Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F83 I1 R1	24	40.7	1567444	90.4%	1416969	141.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	177.9 / NA	56	P1100Q (CCG→CAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (36/20); total (36/20)

TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184405‑4184678

tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                     <  1:580580/143‑1 (MQ=255)

   cGGTTAAGCGCCGTAGCGCGTCTGGTGACAAGATGGCATGTCGTCACGGTAACAAGGGTGTAATTTCTAACATCATCCCTTTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGc                                                                                                                                  <  2:722943/143‑1 (MQ=255)

      ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                               <  1:690443/143‑1 (MQ=255)

           cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGAAGACATCGTACTGAACCAGCCGGGCGACCCGtc                                                                                                                          >  2:396152/1‑143 (MQ=255)

           cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGCATGTAATTTCTAAGATCAACCCGATAGAAGATATGCCATACGATGAAAACGGAACGGCGCTAAAAATCGGACTAAAACAGCTAGGCgcaacgac                                                                                                                          >  2:127400/1‑136 (MQ=255)

            gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtcg                                                                                                                         >  1:307197/1‑142 (MQ=255)

            gCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGCTAGACATCGTACTGAACCAGCTGGGCTTATCgcaa                                                                                                                         >  2:454895/1‑140 (MQ=255)

             cggTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                        <  2:443105/141‑1 (MQ=255)

                tcgtcAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                     <  2:666735/139‑1 (MQ=255)

                   ccAGCCTGGTCAACGGATGGCAGGGCGTCAGGGTAACAAGGGTCTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGa                                                                                                                  <  2:415486/143‑1 (MQ=255)

                    cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                 >  1:419320/1‑143 (MQ=255)

                       ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt                                                                                                              >  1:277347/1‑143 (MQ=255)

                         cggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                            <  2:769577/142‑1 (MQ=255)

                                aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGCCCGGCTCGTCTGAACGTCGGTCAGAt                                                                                                     >  2:716464/1‑143 (MQ=255)

                                  gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                            >  2:494489/1‑134 (MQ=255)

                                  gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                            <  1:494489/134‑1 (MQ=255)

                                    tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                 <  1:316611/143‑1 (MQ=255)

                                     ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                                >  1:538490/1‑143 (MQ=255)

                                      gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGCAGATATAACTTCAGATGAAGATGGTACCCCGGTAGACATCGTACTGAACCAGCTCGGCGTACAGTCTCGCATGCACGTCGGTCCGGTCCTCGa                                                                                               >  1:700632/1‑143 (MQ=255)

                                      gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACACGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGAATAAAAAGCGGTCAGAACCTCGa                                                                                               >  2:187000/1‑143 (MQ=255)

                                              tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGACCCTCGCAAcccccc                                                                                       >  2:137891/1‑143 (MQ=255)

                                              tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGAATCGTAAGAACAACGGCCAGATCGACGGAAccgcca                                                                                       >  2:176118/1‑139 (MQ=255)

                                               cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGACGTACCGTCTCGTATGCAAATCGGGCAGATCCTCGAGACCCAcca                                                                                      >  2:746888/1‑142 (MQ=255)

                                               cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                      <  1:478928/143‑1 (MQ=255)

                                                 cGGTAACAAGGGTGTAATTCCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGTACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTgg                                                                                    <  2:275689/143‑1 (MQ=255)

                                                       cAAGGGTGTAATTTCTAAGCTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                              <  1:693359/143‑1 (MQ=255)

                                                       cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                              <  2:220519/143‑1 (MQ=255)

                                                         aGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                            <  1:509899/143‑1 (MQ=255)

                                                            gtgtAATTTCTAAGTTGAACCGGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCg                                                                         <  2:59904/143‑1 (MQ=255)

                                                              gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATGGGTCAGATGCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                       >  2:200449/1‑143 (MQ=255)

                                                                 aTTTCTAAGATCAACCCGATCGAAGATATGCCTTNCGATGANAACGGTNCGCCGGTAGACATCGTACNGAACCAGCTGGGCGTACCGTCCCGTCTGANCATCGCTCAGATCCTCGACACCCACCTGGCTATGGCCGCCACAgg                                                                    >  2:405408/1‑143 (MQ=255)

                                                                    tCTAAGATCGACCCGATCGAAGATATGCCTTACGATGAGAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                                                 <  2:695417/143‑1 (MQ=255)

                                                                         gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCg                                                            >  2:64657/1‑143 (MQ=255)

                                                                             cgcccGTTCGAAGACATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                        <  1:205353/141‑1 (MQ=255)

                                                                                ccGATTGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGAt                                                     >  1:698756/1‑143 (MQ=255)

                                                                                ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaggga                                                     >  2:158695/1‑139 (MQ=255)

                                                                                  gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTGTGGCTGCGAGAGGTATCGGGGACAAGAGCa                                                   >  2:611613/1‑143 (MQ=255)

                                                                                      gAAGATATGCGTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                               <  1:646745/143‑1 (MQ=255)

                                                                                      gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                               >  1:644103/1‑143 (MQ=255)

                                                                                       aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                              >  1:258764/1‑143 (MQ=255)

                                                                                                 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                    >  1:474957/1‑143 (MQ=255)

                                                                                                 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                    >  1:377000/1‑143 (MQ=255)

                                                                                                     gATGAAAACGGTACGCCAGTAGACATCGTACAGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                <  1:120678/143‑1 (MQ=255)

                                                                                                       tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTTCGAAAGGTATCGGCGACAAGATCAACCCCCTGCTGAAACagcagc                              >  2:211379/1‑143 (MQ=255)

                                                                                                       tGAAAACGGTACGCCGGTAGACATCGTACTGAAAAAGCTGGGCGTACCGTGTCGTATGAACATAGGTCAGATCCTCGAAAGCAAACTGGCTATGGCTGCGAAAGGTAACGGAGAGAAGATCAACGAAATGCTGAAAcagcaga                              >  2:218409/1‑142 (MQ=255)

                                                                                                        gAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                             >  2:269692/1‑143 (MQ=255)

                                                                                                             cGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCacgaag                        >  1:130601/1‑143 (MQ=255)

                                                                                                               gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCACGAAACCGACCTGGGTATGGCTGCGAAAGGTATCGGGGACCAGATCAACGCCGTGCTGAAGCAGCAGCaagcacac                      >  2:512295/1‑138 (MQ=255)

                                                                                                                   gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGCTCACCGCCACGCTGCACCAGCCGCAAGCCGTcgcgc                  >  1:252502/1‑142 (MQ=255)

                                                                                                                     cGGTAGACATCGTACTGAACTAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                >  2:623338/1‑143 (MQ=255)

                                                                                                                     cGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCACGAGGTCGCGaaa                >  1:40363/1‑143 (MQ=255)

                                                                                                                       gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCGAGAAGTCGCGAAAcc              >  2:675541/1‑142 (MQ=255)

                                                                                                                           aCAGCGTACTGGACCAGGTGGGCGTACCGTCTCGTATGAACATCGGTCAGATGCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc          <  2:252502/143‑1 (MQ=255)

                                                                                                                              tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa       >  1:552249/1‑143 (MQ=255)

                                                                                                                                  aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCCAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAACCTGCGCGGATTcc   >  1:295192/1‑142 (MQ=255)

                                                                                                                                   cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGACGTCGCGCAACTGCGCGACTTCAt  >  1:38882/1‑143 (MQ=255)

TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT  >  NC_000913/4184405‑4184678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A