Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F1 I1 R1
|
15 |
26.4 |
1686296 |
95.9% |
1617157 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,875,632 |
(T)7→8 |
intergenic (‑66/+287) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,875,625 | 1 | . | T | 92.0%
| 81.8
/ 1.4
| 25 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (12/11); total (13/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCAT > REL606/3875552‑3875697
|
aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt > 1:549432/1‑76 (MQ=255)
attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑tt > 2:758166/1‑76 (MQ=255)
tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑tttt < 2:734900/76‑1 (MQ=255)
tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑tttt > 2:485022/1‑76 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑ttttt > 1:321825/1‑75 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑ttttt < 1:402955/76‑2 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑ttttt > 2:232841/1‑75 (MQ=255)
tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttt‑tttttca > 1:533403/1‑73 (MQ=255)
aCATACGCGTCCTGACTGTNANTAGAGAANGAATTTAAACCGCTTCACCTTNAANAAGCTACATTTT‑TTTTCATCg < 2:120535/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTg > 1:458389/1‑76 (MQ=255)
aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGaa < 1:445616/76‑1 (MQ=255)
gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAAt < 2:370436/76‑1 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGa > 2:365496/1‑76 (MQ=255)
tCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGa > 2:365490/1‑76 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCGCCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACa < 2:158024/76‑1 (MQ=255)
tGACTGTAATTTGAGAACGAATTTAAACCGCTGCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACag < 1:43557/76‑1 (MQ=255)
gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACaga > 2:416436/1‑76 (MQ=255)
cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAgg > 1:377320/1‑76 (MQ=255)
cTGTAATTTGAGAACGAATGTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAgg > 1:715252/1‑76 (MQ=255)
tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGa > 2:268978/1‑76 (MQ=255)
gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGt < 2:553574/76‑1 (MQ=255)
aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa < 1:630388/76‑1 (MQ=255)
aTTTAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc < 2:555616/76‑1 (MQ=255)
tttAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg > 1:670570/1‑76 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGGTCAATAACgg < 1:64395/76‑1 (MQ=255)
ttAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGACAAATTGTTCAATAACgg > 2:795701/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:40352/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACA‑TTTCTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:376346/1‑76 (MQ=255)
aaaCCGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt < 2:466257/76‑1 (MQ=255)
cGCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATc > 2:556089/1‑76 (MQ=255)
gCTTCACCTTGAAAAAACTACATTTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc > 1:20361/1‑76 (MQ=255)
aaaaCTACATTTT‑TTTTCATCGTGAAATGGACAGAGGANAATTTGTTCAATAACGGATTATCCCCGCTGACGAAAg < 1:823542/76‑1 (MQ=255)
tACA‑TTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCAt < 1:82995/76‑1 (MQ=255)
|
AGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTT‑TTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCCAT > REL606/3875552‑3875697
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
