Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A107 F1 I1 R1	19	326.9	20855348	93.1%	19416328	75.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)_7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	99.2%	1010.6 / ‑0.2	244	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (92/150); total (93/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

Only 100 of 294 total aligned reads displayed.

ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875550‑3875695

  aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt                                                                         <  1:10413277/76‑1 (MQ=255)

  aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt                                                                         >  2:6167216/1‑76 (MQ=255)

   gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt                                                                        >  2:5640878/1‑76 (MQ=255)

    cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t                                                                      >  1:6013548/1‑76 (MQ=255)

    cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t                                                                      >  1:9609362/1‑76 (MQ=255)

     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t                                                                    <  1:4055925/76‑1 (MQ=255)

     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t                                                                    <  1:6571098/76‑1 (MQ=255)

     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t                                                                    <  1:978287/76‑1 (MQ=255)

      ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑t                                                                  >  2:69636/1‑76 (MQ=255)

       tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt                                                                 >  1:6357123/1‑76 (MQ=255)

       tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt                                                                 >  2:5514527/1‑76 (MQ=255)

       tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt                                                                 >  1:1168278/1‑76 (MQ=255)

       tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tt                                                                 >  2:454080/1‑76 (MQ=255)

        attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑ttt                                                                <  2:5239783/76‑2 (MQ=255)

          tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑t‑t‑tttca                                                              >  2:9104628/1‑73 (MQ=255)

              cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt                                                           <  2:4786439/76‑1 (MQ=255)

              cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt                                                           >  2:3182469/1‑76 (MQ=255)

              cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGt                                                           <  2:1207176/76‑1 (MQ=255)

               aTACGCGTCCTGACTGTAATTTGAGAACGACTTTAAACCGCTTCACCTTGCAAAAACTACATTTT‑T‑T‑TTCATCGTg                                                          >  1:7479978/1‑76 (MQ=255)

               aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTg                                                          >  2:7244359/1‑76 (MQ=255)

                tACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGa                                                         <  1:5936167/76‑1 (MQ=255)

                 aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaa                                                        <  1:5480784/76‑1 (MQ=255)

                 aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaa                                                        >  1:3654894/1‑76 (MQ=255)

                  cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaaa                                                       <  1:4283006/76‑1 (MQ=255)

                  cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGaaa                                                       <  2:4314908/76‑1 (MQ=255)

                   gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAAt                                                      <  1:7140548/76‑1 (MQ=255)

                    cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATg                                                     >  2:3527712/1‑76 (MQ=255)

                     gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg                                                    >  2:4081149/1‑76 (MQ=255)

                     gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg                                                    <  2:7877368/76‑1 (MQ=255)

                     gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATgg                                                    <  1:1481699/76‑1 (MQ=255)

                      tCCTGACTGTAATTTGAGAACGCATTTAACCCGCTTGACCTTGACAAAACTCCC‑TTT‑TGT‑TTCACCGCGAAACggc                                                   >  2:4471435/1‑75 (MQ=255)

                      tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGa                                                   <  1:2419200/76‑1 (MQ=255)

                      tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGa                                                   >  1:2744231/1‑76 (MQ=255)

                       ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGAc                                                  <  2:6247381/76‑1 (MQ=255)

                        cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACa                                                 >  1:6476016/1‑76 (MQ=255)

                         tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag                                                <  2:185289/76‑1 (MQ=255)

                         tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag                                                <  1:1604620/76‑1 (MQ=255)

                         tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACag                                                <  1:256078/76‑1 (MQ=255)

                            cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAgg                                             >  2:3408013/1‑76 (MQ=255)

                            cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTCT‑T‑TTCATCGTGAAATGGACAGAgg                                             >  2:9252263/1‑76 (MQ=255)

                             tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa                                            >  1:4587015/1‑76 (MQ=255)

                             tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa                                            >  2:8177451/1‑76 (MQ=255)

                             tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGa                                            <  2:8974893/76‑1 (MQ=255)

                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa                                          >  1:10287152/1‑76 (MQ=255)

                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa                                          <  1:9480031/76‑1 (MQ=255)

                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATa                                          <  1:3740391/76‑1 (MQ=255)

                               tAATTTGAGAACGAAGTTAAAACGATTAACCTAGAAAAATCTACA‑TTT‑T‑TATTCATCGTGAAATGAACAGAGGAtt                                          <  2:7701587/76‑2 (MQ=255)

                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa                                         <  2:282017/76‑1 (MQ=255)

                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa                                         >  2:2690739/1‑76 (MQ=255)

                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa                                         <  1:725897/76‑1 (MQ=255)

                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaa                                         >  1:4769033/1‑76 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        <  2:2139223/76‑1 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        <  2:3695944/76‑1 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        >  1:9457578/1‑76 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        >  1:10066411/1‑76 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        <  1:3219951/76‑1 (MQ=255)

                                 aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATaaa                                        <  1:5128445/76‑1 (MQ=255)

                                  tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt                                       <  2:2934537/76‑1 (MQ=255)

                                  tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt                                       >  1:6109375/1‑76 (MQ=255)

                                  tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAt                                       >  1:393814/1‑76 (MQ=255)

                                   ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAAtt                                      <  1:822230/76‑1 (MQ=255)

                                    tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACa                                                 >  2:8820499/1‑64 (MQ=255)

                                    tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTg                                     <  2:10165822/76‑1 (MQ=255)

                                    tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTg                                     <  2:7035716/76‑1 (MQ=255)

                                    tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGt                                    <  1:454080/76‑1 (MQ=255)

                                     gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGt                                    <  1:5514527/76‑1 (MQ=255)

                                       gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTc                                  <  1:3645992/76‑1 (MQ=255)

                                       gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTc                                  <  2:7560156/76‑1 (MQ=255)

                                        aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCa                                 <  1:2287859/76‑1 (MQ=255)

                                         aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                                <  2:334837/76‑1 (MQ=255)

                                          cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:9237484/76‑1 (MQ=255)

                                          cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:4345248/76‑1 (MQ=255)

                                           gAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATa                              <  1:1355998/76‑1 (MQ=255)

                                            aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa                             <  2:4194806/76‑1 (MQ=255)

                                            aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa                             <  2:9329051/76‑1 (MQ=255)

                                              tttAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg                           >  1:8863510/1‑76 (MQ=255)

                                               ttAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg                          >  1:5209125/1‑76 (MQ=255)

                                                tAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                         >  1:5267141/1‑76 (MQ=255)

                                                tAAACCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                         <  1:7382229/76‑1 (MQ=255)

                                                 aaaCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        <  1:1001715/76‑1 (MQ=255)

                                                  aaCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  1:8602736/76‑1 (MQ=255)

                                                   aCCGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa                      <  2:10310410/76‑1 (MQ=255)

                                                    ccGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                     >  1:7647487/1‑76 (MQ=255)

                                                    ccGCTTCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                     <  1:6943555/76‑1 (MQ=255)

                                                         tCACCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                >  2:1480008/1‑76 (MQ=255)

                                                           aCCTTGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt              >  2:5911457/1‑76 (MQ=255)

                                                              ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           <  1:8735555/76‑1 (MQ=255)

                                                              ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           <  1:8996030/76‑1 (MQ=255)

                                                              ttGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           <  1:1944207/76‑1 (MQ=255)

                                                               tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:8358406/76‑1 (MQ=255)

                                                               tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:9357934/76‑1 (MQ=255)

                                                               tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:9972811/76‑1 (MQ=255)

                                                               tGAAAAAACTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  1:294509/76‑1 (MQ=255)

                                                                 aaaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa        <  1:7922833/76‑1 (MQ=255)

                                                                 aaaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa        <  1:3018306/76‑1 (MQ=255)

                                                                  aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       <  2:6653174/76‑1 (MQ=255)

                                                                  aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       >  2:5386323/1‑76 (MQ=255)

                                                                  aaaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       <  2:8432852/76‑1 (MQ=255)

                                                                   aaaaCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      >  2:9215544/1‑76 (MQ=255)

                                                                      aCTACATTTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc   <  2:4816368/76‑1 (MQ=255)

ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑T‑T‑TTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875550‑3875695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A