A |
| RA | BS168_reference_genome | 463,401 | G→T | 17.6% | E53* (GAA→TAA) | yczI → | conserved protein of unknown function |
| RA | BS168_reference_genome | 507,401 | T→G | 14.7% | V179G (GTT→GGT) | ydbO → | putative cation efflux system |
| RA | BS168_reference_genome | 515,020 | T→A | 28.9% | Y199F (TAT→TTT) | ydcA ← | putative rhomboid protease |
| RA | BS168_reference_genome | 515,043 | G→C | 28.4% | C191W (TGC→TGG) | ydcA ← | putative rhomboid protease |
| RA | BS168_reference_genome | 515,053 | G→C | 54.8% | S188W (TCG→TGG) | ydcA ← | putative rhomboid protease |
| RA | BS168_reference_genome | 538,088 | G→A | 6.5% | G10S (GGC→AGC) | conC → | ICEBs1 mobile element: subunit of the conjugation machinery |
| RA | BS168_reference_genome | 557,865:1 | +T | 100% | intergenic (+416/‑8) | yddT → / → ydzN | putative exported phage protein/hypothetical protein |
| RA | BS168_reference_genome | 608,185 | G→T | 20.7% | intergenic (‑110/+61) | ydgF ← / ← dinB | putative amino acid permease/nuclease inhibitor |
| RA | BS168_reference_genome | 648,025 | T→G | 14.5% | F29C (TTT→TGT) | tatCY → | component of the twin‑arginine pre‑protein translocation pathway |
| RA | BS168_reference_genome | 670,425 | T→G | 11.5% | A113A (GCT→GCG) | ydjE → | putative sugar kinase (ribokinase family); prophage region 3 |
| RA | BS168_reference_genome | 672,996 | T→A | 5.2% | L335M (TTG→ATG) | ydjG → | putative phage replication protein; prophage region 3 |
| RA | BS168_reference_genome | 754,709 | C→T | 5.2% | T77I (ACA→ATA) | yesE → | conserved hypothetical protein |
| RA | BS168_reference_genome | 892,022 | A→T | 100% | N196I (AAT→ATT) | malR → | transcriptional activator of the Mal operon |
| RA | BS168_reference_genome | 944,551 | C→T | 100% | P22L (CCT→CTT) | perR → | transcriptional regulator (Fur family) |
| RA | BS168_reference_genome | 1,023,150 | C→A | 30.0% | intergenic (+50/‑200) | yhdF → / → bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| RA | BS168_reference_genome | 1,023,175 | C→A | 24.5% | intergenic (+75/‑175) | yhdF → / → bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| RA | BS168_reference_genome | 1,025,078 | A→C | 19.2% | T72P (ACG→CCG) | yhdH → | putative sodium‑dependent transporter |
| RA | BS168_reference_genome | 1,073,325 | C→G | 5.9% | G131G (GGG→GGC) | scoC ← | transcriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family) |
| RA | BS168_reference_genome | 1,083,515 | A→T | 8.2% | I72N (ATT→AAT) | hmoB ← | heme‑degrading monooxygenase |
| RA | BS168_reference_genome | 1,099,980 | G→A | 5.9% | E274E (GAG→GAA) | lplJ → | lipoate‑protein ligase |
| RA | BS168_reference_genome | 1,132,245 | A→C | 16.8% | G380G (GGT→GGG) | yhjO ← | putative permease (recent HGT island) |
| RA | BS168_reference_genome | 1,224,521 | A→G | 100% | E356G (GAA→GGA) | oppD → | oligopeptide ABC transporter (ATP‑binding protein) |
| RA | BS168_reference_genome | 1,254,032 | C→T | 8.5% | R654K (AGG→AAG) | yjcD ← | putative ATP‑dependent DNA helicase (sporulation associated) |
| RA | BS168_reference_genome | 1,317,152:1 | +GT | 100% | intergenic (+23/+5) | phrA → / ← yjpA | secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme |
| RA | BS168_reference_genome | 1,317,153:1 | +T | 100% | intergenic (+24/+4) | phrA → / ← yjpA | secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme |
| RA | BS168_reference_genome | 1,349,495 | G→T | 100% | A325A (GCC→GCA) | pitB ← | putative low‑affinity inorganic phosphate transporter |
| RA | BS168_reference_genome | 1,474,890 | C→T | 100% | L45L (TTG→TTA) | kre ← | regulator of transcription factor ComK function via modulation of mRNA stability |
| RA | BS168_reference_genome | 1,495,070 | T→C | 9.5% | E100G (GAA→GGA) | sppO ← | spore protein cse15 |
| RA | BS168_reference_genome | 1,511,930 | G→T | 29.9% | S88* (TCA→TAA) | ykoA ← | putative membrane protein of unknown function |
| RA | BS168_reference_genome | 1,517,908 | G→A | 5.0% | R15H (CGC→CAC) | abhA → | transcriptional regulator |
| RA | BS168_reference_genome | 1,565,798 | C→A | 19.8% | intergenic (‑89/‑51) | ylbA ← / → ylbB | conserved hypothetical protein/putative enzyme |
| RA | BS168_reference_genome | 1,605,538 | T→A | 100% | intergenic (+48/‑92) | sigE → / → sigG | RNA polymerase sporulation‑specific sigma‑29 factor (sigma‑E)/RNA polymerase sporulation‑specific sigma factor (sigma‑G) |
| RA | BS168_reference_genome | 1,616,570 | C→A | 15.9% | Q102K (CAA→AAA) | ylyA → | sporulation‑related RNA polymerase‑binding factor |
| RA | BS168_reference_genome | 1,690,758 | C→A | 100% | R214S (CGT→AGT) | codY → | transcriptional regulator, GTP and BCAA‑dependent |
| RA | BS168_reference_genome | 1,908,853 | T→G | 20.3% | F454V (TTT→GTT) | yndD → | spore germination receptor subunit |
| RA | BS168_reference_genome | 1,914,659 | C→A | 5.5% | I10I (ATC→ATA) | yndK → | putative phage/plasmid replication protein |
| RA | BS168_reference_genome | 1,914,857 | T→A | 9.1% | I76I (ATT→ATA) | yndK → | putative phage/plasmid replication protein |
| RA | BS168_reference_genome | 1,918,261 | C→A | 20.0% | intergenic (‑5/‑145) | lexA ← / → dinK | transcriptional repressor of the SOS regulon/cell division inhibitor |
| RA | BS168_reference_genome | 1,940,723 | A→C | 22.4% | K33N (AAA→AAC) | eglS → | endo‑1,4‑beta‑glucanase |
| RA | BS168_reference_genome | 1,957,976 | C→A | 26.9% | intergenic (‑57/+51) | BSU_18275 ← / ← yngK | conserved protein of unknown function/putative exported glycoside hydrolase |
| RA | BS168_reference_genome | 1,976,395 | C→A | 5.6% | G2052* (GGA→TGA) | ppsC ← | non‑ribosomal plipastatin synthetase C involved in synthesis of plipastatin |
| RA | BS168_reference_genome | 1,994,711 | C→T | 100% | E1083K (GAA→AAA) | ppsA ← | non‑ribosomal plipastatin synthetase A involved in synthesis of plipastatin |
| RA | BS168_reference_genome | 2,015,087 | C→T | 6.5% | S103S (AGC→AGT) | gltC → | transcriptional regulator (LysR family) (GltC‑glutamate) |
| RA | BS168_reference_genome | 2,021,779 | T→A | 8.4% | N230I (AAC→ATC) | yoaB ← | negatively charged metabolite transporter |
| RA | BS168_reference_genome | 2,043,841 | T→C | 5.4% | L55S (TTA→TCA) | yozG → | putative antitoxin of a toxin / antitoxin system; putative transcriptional regulator |
| RA | BS168_reference_genome | 2,086,527 | G→A | 5.0% | A27V (GCA→GTA) | yozB ← | putative integral inner membrane protein |
| RA | BS168_reference_genome | 2,097,080:1 | +A | 100% | intergenic (‑104/+46) | azoJ ← / ← yocK | FMN‑dependent NADH‑azoreductase/putative general stress protein |
| RA | BS168_reference_genome | 2,119,350 | G→T | 22.1% | intergenic (‑223/‑43) | cdaS ← / → mdtK | spore diadenylate cyclase/Na+‑driven efflux MatE family protein |
| RA | BS168_reference_genome | 2,271,424 | T→C | 100% | R78R (AGA→AGG) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| RA | BS168_reference_genome | 2,271,505 | C→T | 100% | V51V (GTG→GTA) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| RA | BS168_reference_genome | 2,271,523 | A→C | 100% | D45E (GAT→GAG) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| MC JC | BS168_reference_genome | 2,344,013 | Δ39 bp | 100% | coding (2570‑2608/2745 nt) | ponA → | peptidoglycan glycosyltransferase (penicillin‑binding proteins 1A and 1B) |
| RA | BS168_reference_genome | 2,358,909 | C→T | 8.3% | A4T (GCT→ACT) | bshBA ← | N‑acetyl‑alpha‑D‑glucosaminyl L‑malate deacetylase 1; malate N‑acetylglucosamine N‑acetyl hydrolase |
| RA | BS168_reference_genome | 2,358,944 | A→G | 7.4% | R127R (CGT→CGC) | mgsA ← | methylglyoxal synthase |
| RA | BS168_reference_genome | 2,358,977 | C→G | 7.5% | V116V (GTG→GTC) | mgsA ← | methylglyoxal synthase |
| RA | BS168_reference_genome | 2,358,986 | T→C | 7.0% | E113E (GAA→GAG) | mgsA ← | methylglyoxal synthase |
| RA | BS168_reference_genome | 2,364,921 | A→G | 7.5% | L58L (TTG→CTG) | qcrA ← | menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit) |
| RA | BS168_reference_genome | 2,364,928 | A→T | 7.2% | V55V (GTT→GTA) | qcrA ← | menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit) |
| RA | BS168_reference_genome | 2,364,949 | A→G | 8.5% | D48D (GAT→GAC) | qcrA ← | menaquinol:cytochrome c oxidoreductase (iron‑sulfur subunit) |
| RA | BS168_reference_genome | 2,379,904 | A→G | 8.2% | H123H (CAT→CAC) | aroF ← | chorismate synthase |
| RA | BS168_reference_genome | 2,379,919 | T→C | 8.9% | A118A (GCA→GCG) | aroF ← | chorismate synthase |
| RA | BS168_reference_genome | 2,379,922 | A→T | 11.4% | G117G (GGT→GGA) | aroF ← | chorismate synthase |
| RA | BS168_reference_genome | 2,387,878:1 | +A | 100% | coding (711/735 nt) | yphF ← | putative lipoprotein of unknown function |
| RA | BS168_reference_genome | 2,436,674 | A→T | 6.7% | Q210H (CAA→CAT) | ypuA → | putative exported protein |
| RA | BS168_reference_genome | 2,441,380 | T→A | 6.3% | S138C (AGT→TGT) | spoVAD ← | stage V sporulation protein AD (uptake of pyridine‑2,6‑dicarboxylic acid) |
| RA | BS168_reference_genome | 2,476,636 | A→G | 5.2% | Q265R (CAG→CGG) | yqjM → | NADPH‑dependent flavin oxidoreductase (acting on cinnamaldehyde‑related compounds) |
| RA | BS168_reference_genome | 2,480,646 | 2 bp→AT | 100% | intergenic (+21/+103) | zwf → / ← gndA | glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase |
| RA | BS168_reference_genome | 2,480,654 | Δ1 bp | 100% | intergenic (+29/+96) | zwf → / ← gndA | glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase |
| RA | BS168_reference_genome | 2,480,667 | Δ1 bp | 100% | intergenic (+42/+83) | zwf → / ← gndA | glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase |
| RA | BS168_reference_genome | 2,528,711 | T→G | 27.8% | N182T (AAC→ACC) | folD ← | methylenetetrahydrofolate dehydrogenase; methenyltetrahydrofolate cyclohydrolase |
| RA | BS168_reference_genome | 2,568,536 | A→T | 7.0% | intergenic (‑46/+37) | yqgT ← / ← ltaSC | putative d,l‑endopeptidase/enzyme responsible for polyglycerolphosphate LTA synthesis |
| RA | BS168_reference_genome | 2,581,726:1 | +T | 100% | intergenic (‑109/+45) | pstS ← / ← pbpA | phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A) |
| RA | BS168_reference_genome | 2,732,512 | T→C | 7.3% | pseudogene (36/159 nt) | yrdDc ← | hypothetical protein; C‑terminal part of YrdD |
| RA | BS168_reference_genome | 2,772,038 | G→T | 5.1% | A241S (GCC→TCC) | oatA → | peptidoglycan O‑acetyltransferase |
| RA | BS168_reference_genome | 2,772,560 | A→G | 8.9% | T415A (ACC→GCC) | oatA → | peptidoglycan O‑acetyltransferase |
| RA | BS168_reference_genome | 2,818,348 | G→T | 20.6% | P5Q (CCA→CAA) | yrzK ← | conserved protein of unknown function |
| RA | BS168_reference_genome | 2,881,829 | T→C | 9.1% | E321G (GAG→GGG) | lonA ← | class III heat‑shock ATP‑dependent LonA protease |
| RA | BS168_reference_genome | 3,012,504 | A→G | 5.8% | Y211Y (TAT→TAC) | argH ← | argininosuccinate lyase |
| RA | BS168_reference_genome | 3,013,822 | A→G | 6.0% | S175P (TCT→CCT) | argG ← | argininosuccinate synthase |
| RA | BS168_reference_genome | 3,035,116 | A→C | 14.9% | V107G (GTT→GGT) | dgcP ← | diguanylate cyclase |
| RA | BS168_reference_genome | 3,097,107 | G→A | 5.3% | G109D (GGC→GAC) | msmR → | transcriptional regulator (LacI family) |
| RA | BS168_reference_genome | 3,114,257 | A→G | 6.4% | L345L (TTG→CTG) | ytrF ← | metabolite permease involved in resistance to cell wall inhibitors |
| RA | BS168_reference_genome | 3,162,506 | G→T | 6.2% | M141I (ATG→ATT) | cotI → | spore coat kinase |
| RA | BS168_reference_genome | 3,194,626 | T→C | 5.6% | intergenic (‑99/+9) | trnSL‑Ala1 ← / ← uppP | tRNA‑Ala/undecaprenyl‑pyrophosphate phosphatase |
| RA | BS168_reference_genome | 3,363,614 | G→A | 8.3% | L226L (CTC→CTT) | metN ← | methionine ABC transporter (ATP‑binding protein) |
| RA | BS168_reference_genome | 3,412,058 | G→T | 12.6% | L125F (TTG→TTT) | rsiO → | acid stress‑sensitive anti sigma factor |
| RA | BS168_reference_genome | 3,421,976 | A→T | 6.2% | T69S (ACA→TCA) | yvsG → | putative membrane hydrolase |
| RA | BS168_reference_genome | 3,478,630 | T→G | 19.5% | R261S (AGA→AGC) | pgm ← | phosphoglycerate mutase |
| RA | BS168_reference_genome | 3,531,060 | C→G | 24.1% | G142G (GGC→GGG) | pnbA → | para‑nitrobenzyl esterase (intracellular esterase B) |
| RA | BS168_reference_genome | 3,531,092 | A→T | 25.3% | Y153F (TAT→TTT) | pnbA → | para‑nitrobenzyl esterase (intracellular esterase B) |
| RA | BS168_reference_genome | 3,592,481 | T→G | 18.0% | S305S (TCA→TCC) | yvoD ← | putative integral inner membrane protein with nucleotide binding domain |
| RA | BS168_reference_genome | 3,755,776 | T→G | 15.9% | S29R (AGC→CGC) | ywoC ← | putative enzyme of isochorismatase family |
| RA | BS168_reference_genome | 3,766,421 | T→G | 20.3% | S53S (TCA→TCC) | ywnA ← | putative transcriptional regulator |
| RA | BS168_reference_genome | 3,770,058:1 | +A | 100% | intergenic (‑250/+46) | ywzF ← / ← csbD | conserved protein of unknown function/stress response protein |
| RA | BS168_reference_genome | 3,774,612 | C→A | 27.7% | intergenic (‑51/+43) | ywmE ← / ← ywmD | conserved protein of unknown function/putative exported protein |
| RA | BS168_reference_genome | 3,831,264 | C→T | 5.3% | N168N (AAC→AAT) | ywiC → | putative integral inner membrane protein |
| RA | BS168_reference_genome | 3,907,379 | A→C | 30.9% | intergenic (‑65/‑465) | ywcI ← / → vpr | conserved protein of unknown function/extracellular serine protease |
| RA | BS168_reference_genome | 3,935,823 | Δ1 bp | 100% | intergenic (+35/+1) | ywbE → / ← rlmI | conserved protein of unknown function/large subunit ribosomal RNA m5C1962 methyltransferase |
| RA | BS168_reference_genome | 4,019,488 | A→C | 17.1% | L41R (CTT→CGT) | yxiK ← | conserved protein of unknown function |
| RA | BS168_reference_genome | 4,020,611 | A→C | 16.5% | S99S (TCT→TCG) | yxzG ← | putative nucleic acid binding protein |
| RA | BS168_reference_genome | 4,028,640 | C→A | 7.1% | G637* (GGA→TGA) | wapA ← | cell wall‑associated tRNA nuclease precursor; intercellular growth inhibitor |
| RA | BS168_reference_genome | 4,153,115 | G→T | 100% | L192I (CTT→ATT) | walK ← | two‑component sensor histidine kinase [YycG] |
| RA | BS168_reference_genome | 4,155,390:1 | +A | 100% | intergenic (‑181/+43) | trnY‑Lys ← / ← purA | tRNA‑Lys/adenylosuccinate synthetase |
| RA | Exported | 121 | G→C | 5.6% | intergenic (–/‑2857) | – / → eryR | –//vntifkey=4 |
| RA | Exported | 131 | G→C | 6.7% | intergenic (–/‑2847) | – / → eryR | –//vntifkey=4 |
| RA | Exported | 2,179 | Δ1 bp | 100% | intergenic (–/‑799) | – / → eryR | –//vntifkey=4 |
| JC | Exported | 2,655 | Δ3 bp | 100% | intergenic (–/‑321) | – / → eryR | –//vntifkey=4 |
| RA | Exported | 3,729 | T→G | 7.8% | intergenic (+14/‑142) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,735 | A→T | 11.1% | intergenic (+20/‑136) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,741 | G→T | 11.2% | intergenic (+26/‑130) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,743 | A→T | 6.4% | intergenic (+28/‑128) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,749 | A→T | 6.3% | intergenic (+34/‑122) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,750 | C→A | 5.6% | intergenic (+35/‑121) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 3,783 | G→T | 6.0% | intergenic (+68/‑88) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| JC | Exported | 3,843 | Δ5 bp | 100% | intergenic (+128/‑24) | eryR → / → ampR | /vntifkey=4//vntifkey=4 |
| RA | Exported | 4,625 | A→C | 6.5% | K252T (AAG→ACG) | ampR → | /vntifkey=4 |
| RA | Exported | 4,648 | T→A | 10.6% | Y260N (TAC→AAC) ‡ | ampR → | /vntifkey=4 |
| RA | Exported | 4,649 | A→C | 8.8% | Y260S (TAC→TCC) ‡ | ampR → | /vntifkey=4 |
| RA | Exported | 5,049 | C→T | 100% | intergenic (+318/‑839) | ampR → / → lacZalpha | /vntifkey=4//vntifkey=4 |
| RA | Exported | 5,173 | T→G | 6.4% | intergenic (+442/‑715) | ampR → / → lacZalpha | /vntifkey=4//vntifkey=4 |
| RA | Exported | 5,464 | T→G | 5.4% | intergenic (+733/‑424) | ampR → / → lacZalpha | /vntifkey=4//vntifkey=4 |
| RA | Exported | 6,006 | T→G | 6.0% | V40G (GTT→GGT) | lacZalpha → | /vntifkey=4 |
| RA | Exported | 6,031 | A→C | 6.7% | A48A (GCA→GCC) | lacZalpha → | /vntifkey=4 |
| RA | Exported | 6,033 | A→C | 5.3% | H49P (CAT→CCT) | lacZalpha → | /vntifkey=4 |
| RA | Exported | 6,047 | A→C | 5.4% | S54R (AGC→CGC) | lacZalpha → | /vntifkey=4 |
| RA | Exported | 6,256 | A→C | 5.9% | intergenic (+45/–) | lacZalpha → / – | /vntifkey=4/– |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | BS168_reference_genome | 160964–164898 | 165619–164903 | 6–4656 | 21 [15] | [19] 20 | [rrnI‑16S]–[rrnI‑5S] | [rrnI‑16S],rrnI‑23S,[rrnI‑5S] |
| * | * | ÷ | BS168_reference_genome | 166444–166813 | 171227–170391 | 3579–4784 | 21 [18] | [17] 24 | rrnH‑16S–[rrnH‑5S] | rrnH‑16S,rrnH‑23S,[rrnH‑5S] |
| * | * | ÷ | BS168_reference_genome | 171467–171679 | 176227–172978 | 1300–4761 | 21 [18] | [17] 20 | rrnG‑16S–[rrnG‑5S] | rrnG‑16S,rrnG‑23S,[rrnG‑5S] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | BS168_reference_genome | 2152031 = | 73 (0.990) | 5 (0.080) | 4/200 | NT | 7.5% | pseudogene (406/420 nt) | spsMn | spore capsular polysaccharide biosynthesis enzyme fragment; N‑terminal part of SpsM |
| ? | BS168_reference_genome | = 2286432 | 60 (0.940) | intergenic (+24/‑16) | sprA/spsMc | serine‑type phage integrase; phage SPbeta/sporulation capsular polysaccharide biosynthesis enzyme C‑terminal end; restored upon phage SPbeta excision | |||||