Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I1 R1 75 123.0 6181441 93.9% 5804373 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,494,883 (A)7→8 coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,494,8761.A98.2% 396.5 / ‑0.2 113coding (254/564 nt)idnKD‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (56/55);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 130 total aligned reads displayed.

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCA  >  NC_000913/4494777‑4494972
                                                                                                     |                                                                                                  
tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑Att                                                                                                     <  2:2464055/100‑1 (MQ=255)
 aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑a                                                                                                  <  1:2945205/100‑1 (MQ=255)
   tggatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaa                                                                                                <  2:480996/98‑1 (MQ=255)
    tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaa                                                                                                <  1:3086987/99‑1 (MQ=255)
     gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaa                                                                                              >  2:2319820/1‑100 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAGAAGAATGAAAAAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aac                                                                                         >  2:1501008/1‑97 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aaca                                                                                        >  2:59245/1‑97 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aaca                                                                                        <  1:1170329/100‑4 (MQ=255)
           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aacag                                                                                       <  2:893794/100‑5 (MQ=255)
            aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACCGTCTTTATAAAATGATTGGACTAGGATTTCTTGTCTGTTCATC‑ATT‑‑aaaaaccaccg                                                                                        >  1:1799959/1‑93 (MQ=255)
            aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aacagt                                                                                      >  2:3021458/1‑95 (MQ=255)
              cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTAt                                                                                     <  1:335758/100‑1 (MQ=255)
              cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTAt                                                                                     >  1:1616027/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATc                                                                                    >  2:2127933/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATc                                                                                    >  1:2008121/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATc                                                                                    >  1:442969/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑aaaaaa‑aaacagtat                                                                                   >  1:263667/1‑92 (MQ=255)
                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTAttg                                                                                   <  1:1341294/100‑3 (MQ=255)
                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCg                                                                                   <  2:18356/100‑1 (MQ=255)
                 cTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGt                                                                                  >  2:2794278/1‑100 (MQ=255)
                 cTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGt                                                                                  >  2:2092171/1‑100 (MQ=255)
                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGa                                                                                <  1:1888541/100‑1 (MQ=255)
                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGa                                                                                >  2:3022059/1‑100 (MQ=255)
                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGa                                                                                <  1:1084135/100‑1 (MQ=255)
                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGa                                                                                >  1:846295/1‑100 (MQ=255)
                     ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGata                                                                              >  1:2351567/1‑100 (MQ=255)
                     ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGata                                                                              >  2:1050181/1‑100 (MQ=255)
                       tggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGatat                                                                             <  2:442967/99‑1 (MQ=255)
                       tggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATAtt                                                                            >  2:2701430/1‑100 (MQ=255)
                       tggcaggAAGGATTAAAAGATGCTTCATACAGTCTTTATAAAAAAAATGAAACAGGATTTATTGTCTGAAAAAAAAAA‑‑AAAAAA‑ACAGTATCGTGat                                                                               <  2:2653970/97‑1 (MQ=255)
                         gctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATAttt                                                                           <  2:116192/99‑1 (MQ=255)
                          ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTa                                                                         <  2:846290/100‑1 (MQ=255)
                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAGAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTAc                                                                        >  2:1655030/1‑100 (MQ=255)
                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTAc                                                                        <  1:1135012/100‑1 (MQ=255)
                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTAc                                                                        <  1:1983397/100‑1 (MQ=255)
                               aCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCa                                                                     <  2:72256/99‑1 (MQ=255)
                               aCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCa                                                                     >  1:2482473/1‑99 (MQ=255)
                                cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAg                                                                   <  2:350676/100‑1 (MQ=255)
                                 gCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAgg                                                                  >  2:2477785/1‑100 (MQ=255)
                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑CTTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAggg                                                                 >  1:2162564/1‑100 (MQ=255)
                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAggg                                                                 <  1:2631191/100‑1 (MQ=255)
                                       atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCTTGATATTTTCCGCAGGGGTGGcc                                                            >  1:309791/1‑100 (MQ=255)
                                       atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                            >  1:309599/1‑100 (MQ=255)
                                         gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                          >  2:2228141/1‑100 (MQ=255)
                                          atgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGccccc                                                         <  2:1035167/100‑1 (MQ=255)
                                            gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTAAAAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                        <  2:263666/100‑1 (MQ=255)
                                            gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                       >  2:1325955/1‑100 (MQ=255)
                                            gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                       >  1:2388453/1‑100 (MQ=255)
                                             cTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                      <  1:3022091/100‑1 (MQ=255)
                                               tCATACAGTCTTTATAAAAAGAATTAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGCGTAGCCTCCATGtt                                                    <  2:648498/100‑1 (MQ=255)
                                               tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                    >  1:2191445/1‑100 (MQ=255)
                                                cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                   >  2:880491/1‑100 (MQ=255)
                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                                 >  1:2212042/1‑100 (MQ=255)
                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                                 >  2:1552222/1‑100 (MQ=255)
                                                   aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATA‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                                <  2:2460754/100‑1 (MQ=255)
                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                             <  1:557880/100‑1 (MQ=255)
                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                             <  1:1563375/100‑1 (MQ=255)
                                                          ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                         <  1:3012119/100‑1 (MQ=255)
                                                          ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                         >  1:593918/1‑100 (MQ=255)
                                                             taAAAAGAATTAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      <  2:2019686/100‑1 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      >  1:933863/1‑100 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      <  2:1598499/100‑1 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      <  1:433876/100‑1 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      <  1:1274001/100‑1 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      >  2:62474/1‑100 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                      >  2:693863/1‑100 (MQ=255)
                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                     <  2:183437/100‑1 (MQ=255)
                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                     >  2:1843058/1‑99 (MQ=255)
                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                    >  2:678248/1‑100 (MQ=255)
                                                                  aGAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                                 <  1:791801/100‑1 (MQ=255)
                                                                  aGAATGAAACAGGATTTATTGTCTGTTCATA‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                                 <  2:2296819/100‑1 (MQ=255)
                                                                   gAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg                                <  1:2701455/100‑1 (MQ=255)
                                                                   gAATGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg                                >  1:468627/1‑100 (MQ=255)
                                                                     aTGAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                              >  2:118470/1‑100 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                            <  1:1534568/100‑1 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                            <  1:1212407/100‑1 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                            <  1:1326111/100‑1 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                            >  1:1253608/1‑100 (MQ=255)
                                                                        aaaCAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                           <  1:943156/100‑1 (MQ=255)
                                                                         aaCAGTATTTATTGTCTGTACATC‑ATTA‑AAAAAA‑ACAGTATCGTGTTATTTTACGCAAGCGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                          <  2:293722/100‑1 (MQ=255)
                                                                         aaCAGGATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATGTTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                          >  2:706069/1‑100 (MQ=255)
                                                                           cAGGATTTATTGTCTTTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATg                        <  2:655467/100‑1 (MQ=255)
                                                                            aGGATTTATTGTCTGTTCATC‑AATA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCAACCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                       >  2:1792334/1‑100 (MQ=255)
                                                                             ggATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                      <  1:929415/100‑1 (MQ=255)
                                                                             ggATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                       >  2:440127/1‑99 (MQ=255)
                                                                              gATTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaaa                     <  1:2431338/100‑1 (MQ=255)
                                                                               aTTTATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                    >  2:545158/1‑100 (MQ=255)
                                                                                tttATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACt                   >  1:756803/1‑100 (MQ=255)
                                                                                  tATTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt                 <  2:2008099/100‑1 (MQ=255)
                                                                                   aTTGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAtt                <  1:1368766/100‑1 (MQ=255)
                                                                                     tGTCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct              >  1:425714/1‑100 (MQ=255)
                                                                                       tCTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg            <  2:1234249/100‑1 (MQ=255)
                                                                                        cTGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgc           >  1:671861/1‑100 (MQ=255)
                                                                                         tGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg          >  2:1138322/1‑100 (MQ=255)
                                                                                         tGTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg          >  2:1813783/1‑100 (MQ=255)
                                                                                          gTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc         >  2:2088007/1‑100 (MQ=255)
                                                                                          gTTCATC‑ATTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc         <  2:1616009/100‑1 (MQ=255)
                                                                                             catc‑atTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa      >  2:2331114/1‑100 (MQ=255)
                                                                                             catc‑atTA‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa      >  2:2042684/1‑100 (MQ=255)
                                                                                                  atT‑‑AAAAAACACAGTAGCGTGATATTTTACGCAAGGCTAGCCCCCATGGTCATTTCGACGGTTTAGATGGGGTATATGAAACTGTTTGCGCGTGAAGGCa  >  1:140864/1‑100 (MQ=255)
                                                                                                     |                                                                                                  
TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATC‑ATT‑‑AAAAAA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCA  >  NC_000913/4494777‑4494972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A