Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F21 I0 R1 92 47.7 1834152 89.2% 1636063 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA98.5% 171.1 / ‑5.7 63P1100Q (CCG→CAG) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (32/28);  minor base G (1/1);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184404‑4184677
                                                                                                                                           |                                                                                                                                      
cTGGCGCTTAAACGCATGATGCAGACTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                     <  2:499562/143‑1 (MQ=255)
    ttgTTAAACGCCGTATCCAGCCTGGTGATAAGATTGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAATACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                 <  2:868684/141‑1 (MQ=255)
    cGGTTAAACGCCGTATCGAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                 <  1:13442/143‑1 (MQ=255)
         aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGCACTGAACCAGCTGGGCGTAcc                                                                                                                            >  1:187333/1‑143 (MQ=255)
            cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCCTACGCTGAAAACGGCACGCCCGTAGACACCGTACTGAACCAGCCGAGCGTACCgcc                                                                                                                         >  2:400695/1‑141 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTACCAAGGGTGTAATTTCTTAGATTAACCCGATCGAAAATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                >  1:170437/1‑143 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATgca                                                                                                                >  2:522079/1‑141 (MQ=255)
                     cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                <  1:261981/143‑1 (MQ=255)
                     cAGCCTGGTGACAAGATGGCACGGCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                <  2:590793/143‑1 (MQ=255)
                      aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAACTTCTAAGATCAACCCGATCGCAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACCGAACCAGCTGGGCGGACCGTCTCGTCTGagc                                                                                                               >  2:161676/1‑141 (MQ=255)
                            gTGACAAGATGGCTGGTCCGCACGGTAACAAGGGTGTAATTTCTAAGATCAGCCCGATCGAAGATATGCCTTACGATGAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                         <  2:68423/143‑1 (MQ=255)
                             tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGGGTACCTGACGCCGGTAGACCTCGTACTGATCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                        >  1:336593/1‑143 (MQ=255)
                             tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                        >  1:282217/1‑143 (MQ=255)
                                 aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                    >  1:434802/1‑143 (MQ=255)
                                       gCAGGTCGTCCCGGTAATCAGGGTGTAATTTCTAAGATCGCCCCGATCGAAGATAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                              <  2:755632/143‑1 (MQ=255)
                                        cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                             <  1:192013/143‑1 (MQ=255)
                                         aGGTGGTCACGGGAGCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                            <  2:555654/143‑1 (MQ=255)
                                         aGGTCGTCACGGTAACAAGGGTGTAATTTCTACGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                            <  2:616668/143‑1 (MQ=255)
                                         aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAACTCGGTCACATCCTCgcca                                                                                            >  2:668429/1‑140 (MQ=255)
                                            tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAccc                                                                                         >  1:241023/1‑143 (MQ=255)
                                             cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAATCGGTAAGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                        <  1:465589/143‑1 (MQ=255)
                                              ggcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                       <  2:234607/141‑1 (MQ=255)
                                               tcACGGTAACAAGCGTGTAATTTCTAAGCTCAACCCGATCGAACATATGCCTTACGATGAAAACGCTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCCGACCCCCGAAACCCAcc                                                                                      >  2:319479/1‑143 (MQ=255)
                                                     tAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                >  1:613434/1‑143 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                            >  2:698713/1‑143 (MQ=255)
                                                         aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACCCACCCGCGTATGGc                                                                            >  2:30256/1‑143 (MQ=255)
                                                           gtgtgtAATTTCTAAGATTTATCCGATTTAATCTATGACTTACGATGAAAACGGTACGCCGGCAGACATCGTACGGAACCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTg                                                                          <  1:221913/141‑1 (MQ=255)
                                                            ggTGTAAATTCTGAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                         <  2:170437/143‑1 (MQ=255)
                                                            agtatAATTTCTAATATCAACCAGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                         <  1:115122/139‑1 (MQ=255)
                                                             gtgtAATTTCTAAGATCAACCCGATCGAAGATGTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTggg                                                                        >  1:188269/1‑141 (MQ=255)
                                                              tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTACGAACATCGGTCAGACCCCCGAAACCCACCCGCGTATGGCTGCGa                                                                       >  2:373990/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                     >  1:858971/1‑143 (MQ=255)
                                                                tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTACGGCTGCGaaa                                                                     >  2:710725/1‑143 (MQ=255)
                                                                  aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTCAGCGGGTTGTGGGTTTAATGGAGCGGCGAGGCGTACCGTCTCGTGTCAACATCGGTCACATCCTCGAAACCCACCCGGGTGTGGCTGCGgaatg                                                                   >  2:646976/1‑141 (MQ=255)
                                                                       tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGATCCTCGGAACCCACCGCGGTATGGCGGCGAAAGCGATCg                                                              >  2:109082/1‑143 (MQ=255)
                                                                         aGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                            <  2:55363/143‑1 (MQ=255)
                                                                           aTCACCCCGATCGAAGATATGCCTCACGATGAGATTGGTCCGCCCACAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                          <  2:399730/143‑1 (MQ=255)
                                                                           aTCAACCCGATCGAAGATATACCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGATGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCAACCCGGGTATGGCGTCGAAAGGTATcgacga                                                          >  2:23422/1‑143 (MQ=255)
                                                                            tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGATAGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAACCGAGCGGGGTATGGCTGCGAAAGGTGACCGCGAc                                                         >  2:456091/1‑143 (MQ=255)
                                                                              gaCCCGATCGAAGATATGCCCTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAGCATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                       <  2:618581/142‑1 (MQ=255)
                                                                              aaCCCCATCGAAGATATGCCTTACGATGAAAACGGTCCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                       <  1:213361/143‑1 (MQ=255)
                                                                                   gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATCGCTGCGAAAGGTATCGGCGACAAGATcc                                                  >  2:379600/1‑142 (MQ=255)
                                                                                     tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATCCCCGAAACCCACCCGGGCATGGCTGCGAAAGGTCTCGGGGCCAAGATCAAc                                                >  2:128179/1‑143 (MQ=255)
                                                                                       gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGc                                              >  1:448492/1‑143 (MQ=255)
                                                                                        aaGATATGCCTTACGATGAAAACGGTCCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATCAACATCGCTCAGATCCTCCAAACCCACCAGGATATGACTGCAACAGCTATCCGCGACAAGACCAACGcc                                             >  2:445042/1‑143 (MQ=255)
                                                                                         aGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTCTCGGCGACAAGATCAACGCCa                                            >  1:293150/1‑143 (MQ=255)
                                                                                              tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCGACGCCATGCTg                                       >  1:111886/1‑143 (MQ=255)
                                                                                               gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGCTCGCGGCCATGCGGa                                      >  2:295907/1‑143 (MQ=255)
                                                                                                    aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                 >  1:824956/1‑143 (MQ=255)
                                                                                                        gggaaaCGGTGCGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                             <  2:910098/140‑1 (MQ=255)
                                                                                                           aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATAAACGCCATGCTGAAACAGCAGCaag                          >  2:513914/1‑143 (MQ=255)
                                                                                                             ccGGTACCCCGGTATACTTCGTACTGAACCAGCTATGCGGACCGTCTCGTATGAACATCGGTCATATCCTCGAAACCCACCTGTGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                        <  2:735752/142‑1 (MQ=255)
                                                                                                              cGGTCCGCCGGTAGACATCGTACTGACCCCGCGGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                       <  2:571387/143‑1 (MQ=255)
                                                                                                                 tgcgcgGGTGGACAGCGTACTGAAGGAGCGGGGGGTACCGTCTCGTAGGAACAGCGGGCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                    <  1:30256/141‑1 (MQ=255)
                                                                                                                  ccGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                   <  1:801944/142‑1 (MQ=255)
                                                                                                                          aGACACCGTACTGAACCAGCTGGGCGTACCGACTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc           <  1:767104/143‑1 (MQ=255)
                                                                                                                              gtCGTACTGAACCGGCTAGGCGTACCGTCTCGTATAAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa       <  2:77073/142‑1 (MQ=255)
                                                                                                                               tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTGTGGCTGCGAAAGGTATCGGCGAGAAGATCAACGCCATGCTGAAACAGCCGCAAGACGTCGCGAAACTGCGCGaa      >  1:464820/1‑143 (MQ=255)
                                                                                                                               tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGCAACAGCAGCACGAAGCCGCGAAACTGCGCGaa      >  1:112714/1‑143 (MQ=255)
                                                                                                                                cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt     <  2:241023/143‑1 (MQ=255)
                                                                                                                                 gTACGGAACCAGCTGGGTGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTACGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt    <  1:522079/143‑1 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa  <  2:336593/143‑1 (MQ=255)
                                                                                                                                   aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa  <  2:282217/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA  >  NC_000913/4184404‑4184677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A