Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
15 |
61.9 |
4096487 |
94.5% |
3871180 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,875,632 |
(T)7→8 |
intergenic (‑66/+287) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,875,625 | 1 | . | T | 91.9%
| 219.1
/ 10.5
| 62 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (2/3); new base T (30/27); total (32/30) |
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG > REL606/3875552‑3875691
|
aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t < 1:634045/76‑1 (MQ=255)
gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t < 2:1898896/76‑1 (MQ=255)
gCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t < 2:829947/76‑1 (MQ=255)
ttatcCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑t > 1:1880082/1‑76 (MQ=255)
tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑tt > 2:1539305/1‑76 (MQ=255)
ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t‑t‑t‑t‑t‑tttc > 1:416733/1‑74 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTg < 2:846339/76‑1 (MQ=255)
gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAAt < 2:839211/76‑1 (MQ=255)
gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATgg > 1:871474/1‑76 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAATCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAc < 2:678117/76‑1 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCGCCTTGAAAAAACTACA‑T‑T‑TAT‑T‑TT‑CATCGTGAAGTgggc < 2:1971321/76‑3 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATGGTGAAATGGAc < 1:68454/76‑1 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAc < 2:1391776/76‑1 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACa < 2:1038915/76‑1 (MQ=255)
tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACag > 1:630523/1‑76 (MQ=255)
tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGGGAAATGGACag < 1:50776/76‑1 (MQ=255)
gACTGTAATTAGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATTGTGAAATGGACaga > 1:169440/1‑76 (MQ=255)
gTAATTTGAGAANGANTTTAAACCGCTTCACCTTNAAAAAAATACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt < 1:1665932/76‑1 (MQ=255)
gTAATTTGAGAACGAATTTCAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt > 1:647291/1‑76 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt > 1:230586/1‑76 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAt < 1:861498/76‑1 (MQ=255)
tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGAtn < 1:1543004/76‑2 (MQ=255)
aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATaa > 2:1077671/1‑76 (MQ=255)
tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAt < 1:1005006/76‑1 (MQ=255)
tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAt > 1:1026807/1‑76 (MQ=255)
ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACG‑TAT‑T‑T‑T‑TA‑CAACGTGAAATGGAGAGANGATAAAtt > 2:341396/1‑76 (MQ=255)
ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAAtt < 1:497729/76‑1 (MQ=255)
tGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTg < 1:976477/76‑1 (MQ=255)
aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:877572/76‑1 (MQ=255)
aaCGAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCa < 1:1531277/76‑1 (MQ=255)
cGAATTTAAAGCGCTTCGCCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGTATCAATTGTTCAAt > 1:163009/1‑76 (MQ=255)
gAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATa > 1:1053373/1‑76 (MQ=255)
gAATTTAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATa < 1:1240991/76‑1 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg < 1:596199/76‑1 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 2:1417176/1‑76 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg < 2:1772500/76‑1 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TTCCATCGTGAAATGGACAGAAGATAAATTGTTCAATAACgg > 2:1632389/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:809015/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:1417586/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 1:1491446/1‑76 (MQ=255)
aaaCCGCTTCACCTTGAAACAACTACA‑T‑TAT‑T‑T‑AT‑CATAGTGAAGTGGGTAGAGGATAAATTGTTCACTAACAGAt > 1:1972448/1‑76 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:1016583/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt > 2:1281194/1‑76 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:209639/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:1901768/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:609603/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑TAT‑TT‑CATCGCGAAATGGACACAGGATAAATTGTTCAATAACGGAtt < 1:26951/76‑1 (MQ=255)
aCCGCTTCATCGTGAAAAAACTACA‑T‑T‑T‑T‑T‑TTGCATCGTGAAATGGACAGAGGATAGCGTGTTCAATAACGGATTa < 1:23726/76‑1 (MQ=255)
aCCGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:920401/76‑1 (MQ=255)
ccGCTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCATGAAATGGTCAGAGGATAAATTGTTCAATAACGGATTAt > 1:38458/1‑76 (MQ=255)
cGCTTAACATTGAANAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 1:136276/1‑76 (MQ=255)
cTTCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc > 1:1384928/1‑76 (MQ=255)
ttCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc > 1:703512/1‑76 (MQ=255)
ttCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 2:511534/76‑1 (MQ=255)
gtCACCTTGAAAAAACTACA‑T‑T‑T‑T‑TCTT‑TATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 1:325553/75‑1 (MQ=255)
tCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 1:1206011/1‑76 (MQ=255)
tCACCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg > 2:1816671/1‑76 (MQ=255)
aCCTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGGTCAATAACGGATTATCCCCGCt < 2:802260/76‑1 (MQ=255)
ccTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 1:1429627/76‑1 (MQ=255)
ccTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg < 2:546952/76‑1 (MQ=255)
cTTGAAAAAACTACNTT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGAAAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:5954/1‑76 (MQ=255)
cTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:145421/1‑76 (MQ=255)
cTTGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 2:627906/1‑76 (MQ=255)
tGAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGCTTATCCCCGCTGACg > 1:1694618/1‑76 (MQ=255)
gAAAAAACTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa > 1:1046227/1‑76 (MQ=255)
aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCACACACGGATTATCCCCGCTGACGaa > 2:1421227/1‑76 (MQ=255)
aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 2:1956427/1‑76 (MQ=255)
aaaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa > 1:898438/1‑76 (MQ=255)
aaaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa > 2:689269/1‑76 (MQ=255)
aaaaCTACATT‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg > 2:1042375/1‑76 (MQ=255)
|
AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑T‑T‑T‑T‑T‑TT‑CATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAG > REL606/3875552‑3875691
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
