Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
14 |
24.7 |
1576304 |
96.5% |
1521133 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,875,632 |
(T)7→8 |
intergenic (‑66/+287) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,875,625 | 1 | . | T | 88.5%
| 84.4
/ 6.7
| 26 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (12/11); total (13/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 |
GCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875553‑3875695
|
gCATTATTCAGCATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt < 2:8371/76‑1 (MQ=255)
ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttt > 2:433350/1‑76 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttt‑tt > 1:3937/1‑75 (MQ=255)
ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttt‑ttc < 1:629987/76‑3 (MQ=255)
cAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTT‑TCATc < 2:402827/76‑1 (MQ=255)
aCATACGCGTCCCGTCTCTAATTGNAGAACGAATTTAAACCGCTTCACCTTGAAACAANTACATTTTTTT‑TCATCg < 2:655227/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTg > 1:308010/1‑76 (MQ=255)
cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGaaa < 1:520278/76‑1 (MQ=255)
gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATgg < 1:143576/76‑1 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACa < 1:182962/76‑1 (MQ=255)
gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAACAACTACATTTTTTT‑TCATCGTGAAATGGACaga > 2:644559/1‑76 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGAt > 2:595772/1‑76 (MQ=255)
tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATa < 1:13486/76‑1 (MQ=255)
tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAAt < 2:353580/76‑1 (MQ=255)
gagaACGAATTTAAACCGCTTCACNTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGt > 2:134522/1‑76 (MQ=255)
agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGtt > 2:530467/1‑76 (MQ=255)
agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGtt > 1:225827/1‑76 (MQ=255)
cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAAt > 2:339211/1‑76 (MQ=255)
gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAAATGTCCAATa < 2:87008/76‑1 (MQ=255)
aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc > 1:412346/1‑76 (MQ=255)
aTTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 2:208188/1‑76 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 1:646766/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 2:539509/76‑1 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:311084/76‑1 (MQ=255)
tCACCTAGAAAAAACTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 1:121236/1‑76 (MQ=255)
ccTTGAGAAAACTACA‑TTTTTTNTCATCGTGAANTGGACAGANGATAAATTGTTCAATAACTGATNATCCCCGCTg < 2:686044/76‑1 (MQ=255)
aaaaaCTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCACTGACGaaa < 2:397560/76‑1 (MQ=255)
aaaCTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:150936/1‑76 (MQ=255)
aaCTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg > 2:21511/1‑76 (MQ=255)
cTACATTTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc < 1:45330/76‑1 (MQ=255)
|
GCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875553‑3875695
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
