Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A47 F1 I1 R1
|
17 |
69.1 |
4502028 |
96.3% |
4335452 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,875,632 |
(T)7→8 |
intergenic (‑66/+287) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,875,625 | 1 | . | T | 98.6%
| 279.0
/ ‑3.4
| 70 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (37/32); total (38/32) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875551‑3875695
|
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑t > 2:813112/1‑76 (MQ=255)
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑t > 1:279285/1‑76 (MQ=255)
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑t < 1:1580027/76‑1 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑tttttttt > 2:1789456/1‑75 (MQ=255)
cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGt < 1:2062349/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGTAAAAACTA‑CATTTTTTTTCATCGTg > 1:2130896/1‑76 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTg < 2:1802548/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTg < 1:57479/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTg < 2:1812677/76‑1 (MQ=255)
aaCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGa < 2:1512287/75‑1 (MQ=255)
cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGaaa > 2:536832/1‑76 (MQ=255)
gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAAt < 1:1584818/76‑1 (MQ=255)
cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATg > 1:299916/1‑76 (MQ=255)
gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATgg < 1:425890/76‑1 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATgta > 1:1870220/1‑74 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGa > 1:569616/1‑76 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGa > 1:569633/1‑76 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGa < 1:802314/76‑1 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGAc > 1:533485/1‑76 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGAc < 1:1575426/76‑1 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGAc < 1:1880262/76‑1 (MQ=255)
tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACag < 2:886648/76‑1 (MQ=255)
tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACag < 1:1115023/76‑1 (MQ=255)
aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACagag > 1:957182/1‑76 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGAt < 1:1706358/76‑1 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGAt < 2:361325/76‑1 (MQ=255)
tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATa < 1:1287699/76‑1 (MQ=255)
aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATaa < 1:1432097/76‑1 (MQ=255)
aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATaaa < 1:813112/76‑1 (MQ=255)
aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATaaa > 1:2193781/1‑76 (MQ=255)
ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAAtt < 2:434195/76‑1 (MQ=255)
ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAAtt > 1:1202020/1‑76 (MQ=255)
tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTg > 2:1097174/1‑76 (MQ=255)
gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGt > 2:2517/1‑76 (MQ=255)
gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGt > 2:1285551/1‑76 (MQ=255)
aCGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 2:1901370/76‑1 (MQ=255)
aCGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa < 2:835722/76‑1 (MQ=255)
gAATTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:1033542/1‑76 (MQ=255)
aaTTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 2:490851/76‑1 (MQ=255)
aaTTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 2:1268569/1‑76 (MQ=255)
aTTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc > 1:1532511/1‑76 (MQ=255)
aTTTAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc < 1:1773381/76‑1 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 2:16549/76‑1 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 1:2005409/1‑76 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 1:827859/1‑76 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 1:1906734/76‑1 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg > 1:380340/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:1312222/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:333501/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa < 1:121923/76‑1 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTACCT‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 2:6029/1‑76 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:1403780/67‑1 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCa > 2:1403780/1‑67 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:1602970/76‑1 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 2:254935/1‑76 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 1:1602955/76‑1 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 1:249815/1‑76 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 1:249786/1‑76 (MQ=255)
ccGCTTCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 2:1514325/76‑1 (MQ=255)
ttCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 1:1069945/1‑76 (MQ=255)
ttCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 1:1032425/1‑76 (MQ=255)
ttCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 2:1913319/1‑76 (MQ=255)
tCACCTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 1:786776/1‑76 (MQ=255)
cTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 2:339293/76‑1 (MQ=255)
cTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa < 1:1411333/76‑1 (MQ=255)
cTTGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:625728/1‑76 (MQ=255)
tGAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:5790/1‑76 (MQ=255)
gAAAAAACTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 1:1526849/76‑1 (MQ=255)
aaaaaCTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:512749/1‑76 (MQ=255)
aaaaaCTA‑CATTTTTTTTCATAGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:434576/1‑76 (MQ=255)
aaaaCTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 1:1930807/1‑76 (MQ=255)
aaaCTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:473295/1‑76 (MQ=255)
aaaCTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 2:13662/1‑76 (MQ=255)
cTA‑CATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc > 1:234668/1‑76 (MQ=255)
|
TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTA‑CA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC > REL606/3875551‑3875695
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
