Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F59 I0 R1
|
37 |
24.7 |
1037378 |
89.7% |
930528 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 104.5
/ NA
| 34 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/18); total (16/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC > NC_000913/4184409‑4184676
|
gcttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGCCGTCACGGTAACAAGGGTGTAATTTCTAAGATCATCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg < 2:493141/141‑1 (MQ=255)
cggtggccAGACTGGTGCTGAGATGGGGGGTCGTAAGCGTAACAAGGGTGTAATGTCGAAAATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc < 2:204821/137‑1 (MQ=255)
tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCAGCTGGCCGTACCGTGTCGTATg > 1:150504/1‑143 (MQ=255)
cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa < 2:350167/143‑1 (MQ=255)
aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCAc > 2:317356/1‑143 (MQ=255)
ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg > 2:44059/1‑143 (MQ=255)
aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt < 1:219473/143‑1 (MQ=255)
aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGACGTACCGTCTCGTATGAACATCGCGCAGAt > 1:82709/1‑143 (MQ=255)
gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCCATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa < 1:500623/143‑1 (MQ=255)
cACGGTAACAAGGGTGTAATTGCTAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt < 2:48143/143‑1 (MQ=255)
cGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg > 1:402874/1‑143 (MQ=255)
cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg > 1:221242/1‑143 (MQ=255)
tggactttCTGAGATCGAGCCGATGGAAGATATGCCGTACGATTAAAACGGTACGCCGGTAGACATTGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGTGa < 2:495321/138‑1 (MQ=255)
tAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGTTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa < 2:214979/143‑1 (MQ=255)
tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATCAAAACGGTACGCCGGTAAACAACGTACGGAACCAGGTGGGCGTACCGTCTCGGATGCAGATCGGTCAGACCGTCCACACCCCCCTGGGTAGTGCTGCGAGAGGt > 2:190327/1‑143 (MQ=255)
tCAACCCGATCGAAGATATGCTTTACTATGAAAACGGTACGTTGGTATACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc < 1:419251/143‑1 (MQ=255)
cAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa < 1:44059/143‑1 (MQ=255)
ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTCTGAACATCGGTCAGATCCTCGAAACCCACCGGAGCATGGCTGCGAAGGGTCTCGGCGAccaaat > 2:416464/1‑138 (MQ=255)
cGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGCTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGGCAAGATc > 1:339737/1‑143 (MQ=255)
aTCGAAGATATGCCTTACGATGAGGACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa < 2:239935/143‑1 (MQ=255)
atcggCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg < 2:328219/139‑1 (MQ=255)
atGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAACAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGACTGGCCACGAAAGGTATCGACGCGCAGATACACGCCATgat > 1:37699/1‑141 (MQ=255)
tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCCCGCTg > 2:98881/1‑143 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa < 1:28822/143‑1 (MQ=255)
ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAGGATCAACGCCATGCTGGAAc > 1:316550/1‑143 (MQ=255)
gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca < 1:417351/143‑1 (MQ=255)
caaaCGGCAGCCCGGGAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAATCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa < 1:373176/142‑1 (MQ=255)
tgTAAGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt < 1:317228/142‑1 (MQ=255)
gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGCGAAAGGTATCGGCGGCAAGATCAACGCCATGCTGAAACAGCAGCACGAAGCcgggg > 1:514907/1‑140 (MQ=255)
gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt > 1:187101/1‑143 (MQ=255)
gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATAGAAACCCACCTGGGTATGGCTGCGAAAGGTAACGGCGAAAAGATCAACGCCCAGGGGAAACAGCAGAAAGAAGAcgacaacca > 2:143717/1‑136 (MQ=255)
aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAGCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAGGTCGCGAAACTGCGCGa > 1:400187/1‑143 (MQ=255)
tCGCACGGAACCAGCCGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa < 1:209091/143‑1 (MQ=255)
tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc < 1:455994/143‑1 (MQ=255)
|
GGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC > NC_000913/4184409‑4184676
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
