Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
20 |
54.5 |
3547384 |
95.1% |
3373562 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,875,632 |
(T)7→8 |
intergenic (‑66/+287) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,875,625 | 1 | . | T | 96.4%
| 203.2
/ 0.7
| 57 | intergenic (‑59/+294) | glmU/atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (28/26); total (31/26) |
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 |
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGC > REL606/3875550‑3875694
|
aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAACACTACa > 2:573110/1‑76 (MQ=255)
tAGCATTATTCACCNGACGCGCCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t > 1:1712085/1‑76 (MQ=255)
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t < 2:1200123/76‑1 (MQ=255)
tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAAACGCTTCACCTTGAAAAAACTACA‑t < 1:1574798/76‑1 (MQ=255)
aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGGACGAATTTAAACTGCTTCACCTNGAAAAAACTACA‑tt < 1:4847/76‑1 (MQ=255)
attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt‑t > 1:1275160/1‑76 (MQ=255)
tattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt‑tt‑t < 2:126535/76‑1 (MQ=255)
attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt‑tt‑tt > 1:1680277/1‑75 (MQ=255)
tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt‑tt‑ttca < 1:611077/76‑4 (MQ=255)
cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGt > 1:978109/1‑76 (MQ=255)
cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGt > 1:774680/1‑76 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTg < 1:1381916/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTg < 1:1318840/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTg < 1:787253/76‑1 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTg > 1:537033/1‑76 (MQ=255)
aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTAACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTg > 2:619976/1‑76 (MQ=255)
aTACGCGTCCTGACTGAAACTTGANAACGAATTTAAACCGCTTCACATTGAAAAAACTACATTTTT‑TT‑TCATCGTg > 1:153990/1‑76 (MQ=255)
cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTGACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGaaa < 1:1495633/76‑1 (MQ=255)
cgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATg > 1:944017/1‑76 (MQ=255)
gTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATgg < 1:1176222/76‑1 (MQ=255)
ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGAc > 2:972981/1‑76 (MQ=255)
cTGACTGTAATTTGAGAATGANTTNAAACCGCTTCACCTTGAAAAANCTACACTNTN‑TT‑TCATCGTGAAATGGACa > 2:255285/1‑76 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACa < 1:521876/76‑1 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACa < 1:645392/76‑1 (MQ=255)
cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACa < 1:676616/76‑1 (MQ=255)
gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACaga > 2:1015191/1‑76 (MQ=255)
aCTGTAATTTGAGAACGAATTTAAACCGCTTGACNTTGAGAANACNACN‑TTTTGTT‑TGATCGTGAAATGGACagag > 1:1533910/1‑76 (MQ=255)
cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTTAACAAACTACANTTTT‑TT‑TCATCGTGAAATGGACAGAgg < 1:1553740/76‑1 (MQ=255)
cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTTCATTTTT‑TT‑TCATCGTGAAATGAACAGAgg > 1:26997/1‑76 (MQ=255)
cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAgg < 2:1273065/76‑1 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGANAGAGGAt < 2:1417916/76‑1 (MQ=255)
gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGAt < 1:1418829/76‑1 (MQ=255)
aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATaa > 1:819745/1‑76 (MQ=255)
aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATaa < 2:456995/76‑1 (MQ=255)
tttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAAt > 1:602791/1‑76 (MQ=255)
ttGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAAtt < 1:1642311/76‑1 (MQ=255)
agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGtt < 1:1640478/76‑1 (MQ=255)
aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCaa > 2:1357435/1‑76 (MQ=255)
cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAAt > 1:1362445/1‑76 (MQ=255)
aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 2:341393/1‑76 (MQ=255)
aaTTTAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATaa > 2:341378/1‑76 (MQ=255)
ntttAAACCGCTTCACCTTGAAAAAACTACA‑TTTT‑TTGTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc > 1:1479712/2‑76 (MQ=255)
tttAAACCGCTTCACCTNGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGAGAGAGGATAAACTGGTCAATAACg > 1:220763/1‑76 (MQ=255)
tAAACCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa > 2:189413/1‑76 (MQ=255)
aaaCCGCTACACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt > 1:1638013/1‑76 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:1163067/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 1:1236860/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt > 2:1290337/1‑76 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:516946/76‑1 (MQ=255)
aaCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt < 2:356570/76‑1 (MQ=255)
aCCGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa < 2:610036/76‑1 (MQ=255)
aCCGCTTCAACTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa > 2:694902/1‑76 (MQ=255)
ccGCTTCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt < 1:1611046/76‑1 (MQ=255)
ttCACCTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc < 2:127074/76‑1 (MQ=255)
ccTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg > 2:1396907/1‑76 (MQ=255)
cTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGANAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 1:1723510/1‑76 (MQ=255)
cTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 2:1384579/1‑76 (MQ=255)
cTTGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGa > 2:1367392/1‑76 (MQ=255)
ttGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 2:1671486/76‑1 (MQ=255)
ttGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 1:352184/76‑1 (MQ=255)
ttGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc < 1:384596/76‑1 (MQ=255)
tGAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg > 1:785655/1‑76 (MQ=255)
gAAAAAACTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa < 1:1410645/76‑1 (MQ=255)
aaaaaaCTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa < 2:1469752/76‑1 (MQ=255)
aaaCTACATTTTT‑TT‑TCATCGTGAAATNGACAGAGGTTATATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:1557244/1‑76 (MQ=255)
aaaCTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc > 1:1286014/1‑76 (MQ=255)
aCTACATTTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcgc > 1:1164302/1‑76 (MQ=255)
|
ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTT‑TT‑TCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGC > REL606/3875550‑3875694
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
