Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F52 I0 R1
|
35 |
30.0 |
1250572 |
90.4% |
1130517 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
4,184,543 |
C→A |
100% |
P1100Q (CCG→CAG) |
rpoB → |
RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0%
| 90.8
/ NA
| 32 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/23); total (9/23) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC > NC_000913/4184405‑4184676
|
tggctagtAAACGCCGCAAACAGCCTCGTGACTAGCTGGCACGTATTCACGTGAACAACGGTGTAATTTCTCAGTTCATCCCTATCGAAGCTACGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTg < 2:160511/136‑1 (MQ=255)
ggCGGTTAAACGCCGTACCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg < 1:569879/143‑1 (MQ=255)
cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGTTCATCTCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc < 1:161805/143‑1 (MQ=255)
tgcgcGCCGGATCCAGCCAGGTGACAAGAAGGCAGGGCGTCACGGTAACAAGGGTGTAATTTCTAAGCACAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc < 1:94952/139‑1 (MQ=255)
aaGATGGCAGGTCGCCACGGTAACAAGGTTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATTGGTCAGAt < 1:367402/143‑1 (MQ=255)
agagGGCAGGCCGTCACGGTAACAAGTGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTACGGGTGACATTGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc < 2:376281/143‑1 (MQ=255)
gTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGACCATCGGTCAGATCCTCGAACCCCACCTggct > 2:21034/1‑141 (MQ=255)
aagacggggGTAATTTCCAAGATCAACCCGATCGAAGATATGCCTTACGATGACACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt < 2:376923/138‑1 (MQ=255)
aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATACCTTACGATGAAAACGGGACGCCGGTAGACATCGTACAGAACCAGCTGGACGTACCGTCTCATATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt < 1:11871/143‑1 (MQ=255)
aaGGGGGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc < 2:423146/143‑1 (MQ=255)
aaGGCTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc < 2:427578/143‑1 (MQ=255)
tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAAAAGCTGGGCGTAACGTCACGTACGAAAATCGATCACAACATGGGATCCCACCTGGGGATGGCTGCGa > 1:257762/1‑143 (MQ=255)
aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg < 1:133571/143‑1 (MQ=255)
cTAAGGTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc < 2:164116/143‑1 (MQ=255)
cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc < 1:19001/143‑1 (MQ=255)
tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGACGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc > 1:351925/1‑143 (MQ=255)
aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGATGGGTAAGGTATCGGCGACAAg < 2:546596/143‑1 (MQ=255)
ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt < 1:527445/143‑1 (MQ=255)
tCGAAGATGTGCCTTATGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCCGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc < 1:200297/143‑1 (MQ=255)
aGATATGCCCTACGCTGAACACGGTCCGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTTCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTCGCTCTTCCATCGAAAGGTATCGGCGACAAGATCAACGCCa < 2:74521/143‑1 (MQ=255)
tatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc < 1:362138/143‑1 (MQ=255)
gCCTTACGACGAAAACGGCACGCCCGTAGCCATCGTACTGACCCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa < 1:13943/143‑1 (MQ=255)
ccTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGaa > 1:117529/1‑143 (MQ=255)
cTTACGATGAAAGCGGCACGCCGGTAGACACCGTACCGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa < 2:146353/143‑1 (MQ=255)
gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGTATGTCTGCGAAAGGTATCGCCTCCAAGGTCATCGCCATTGCGAAAcagca > 1:470210/1‑143 (MQ=255)
gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca < 1:135596/143‑1 (MQ=255)
aaaaCGGTACGCCGGTAGACCGCGTACGGAGCCAGAACCAACTTTCGTGTCGTATGAACATCGGTCAGATCTTCGAAACCTTCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa < 1:616258/143‑1 (MQ=255)
gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGAAAGGTATCGGCGACAACATCAACCCCATGCTGAAACAGCAGCAAGAAGTc > 2:135609/1‑143 (MQ=255)
cGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAACGTAAAAAGAACAGAAATTACGCCATGCTGAAACAGCAGCAAGAAGAcgcg > 2:439314/1‑143 (MQ=255)
gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcca > 1:3404/1‑141 (MQ=255)
aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc < 1:469683/143‑1 (MQ=255)
tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGGGCGAATTc > 1:112212/1‑143 (MQ=255)
|
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC > NC_000913/4184405‑4184676
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
