breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A
RA CP000731 6,310 A→T 6.6% intergenic (+827/‑661) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
JC CP000731 6,552 +28 bp 100% intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 25,189 C→A 5.3% intergenic (+87/‑159) USA300HOU_pUSA300HOUMR0030 → / → USA300HOU_pUSA300HOUMR0031 Sin recombinase/hypothetical protein
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
JC USA300TCH1516_ALE 68,656 Δ3 bp 5.4% coding (772‑774/852 nt) USA300TCH1516_00061 → hypothetical protein
RA USA300TCH1516_ALE 75,007 C→T 7.9% M1M (ATG→ATA) † argR_1 ← Arginine repressor
RA USA300TCH1516_ALE 110,651 C→T 12.8% D170D (GAC→GAT USA300HOU_RS00515 → putative lipoprotein
RA USA300TCH1516_ALE 110,660 T→A 17.0% I173I (ATT→ATA USA300HOU_RS00515 → putative lipoprotein
RA USA300TCH1516_ALE 111,512 A→G 24.6% S194S (TCA→TCG USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 111,516 C→A 24.3% Q196K (CAA→AAA)  USA300HOU_RS00520 → putative lipoprotein
RA USA300TCH1516_ALE 235,092 C→T 6.5% D490N (GAT→AAT)  gsiA ← Glutathione import ATP‑binding protein GsiA
RA USA300TCH1516_ALE 293,940 C→T 19.6% intergenic (+57/‑471) gatD → / → tarI' Galactitol‑1‑phosphate 5‑dehydrogenase/Ribitol‑5‑phosphate cytidylyltransferase 2
RA USA300TCH1516_ALE 317,291 A→G 5.3% M192T (ATG→ACG)  degA ← HTH‑type transcriptional regulator DegA
RA USA300TCH1516_ALE 478,502 C→A 100% D342E (GAC→GAA nuoL → NADH‑quinone oxidoreductase subunit L
RA USA300TCH1516_ALE 512,643 A→G 45.8% intergenic (+711/‑6052) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase
RA USA300TCH1516_ALE 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ 50S ribosomal protein L1/50S ribosomal protein L10
RA USA300TCH1516_ALE 622,479 T→C 10.0% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E
RA USA300TCH1516_ALE 628,618 T→A 6.9% S8T (TCG→ACG)  nagB_1 → Glucosamine‑6‑phosphate deaminase
RA USA300TCH1516_ALE 677,733 A→G 5.2% I204V (ATT→GTT)  USA300HOU_RS03265 → hypothetical protein
RA USA300TCH1516_ALE 685,779 T→C 8.7% L127L (TTA→TTG USA300HOU_RS03310 ← hypothetical protein
RA USA300TCH1516_ALE 707,909 A→T 100% M1M (TTG→ATG) † znuC_1 ← High‑affinity zinc uptake system ATP‑binding protein ZnuC
RA USA300TCH1516_ALE 973,213 C→A 100% E175* (GAG→TAG)  yitU_1 ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase YitU
RA USA300TCH1516_ALE 1,002,200 T→G 100% M173R (ATG→AGG)  mecA → Adapter protein MecA 1
RA USA300TCH1516_ALE 1,105,994 T→C 100% T12T (ACT→ACC pdhD → Dihydrolipoyl dehydrogenase
RA USA300TCH1516_ALE 1,133,417 T→A 5.7% V146V (GTT→GTA rsmD → Ribosomal RNA small subunit methyltransferase D
RA USA300TCH1516_ALE 1,134,851 A→T 5.4% I119I (ATT→ATA USA300HOU_RS05620 ← Nicotinamide‑nucleotide adenylyltransferase
RA USA300TCH1516_ALE 1,196,508 C→A 100% S338Y (TCT→TAT)  ftsZ → Cell division protein FtsZ
RA USA300TCH1516_ALE 1,249,530 G→A 5.7% A956T (GCG→ACG)  smc_2 → Chromosome partition protein Smc
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_RS07505 Chorismate synthase/hypothetical protein
RA USA300TCH1516_ALE 1,617,132 C→T 5.6% L212L (CTC→CTT yhdN_2 → General stress protein 69
RA USA300TCH1516_ALE 1,620,134 C→T 8.1% A163A (GCG→GCA rnz ← Ribonuclease Z
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← Adenine phosphoribosyltransferase
RA USA300TCH1516_ALE 1,830,450 T→C 7.2% T224A (ACT→GCT)  ezrA ← Septation ring formation regulator EzrA
RA USA300TCH1516_ALE 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,184,114 A→G 5.3% R277R (CGT→CGC rpsA_2 ← 30S ribosomal protein S1
RA USA300TCH1516_ALE 2,274,436 A→G 5.0% T454T (ACA→ACG mtlA → PTS system mannitol‑specific EIICB component
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,337,959 C→T 100% intergenic (‑125/‑283) adhR ← / → USA300HOU_RS11940 HTH‑type transcriptional regulator AdhR/Hyaluronate lyase
RA USA300TCH1516_ALE 2,343,438 C→T 33.3% intergenic (+114/+250) USA300HOU_RS11955 → / ← aldC_1 65 kDa membrane protein/Alpha‑acetolactate decarboxylase
RA USA300TCH1516_ALE 2,455,692 Δ1 bp 100% coding (1210/1260 nt) lyrA → Lysostaphin resistance protein A
RA USA300TCH1516_ALE 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) lyrA → Lysostaphin resistance protein A
RA USA300TCH1516_ALE 2,468,887 A→T 5.6% intergenic (‑76/‑132) lipY ← / → USA300HOU_RS12715 Triacylglycerol lipase/hypothetical protein
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,771,347 C→A 5.9% P49Q (CCA→CAA)  phoB → Alkaline phosphatase 3
RA USA300TCH1516_ALE 2,774,969 A→G 6.8% D739D (GAT→GAC clfB ← Clumping factor B

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 512620 =102 (1.350)57 (1.090)
+40 bp
32/180 NT 44.8% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
* ? USA300TCH1516_ALE 895785 =50 (0.660)10 (0.150) 8/226 NT 18.3% coding (806/822 nt) metQ_2 Methionine‑binding lipoprotein MetQ
?USA300TCH1516_ALE = 909740 46 (0.700)intergenic (‑663/‑371) USA300HOU_RS04500/csbD_1 hypothetical protein/Stress response protein CsbD
* ? USA300TCH1516_ALE 2085296 =49 (0.650)19 (0.280) 14/232 NT 26.1% coding (173/201 nt) hlb_1 Phospholipase C
?USA300TCH1516_ALE = 2128343 64 (0.950)coding (1/825 nt) hlb_2 Phospholipase C
* ? USA300TCH1516_ALE = 241989790 (1.190)5 (0.080) 4/228 NT 5.5% intergenic (+65/‑172) USA300HOU_RS12460/ssaA2_3 hypothetical protein/Staphylococcal secretory antigen ssaA2
?USA300TCH1516_ALE = 2419903 92 (1.390)intergenic (+71/‑166) USA300HOU_RS12460/ssaA2_3 hypothetical protein/Staphylococcal secretory antigen ssaA2