breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 278,744 +A intergenic (‑239/+75) PP_0224 ← / ← sctC DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein
RA 307,866 +C intergenic (+881/‑988) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein
RA 336,125 +T coding (210/237 nt) PP_0278 → hypothetical protein
RA 353,067 A→G intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
RA 499,203 A→G intergenic (+47/‑249) uhpA → / → PP_0411 two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
RA 1,070,248 +GA intergenic (+48/+94) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
RA 1,095,512 +A coding (549/1494 nt) rpoN ← RNA polymerase sigma‑54 factor
RA 1,126,647 +C intergenic (‑101/+48) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase
RA 1,212,521 C→T intergenic (‑55/+133) PP_1060 ← / ← PP_1061 glutamate synthase large subunit/ATP‑dependent DNA helicase
RA 1,419,549 +C intergenic (‑38/+98) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein
RA 1,499,480 2 bp→AC intergenic (+57/‑106) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,499,508 +C intergenic (+85/‑79) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
RA 1,581,266 G→T A44D (GCC→GAC)  ttgB ← efflux pump membrane protein TtgB
RA 1,932,640 +C intergenic (‑107/+43) rluA ← / ← minE bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
RA 2,202,220 G→A intergenic (+77/‑104) PP_1946 → / → PP_1947 short‑chain dehydrogenase/reductase family oxidoreductase/hypothetical protein
RA 2,722,068 C→T Q197* (CAG→TAG)  PP_2382 → putative transporter
RA 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin
RA 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
RA 3,951,159 T→C intergenic (‑123/+72) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 3,951,161 A→T intergenic (‑125/+70) PP_3486 ← / ← PP_3487 cytochrome c/hypothetical protein
RA 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,035 +G intergenic (+118/+97) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,586,057 +C intergenic (+140/+75) PP_4061 → / ← PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
RA 4,718,040 T→A M202K (ATG→AAG)  fabB → 3‑oxoacyl‑ACP synthase
RA 4,740,811 (T)7→6 intergenic (+48/+705) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,740,819 2 bp→CT intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,229 A→C intergenic (+466/+287) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,231 C→G intergenic (+468/+285) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,234 C→T intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,236 2 bp→TG intergenic (+473/+279) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,239 A→G intergenic (+476/+277) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,243 3 bp→TGC intergenic (+480/+271) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,255 C→T intergenic (+492/+261) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,257 A→C intergenic (+494/+259) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,260 2 bp→GC intergenic (+497/+255) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,263 A→C intergenic (+500/+253) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,272 A→G intergenic (+509/+244) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,274 A→C intergenic (+511/+242) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,276 G→C intergenic (+513/+240) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,278 C→T intergenic (+515/+238) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,283 +T intergenic (+520/+233) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,289 C→G intergenic (+526/+227) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,292 C→T intergenic (+529/+224) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,294 C→G intergenic (+531/+222) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,296 A→C intergenic (+533/+220) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,741,298 C→T intergenic (+535/+218) gltA → / ← yeiW citrate synthase/putative oxidoreductase
JC 4,980,585 +GGC intergenic (‑24/+44) PP_4387 ← / ← flgE hypothetical protein/flagellar hook protein FlgE
JC 5,555,608 +GCC intergenic (‑84/+87) PP_4887 ← / ← PP_4888 hypothetical protein/methyl‑accepting chemotaxis transducer
RA 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
RA 5,674,753 G→C intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
JC 5,681,415 +CGGG intergenic (‑133/+71) PP_4986 ← / ← PP_4987 hemolysin III family channel protein/putative Chemotaxis protein
RA 5,947,807 C→A V223L (GTG→TTG)  rho ← transcription termination factor Rho
RA 5,986,841 T→A intergenic (+121/‑78) PP_5245 → / → kefB‑III AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
RA 6,013,885 T→C intergenic (+90/+20) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
RA 6,150,396 C→T intergenic (‑2580/‑594) PP_5393 ← / → PP_5395 metal‑binding chaperone/hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171386–176276 176276 1–4891 8 [6] [6] 7 PP_16SA–[PP_5SA] PP_16SA,PP_23SA,[PP_5SA]
* * ÷ NC_002947 176666–181522 181522 1–4857 7 [6] [6] 7 PP_16SB–[PP_23SB] PP_16SB,[PP_23SB]
* * ÷ NC_002947 1124526 1124615 90 7 [6] [6] 7 gcvT‑I aminomethyltransferase
* * ÷ NC_002947 2299501 2299618 118 8 [6] [6] 7 sbcC exonuclease subunit SbcC
* * ÷ NC_002947 4808180 4808230 51 13 [0] [6] 7 PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* * ÷ NC_002947 5525440–5525474 5525539 66–100 7 [5] [4] 7 PP_4858 hypothetical protein
* * ÷ NC_002947 5716209 5716350 142 7 [5] [6] 7 tatC‑II Sec‑independent protein translocase protein
* * ÷ NC_002947 6098363 6098486 124 7 [6] [6] 7 PP_5350 RpiR family transcriptional regulator

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 1816560 (0.000)6 (0.210) 6/278 2.7 100% intergenic (+122/‑11) PP_23SB/PP_r01 23S ribosomal RNA/5S ribosomal RNA
?NC_002947 = 1330343 NA (NA)intergenic (+125/‑7) PP_23SE/PP_5SE 23S ribosomal RNA/5S ribosomal RNA
* ? NC_002947 1440000 =NA (NA)32 (1.090) 31/280 0.1 100% intergenic (‑331/‑328) yveA/PP_1260 aspartate‑proton symporter/transposase
?NC_002947 = 1844451 0 (0.000)coding (343/2754 nt) gacS sensor protein GacS
* ? NC_002947 = 1441693NA (NA)31 (1.040) 24/284 0.3 100% intergenic (+37/+395) PP_1260/ghrB transposase/2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase
?NC_002947 1844449 = 0 (0.000)coding (345/2754 nt) gacS sensor protein GacS
* ? NC_002947 2301453 =0 (0.000)31 (1.040) 25/284 0.3 100% intergenic (‑97/‑234) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 2925429 = NA (NA)intergenic (‑177/+388) PP_2563/PP_2564 antibiotic biosynthesis protein/quercetin 2,3‑dioxygenase
* ? NC_002947 = 23014540 (0.000)31 (1.040) 26/286 0.3 100% intergenic (‑98/‑233) sbcD/PP_5497 exonuclease subunit SbcD/rhs repeat‑associated core domain‑containing protein
?NC_002947 5545321 = NA (NA)intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
* ? NC_002947 = 295708022 (0.730)6 (0.210) 6/280 2.7 21.3% intergenic (+35/‑161) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
?NC_002947 2957165 = 23 (0.790)intergenic (+120/‑76) PP_2588/PP_2589 class III aminotransferase/aldehyde dehydrogenase family protein
* ? NC_002947 = 48081790 (0.000)5 (0.190) 5/250 2.7 30.3% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5988949 23 (0.880)intergenic (+243/‑303) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 5545321 =NA (NA)37 (1.240) 29/286 0.2 97.4% intergenic (‑461/+416) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NC_002947 = 5621245 1 (0.030)coding (19/774 nt) PP_4939 hypothetical protein
* ? NC_002947 = 5545666NA (NA)46 (1.550) 31/284 0.1 97.9% intergenic (‑806/+71) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
?NC_002947 5621244 = 1 (0.030)coding (20/774 nt) PP_4939 hypothetical protein